LOCUS       4_pBac_6xP3_TcHspE        6455 bp ds-DNA   circular     28-OCT-2016
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(1081..2133)
                     /vntifkey="21"
                     /label=pBacR
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
     misc_feature    3375..4097
                     /vntifkey="21"
                     /label=pBacL
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
     misc_feature    2111..2130
                     /vntifkey="21"
                     /label=p3E1.2Fwd
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
     misc_feature    3398..3418
                     /vntifkey="21"
                     /label=p3E1.2\Rv
                     /ApEinfo_fwdcolor=#ff80ff
                     /ApEinfo_revcolor=#ff80ff
     misc_signal     2412..3131
                     /vntifkey="87"
                     /label=ECFP
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     promoter        2245..2395
                     /vntifkey="29"
                     /label=TcHsp
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     polyA_signal    3142..3364
                     /vntifkey="25"
                     /label=SV40
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    2196..2242
                     /label=3xP3
                     /ApEinfo_fwdcolor=#ffc0cb
                     /ApEinfo_revcolor=#ffc0cb
     misc_feature    2144..2195
                     /label=3xP3(1)
                     /ApEinfo_label=3xP3
                     /ApEinfo_fwdcolor=#ffc0cb
                     /ApEinfo_revcolor=#ffc0cb
     misc_feature    5395..6255
                     /label=Amp(R)
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttgttta aaatataaca
      421 aaattgtgat cccacaaaat gaagtggggc aaaatcaaat aattaactag tgtccgtaaa
      481 cttgttggtc ttcaactttt tgaggaacac gttggacggc aaatcgtgac tataacacaa
      541 gttgatttaa taattttagc caacacgtcg ggctgcgtgt tttttgcgct ctgtgtacac
      601 gttgattaac tggtcgatta aataatttaa tttttggttc ttctttaaat ctgtgatgaa
      661 attttttaaa ataactttaa attcttcatt ggtaaaaaat gccacgtttt gcaacttgtg
      721 agggtctaat atgaggtcaa actcagtagg agttttatcc aaaaaagaaa acatgattac
      781 gtctgtacac gaacgcgtat taacgcagag tgcaaagtat aagagggtta aaaaatatat
      841 tttacgcacc atatacgcat cgggttgata tcgttaatat ggatcaattt gaacagttga
      901 ttaacgtgtc tctgctcaag tctttgatca aaacgcaaat cgacgaaaat gtgtcggaca
      961 atatcaagtc gatgagcgaa aaactaaaaa ggctagaata cgacaatctc acagacagcg
     1021 ttgagatata cggtattcac gacagcaggc tgaataataa aaaaattaga aactattatt
     1081 taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgc gtaaaattga
     1141 cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat agatattaag
     1201 ttttattata tttacactta catactaata ataaattcaa caaacaattt atttatgttt
     1261 atttatttat taaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt
     1321 aaacattctc tcttttacaa aaataaactt attttgtact ttaaaaacag tcatgttgta
     1381 ttataaaata agtaattagc ttaacttata cataatagaa acaaattata cttattagtc
     1441 agtcagaaac aactttggca catatcaata ttatgctctc gacaaataac ttttttgcat
     1501 tttttgcacg atgcatttgc ctttcgcctt attttagagg ggcagtaagt acagtaagta
     1561 cgttttttca ttactggctc ttcagtactg tcatctgatg taccaggcac ttcatttggc
     1621 aaaatattag agatattatc gcgcaaatat ctcttcaaag taggagcttc taaacgctta
     1681 cgcataaacg atgacgtcag gctcatgtaa aggtttctca taaatttttt gcgactttga
     1741 accttttctc ccttgctact gacattatgg ctgtatataa taaaagaatt tatgcaggca
     1801 atgtttatca ttccgtacaa taatgccata ggccacctat tcgtcttcct actgcaggtc
     1861 atcacagaac acatttggtc tagcgtgtcc actccgcctt tagtttgatt ataatacata
     1921 accatttgcg gtttaccggt actttcgttg atagaagcat cctcatcaca agatgataat
     1981 aagtatacca tcttagctgg cttcggttta tatgagacga gagtaagggg tccgtcaaaa
     2041 caaaacatcg atgttcccac tggcctggag cgactgtttt tcagtacttc cggtatctcg
     2101 cgtttgtttg atcgcacggt tcccacaatg gttccatggg acgtcgacga tctaattcaa
     2161 ttagagacta attcaattag agctaattca attaagatct aattcaatta gagactaatt
     2221 caattagagc taattcaatt aatccgtttc atatataagc gcggtctcgc ggcgcgttgt
     2281 cagtgaattt gaatcaagcg aagcgaaagc aacaaagcta agcaagcgac tcaaagcaaa
     2341 ttttcaaagc gactttaaca aagttgagtg aattatttac tagtgaattc aaagtggtac
     2401 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2461 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2521 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2581 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg
     2641 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     2701 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     2761 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     2821 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca
     2881 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg
     2941 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3001 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3061 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3121 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     3181 tttacttgct ttaaaaaacc tcccacactt ccccctgaac ctgaaacata aaatgaatgc
     3241 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3301 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3361 catcatgtat cttagatctg acaatgttca gtgcagagac tcggctacgc ctcgtggact
     3421 ttgaagttga ccaacaatgt ttattcttac ctctaatagt cctctgtggc aaggtcaaga
     3481 ttctgttaga agccaatgaa gaacctggtt gttcaataac attttgttcg tctaatattt
     3541 cactaccgct tgacgttggc tgcacttcat gtacctcatc tataaacgct tcttctgtat
     3601 cgctctggac gtcatcttca cttacgtgat ctgatatttc actgtcagaa tcctcaccaa
     3661 caagctcgtc atcgctttgc agaagagcag agaggatatg ctcatcgtct aaagaactac
     3721 ccattttatt atatattagt cacgatatct ataacaagaa aatatatata taataagtta
     3781 tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc
     3841 acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg
     3901 acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct
     3961 aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat
     4021 gcgtcaattt tacgcagact atctttctag ggttaaaaaa gatttgcgct ttactcgacc
     4081 taaactttaa acacgtcata gaatcttcgt ttgacaaaaa ccacattgtg gccaagctgt
     4141 gtgacgcgac gcgcgctaaa gaatggcaaa ccaagtcgcg cgagcgtcga ctctagagga
     4201 tccccgggta ccgagctcga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4261 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4321 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4381 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4441 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4501 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4561 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4621 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4681 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4741 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4801 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     4861 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4921 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4981 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     5041 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     5101 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     5161 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     5221 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5281 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5341 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5401 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5461 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5521 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5581 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5641 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5701 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5761 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5821 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5881 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5941 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     6001 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     6061 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     6121 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     6181 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6241 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6301 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6361 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6421 ctataaaaat aggcgtatca cgaggccctt tcgtc  
//
