LOCUS       Exported               10885 bp ds-DNA     circular SYN 13-DEC-2016
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    GFP_P2A_S.17.3_pLenti_CMV_Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Jack Rose
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2016 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..5471,7752..10885)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /note="source:synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=bla
                     /note="bla AmpR promoter"
                     /note="AmpR promoter"
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="bla"
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="E. coli catabolite activator protein CAP bindin"
                     /note="CAP binding site;CAP binding activates transcription
                     in the presence of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="lac promoter"
                     /note="lac promoter;promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac repressor encoded by lacI lac operator"
                     /note="lac operator;The lac repressor binds to the lac 
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="M13 rev"
                     /note="M13 rev;common sequencing primer, one of multiple 
                     similar variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="T3 promoter"
                     /note="T3 promoter;promoter for bacteriophage T3 RNA 
                     polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="5' LTR (truncated)"
                     /note="5' LTR (truncated);truncated 5' long terminal repeat
                     (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="HIV-1 Psi"
                     /note="HIV-1 Psi;packaging signal of human immunodeficiency
                     virus type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="RRE"
                     /note="RRE;The Rev response element (RRE) of HIV-1 allows 
                     for Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4012..4129
                     /label=cPPT/CTS
                     /note="cPPT/CTS"
                     /note="cPPT/CTS;central polypurine tract and central 
                     termination sequence of HIV-1"
     enhancer        4152..4455
                     /label=CMV enhancer
                     /note="CMV enhancer"
                     /note="CMV enhancer;human cytomegalovirus immediate early 
                     enhancer"
     promoter        4456..4659
                     /label=CMV promoter
                     /note="CMV promoter"
                     /note="CMV promoter;human cytomegalovirus (CMV) immediate 
                     early promoter"
     CDS             4755..5471
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=enhanced GFP
                     /note="enhanced GFP"
                     /note="EGFP;mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5472..5480
                     /label=P2A flanking sequence (probably not needed...)(1)
                     /note="P2A flanking sequence (probably not needed...)(1)"
     misc_feature    5481..5537
                     /label=P2A
                     /note="P2A"
     misc_feature    5538..5549
                     /label=P2A flanking sequence (probably not needed...)
                     /note="P2A flanking sequence (probably not needed...)"
     misc_feature    5550..5552
                     /label=Start codon
                     /note="Start codon"
     misc_feature    join(5553..5606,5610..5633,5685..7673)
                     /label=CIAR (aka S.17.3)
                     /note="CIAR (aka S.17.3)"
     misc_feature    5553..5633
                     /label=BAD
                     /note="BAD"
     misc_feature    5595..5597
                     /label=L151
                     /note="L151"
     misc_feature    5607..5609
                     /label=S155A
                     /note="S155A"
     misc_feature    5609
                     /label=SOS_17-6_T968L_F158G
                     /note="SOS_17-6_T968L_F158G"
     misc_feature    5616..5618
                     /label=F158G
                     /note="F158G"
     misc_feature    5634..5684
                     /label=N-term linker
                     /note="N-term linker"
     misc_feature    6753..6755
                     /label=F929
                     /note="F929"
     misc_feature    6870..6872
                     /label=T968L
                     /note="T968L"
     misc_feature    7023..7031
                     /label=C-term linker
                     /note="C-term linker"
     misc_feature    7032..7673
                     /label=BCL-XL_res_2-215
                     /note="BCL-XL_res_2-215"
     misc_feature    7674..7751
                     /label=HRAS_CaaX(c-term-25-residues)
                     /note="HRAS_CaaX(c-term-25-residues)"
     misc_feature    7749..7751
                     /label=Stop
                     /note="Stop"
     misc_feature    7767..8355
                     /label=WPRE
                     /note="WPRE"
                     /note="WPRE;woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8238..8249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa recognition and cleavage site
                     /note="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     promoter        8369..8868
                     /label=PGK promoter
                     /note="PGK promoter"
                     /note="PGK promoter;mouse phosphoglycerate kinase 1 
                     promoter"
     CDS             8889..9488
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=puromycin N-acetyltransferase
                     /note="pac from Streptomyces alboniger"
                     /note="PuroR;confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             9626..9859
                     /label=3' LTR (Delta-U3)
                     /note="3' LTR (Delta-U3)"
                     /note="3' LTR (Delta-U3);self-inactivating 3' long terminal
                     repeat (LTR) from HIV-1"
     polyA_signal    9931..10052
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 poly(A) signal;SV40 polyadenylation signal"
     rep_origin      10071..10206
                     /label=SV40 ori
                     /note="SV40 ori"
                     /note="SV40 ori;SV40 origin of replication"
     promoter        complement(10239..10257)
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="T7 promoter;promoter for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(10267..10283)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="M13 fwd;common sequencing primer, one of multiple 
                     similar variants"
     rep_origin      10428..10883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct
     4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaggatc
