LOCUS Exported 10885 bp ds-DNA circular SYN 13-DEC-2016 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS GFP_P2A_S.17.3_pLenti_CMV_Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10885) AUTHORS Jack Rose TITLE Direct Submission JOURNAL Exported Dec 13, 2016 from SnapGene Viewer 3.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source join(1..5471,7752..10885) /organism="synthetic DNA construct" /mol_type="other DNA" /label=source:synthetic DNA construct /note="source:synthetic DNA construct" promoter 27..131 /gene="bla" /label=bla /note="bla AmpR promoter" /note="AmpR promoter" CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=beta-lactamase /note="bla" /note="AmpR;confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="ori" /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="E. coli catabolite activator protein CAP bindin" /note="CAP binding site;CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="lac promoter" /note="lac promoter;promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="lac repressor encoded by lacI lac operator" /note="lac operator;The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2137..2153 /label=M13 rev /note="M13 rev" /note="M13 rev;common sequencing primer, one of multiple similar variants" promoter 2174..2192 /label=T3 promoter /note="T3 promoter" /note="T3 promoter;promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="5' LTR (truncated)" /note="5' LTR (truncated);truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="HIV-1 Psi" /note="HIV-1 Psi;packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="RRE" /note="RRE;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 4012..4129 /label=cPPT/CTS /note="cPPT/CTS" /note="cPPT/CTS;central polypurine tract and central termination sequence of HIV-1" enhancer 4152..4455 /label=CMV enhancer /note="CMV enhancer" /note="CMV enhancer;human cytomegalovirus immediate early enhancer" promoter 4456..4659 /label=CMV promoter /note="CMV promoter" /note="CMV promoter;human cytomegalovirus (CMV) immediate early promoter" CDS 4755..5471 /codon_start=1 /product="enhanced GFP" /label=enhanced GFP /note="enhanced GFP" /note="EGFP;mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" misc_feature 5472..5480 /label=P2A flanking sequence (probably not needed...)(1) /note="P2A flanking sequence (probably not needed...)(1)" misc_feature 5481..5537 /label=P2A /note="P2A" misc_feature 5538..5549 /label=P2A flanking sequence (probably not needed...) /note="P2A flanking sequence (probably not needed...)" misc_feature 5550..5552 /label=Start codon /note="Start codon" misc_feature join(5553..5606,5610..5633,5685..7673) /label=CIAR (aka S.17.3) /note="CIAR (aka S.17.3)" misc_feature 5553..5633 /label=BAD /note="BAD" misc_feature 5595..5597 /label=L151 /note="L151" misc_feature 5607..5609 /label=S155A /note="S155A" misc_feature 5609 /label=SOS_17-6_T968L_F158G /note="SOS_17-6_T968L_F158G" misc_feature 5616..5618 /label=F158G /note="F158G" misc_feature 5634..5684 /label=N-term linker /note="N-term linker" misc_feature 6753..6755 /label=F929 /note="F929" misc_feature 6870..6872 /label=T968L /note="T968L" misc_feature 7023..7031 /label=C-term linker /note="C-term linker" misc_feature 7032..7673 /label=BCL-XL_res_2-215 /note="BCL-XL_res_2-215" misc_feature 7674..7751 /label=HRAS_CaaX(c-term-25-residues) /note="HRAS_CaaX(c-term-25-residues)" misc_feature 7749..7751 /label=Stop /note="Stop" misc_feature 7767..8355 /label=WPRE /note="WPRE" /note="WPRE;woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(8238..8249) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa recognition and cleavage site /note="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" promoter 8369..8868 /label=PGK promoter /note="PGK promoter" /note="PGK promoter;mouse phosphoglycerate kinase 1 promoter" CDS 8889..9488 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=puromycin N-acetyltransferase /note="pac from Streptomyces alboniger" /note="PuroR;confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" LTR 9626..9859 /label=3' LTR (Delta-U3) /note="3' LTR (Delta-U3)" /note="3' LTR (Delta-U3);self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9931..10052 /label=SV40 poly(A) signal /note="SV40 poly(A) signal" /note="SV40 poly(A) signal;SV40 polyadenylation signal" rep_origin 10071..10206 /label=SV40 ori /note="SV40 ori" /note="SV40 ori;SV40 origin of replication" promoter complement(10239..10257) /label=T7 promoter /note="T7 promoter" /note="T7 promoter;promoter for bacteriophage T7 RNA polymerase" primer_bind complement(10267..10283) /label=M13 fwd /note="M13 fwd" /note="M13 fwd;common sequencing primer, one of multiple similar variants" rep_origin 10428..10883 /direction=RIGHT /label=f1 ori /note="f1 ori" /note="f1 ori;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc 4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaggatc 4741 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 4801 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 4861 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 4921 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 4981 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 5041 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 5101 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 5161 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 5221 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 5281 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 5341 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 5401 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 5461 agctgtacaa gggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg 5521 aggagaaccc tggacctgca ccaggaagta tggctcctcc taatctgtgg gcggcacagc 5581 gttatgggcg tgaactgcgg cgtatggccg atgagggtga gggttccttc aagggtagcg 5641 gaactgggtc tgggactggg agtggcaccg gttcagggac aggagatgtg tatcgctttg 5701 ctgagcctga ctccgaagag aacattatct tcgaagagaa tatgcagcct aaagctggta 5761 ttccgattat caaagccggg accgtgatta agctgatcga gcgtctgaca tatcacatgt 5821 acgccgaccc caactttgtt cgcaccttcc tgaccacata tcgttccttt tgcaagccac 5881 aggaactgct gtctctgatt atcgagcggt tcgaaattcc agaaccagag ccaaccgagg 5941 cagatcgtat tgcgatcgaa aacggcgacc agcctctgag cgcggagctg aaacgttttc 6001 ggaaggaata catccagccg gtccagctgc gggttctgaa tgtctgtcgc cattgggtgg 6061 agcaccattt ttatgatttc gaacgggacg cttacctgct gcagcgcatg gaagagttca 6121 ttggtacagt ccgcgggaaa gccatgaaaa agtgggtgga atccattact aagattatcc 6181 agcgcaaaaa gatcgcccgt gataacgggc caggacacaa tatcacattt cagagctccc 6241 cgcccactgt ggagtggcac atttctcgtc ccggtcatat cgaaaccttc gatctgctga 6301 cactgcatcc aattgagatc gctcgtcagc tgacactgct ggaatctgac ctgtatcgtg 6361 cagtgcagcc aagcgagctg gtgggctccg tttggaccaa agaggataag gaaattaaca 6421 gtccaaatct gctgaaaatg atccgccaca ctaccaacct gactctgtgg tttgaaaagt 6481 gcattgttga aaccgagaat ctggaagagc gtgtcgcggt ggttagccgg attatcgaga 6541 tcctgcaggt gtttcaggaa ctgaacaatt tcaacggcgt tctggaggtc gtgtccgcaa 6601 tgaacagcag ccctgtgtat cgcctggacc ataccttcga acagattccg agccgtcaga 6661 aaaagatcct ggaagaggcg cacgagctgt ccgaagatca ttataaaaag tacctggcaa 6721 aactgcgctc tattaaccca ccttgcgtgc cgtttttcgg tatttacctg actaacatcc 6781 tgaagaccga agagggaaat ccggaggttc tgaaacgtca cggcaaggaa ctgatcaact 6841 tttcaaaacg ccgtaaggtc gccgagattc tcggtgaaat ccagcagtat cagaatcagc 6901 cttactgtct gcgtgtggag agcgacatta aacggttttt cgaaaacctg aatccgatgg 6961 ggaacagtat ggaaaaagag tttaccgatt acctgttcaa taagtcactg gaaatcgagc 7021 ctggctctgg ttcacagagc aaccgtgaac tggttgtcga ttttctgtcc tataaactgt 7081 ctcagaaggg ctactcctgg tctcagttca gcgacgtcga agagaatcgg acagaggctc 7141 cggaaggtac tgaatctgag atggaaaccc ccagtgccat taacgggaat ccatcatggc 7201 acctggcaga tagccccgct gttaatggag ccaccggaca ttcgagctcc ctggacgccc 7261 gcgaggttat cccaatggcc gcagtcaaac aggcgctgcg tgaagcagga gatgagtttg 7321 aactgcgcta tcggcgcgcg ttcagtgacc tgacctcaca gctgcacatt acacccggca 7381 ctgcatacca gagttttgag caggtggtta acgaactgtt ccgcgacgga gtgaattggg 7441 gccgtatcgt tgcgtttttc agttttggcg gtgcactgtg tgtcgagtca gtggataaag 7501 aaatgcaggt cctggtgtct cgtattgcag cttggatggc aacttatctg aacgatcatc 7561 tggagccgtg gattcaggaa aatgggggat gggacacctt cgtggaactg tacggcaaca 7621 atgcggcggc tgagagtcgt aaggggcagg agcggttcaa tcgttggttt ctgcagcaca 7681 agctgcggaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg 7741 tgctctcctg aagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat 7801 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 7861 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 7921 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 7981 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 8041 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 8101 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 8161 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 8221 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 8281 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 8341 gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct 8401 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct 8461 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc 8521 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg 8581 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc 8641 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc 8701 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc 8761 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag 8821 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca 8881 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 8941 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 9001 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 9061 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 9121 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 9181 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 9241 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 9301 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 9361 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 9421 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 9481 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg 9541 catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 9601 cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat 9661 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 9721 ggctgcctag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 9781 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 9841 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 9901 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 9961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 10021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 10081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 10141 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 10201 gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc 10261 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 10321 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 10381 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag 10441 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 10501 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 10561 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 10621 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 10681 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 10741 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 10801 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 10861 caaaatatta acgtttacaa tttcc //