     4741 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4801 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     4861 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4921 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     4981 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     5041 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5101 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5161 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5221 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     5281 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5341 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     5401 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5461 agctgtacaa gggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg
     5521 aggagaaccc tggacctgca ccaggaagta tggctcctcc taatctgtgg gcggcacagc
     5581 gttatgggcg tgaactgcgg cgtatggccg atgagggtga gggttccttc aagggtagcg
     5641 gaactgggtc tgggactggg agtggcaccg gttcagggac aggagatgtg tatcgctttg
     5701 ctgagcctga ctccgaagag aacattatct tcgaagagaa tatgcagcct aaagctggta
     5761 ttccgattat caaagccggg accgtgatta agctgatcga gcgtctgaca tatcacatgt
     5821 acgccgaccc caactttgtt cgcaccttcc tgaccacata tcgttccttt tgcaagccac
     5881 aggaactgct gtctctgatt atcgagcggt tcgaaattcc agaaccagag ccaaccgagg
     5941 cagatcgtat tgcgatcgaa aacggcgacc agcctctgag cgcggagctg aaacgttttc
     6001 ggaaggaata catccagccg gtccagctgc gggttctgaa tgtctgtcgc cattgggtgg
     6061 agcaccattt ttatgatttc gaacgggacg cttacctgct gcagcgcatg gaagagttca
     6121 ttggtacagt ccgcgggaaa gccatgaaaa agtgggtgga atccattact aagattatcc
     6181 agcgcaaaaa gatcgcccgt gataacgggc caggacacaa tatcacattt cagagctccc
     6241 cgcccactgt ggagtggcac atttctcgtc ccggtcatat cgaaaccttc gatctgctga
     6301 cactgcatcc aattgagatc gctcgtcagc tgacactgct ggaatctgac ctgtatcgtg
     6361 cagtgcagcc aagcgagctg gtgggctccg tttggaccaa agaggataag gaaattaaca
     6421 gtccaaatct gctgaaaatg atccgccaca ctaccaacct gactctgtgg tttgaaaagt
     6481 gcattgttga aaccgagaat ctggaagagc gtgtcgcggt ggttagccgg attatcgaga
     6541 tcctgcaggt gtttcaggaa ctgaacaatt tcaacggcgt tctggaggtc gtgtccgcaa
     6601 tgaacagcag ccctgtgtat cgcctggacc ataccttcga acagattccg agccgtcaga
     6661 aaaagatcct ggaagaggcg cacgagctgt ccgaagatca ttataaaaag tacctggcaa
     6721 aactgcgctc tattaaccca ccttgcgtgc cgtttttcgg tatttacctg actaacatcc
     6781 tgaagaccga agagggaaat ccggaggttc tgaaacgtca cggcaaggaa ctgatcaact
     6841 tttcaaaacg ccgtaaggtc gccgagattc tcggtgaaat ccagcagtat cagaatcagc
     6901 cttactgtct gcgtgtggag agcgacatta aacggttttt cgaaaacctg aatccgatgg
     6961 ggaacagtat ggaaaaagag tttaccgatt acctgttcaa taagtcactg gaaatcgagc
     7021 ctggctctgg ttcacagagc aaccgtgaac tggttgtcga ttttctgtcc tataaactgt
     7081 ctcagaaggg ctactcctgg tctcagttca gcgacgtcga agagaatcgg acagaggctc
     7141 cggaaggtac tgaatctgag atggaaaccc ccagtgccat taacgggaat ccatcatggc
     7201 acctggcaga tagccccgct gttaatggag ccaccggaca ttcgagctcc ctggacgccc
     7261 gcgaggttat cccaatggcc gcagtcaaac aggcgctgcg tgaagcagga gatgagtttg
     7321 aactgcgcta tcggcgcgcg ttcagtgacc tgacctcaca gctgcacatt acacccggca
     7381 ctgcatacca gagttttgag caggtggtta acgaactgtt ccgcgacgga gtgaattggg
     7441 gccgtatcgt tgcgtttttc agttttggcg gtgcactgtg tgtcgagtca gtggataaag
     7501 aaatgcaggt cctggtgtct cgtattgcag cttggatggc aacttatctg aacgatcatc
     7561 tggagccgtg gattcaggaa aatgggggat gggacacctt cgtggaactg tacggcaaca
     7621 atgcggcggc tgagagtcgt aaggggcagg agcggttcaa tcgttggttt ctgcagcaca
     7681 agctgcggaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
     7741 tgctctcctg aagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     7801 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7861 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7921 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7981 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8041 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     8101 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     8161 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     8221 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     8281 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     8341 gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     8401 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     8461 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     8521 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     8581 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     8641 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     8701 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     8761 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     8821 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     8881 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     8941 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     9001 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     9061 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     9121 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     9181 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     9241 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     9301 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     9361 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     9421 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     9481 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     9541 catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     9601 cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat
     9661 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9721 ggctgcctag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     9781 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     9841 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     9901 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     9961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
    10081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10141 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10201 gaggcctagg cttttgcgtc gagacgtacc caattcgccc tatagtgagt cgtattacgc
    10261 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    10321 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    10381 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag
    10441 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    10501 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    10561 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    10621 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    10681 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10741 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    10801 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
    10861 caaaatatta acgtttacaa tttcc
//
