LOCUS       Exported                5665 bp ds-DNA     circular SYN 23-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSL10-6xHA-LoxP-HX-LoxP-T7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Shaojun Long
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 14, 2016 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    7..40
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    41..1974
                     /note="HX"
     protein_bind    1975..2008
                     /bound_moiety="Cre recombinase"
                     /note="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(2053..2071)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2078..2094)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2236..2691
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2717..2821
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2822..3682
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3853..4441
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4765..4795
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4803..4819
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4827..4843
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4864..4882
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             4958..5212
                     /codon_start=1
                     /note="Feature 21"
                     /translation="ASKGSGSTQLYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAGLIKS
                     GRAGSGGGGGGSYPYDVPDYAGYPYDVPDYAYPYDVPDYA"
     CDS             4958..4963
                     /codon_start=1
                     /note="Feature 20"
                     /translation="AS"
     misc_feature    4964..4987
                     /note="Feature 19"
     CDS             4988..5077
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /note="3xHA"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5129..5155
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             5159..5185
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             5186..5212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    5222..5641
                     /note="HX 3'"
ORIGIN
        1 aagcttataa cttcgtatag catacattat acgaagttat gatcagcacg aaaccttgca
       61 ttcaaacccg cccgcggaag atccgatctt gctgctgttc gcagtcccag tagcgtcctg
      121 tcggccgcgc cgtctctgtt ggtgggcagc cgctacacct gttatctgac tgccgtgcgc
      181 gaaaatgacg ccatttttgg gaaaatcggg gaacttcatt ctttaaaagt atgcggaggt
      241 ttcctttttc ttctgttcgt ttctttttct cgggtttgat aaccgtgttc gatgtaagca
      301 ctttccgtct ctcctccgtg ctttgttcga catcgagacc aggtgtgcag atccttcgct
      361 tgtcgatccg gagacgcgtg tctcgtagaa ccttttcatt ttaccacacg gcagtgcgga
      421 gcactgctct gagtgcagca gggacgggtg aagtttcgct ttagtagtgc gtttctgctc
      481 tacggggcgt tgtcagatcc aaaatggcgt ccaaacccat tgaagactac ggcaagggca
      541 agggccgtat tgagcccatg tatatccccg acaacacctt ctacaacgct gatgactttc
      601 ttgtgccccc ccactgcaag ccctacattg acaaaatcct cctccctggt ggattggtca
      661 aggacagagt tgagaagttg gcgtatgaca tccacagaac ttacttcggc gaggagttgc
      721 acatcatttg catcctgaaa ggctctcgcg gcttcttcaa ccttctgatc gactaccttg
      781 ccaccataca gaagtacagt ggtcgtgagt ccagcgtgcc ccccttcttc gagcactatg
      841 tccgcctgaa gtcctaccag aacgacaaca gcacaggcca gctcaccgtc ttgagcgacg
      901 acttgtcaat ctttcgcgac aagcacgttc tgattgttga ggacatcgtc gacaccggtt
      961 tcaccctcac cgagttcggt gagcgcctga aagccgtcgg tcccaagtcg atgagaatcg
     1021 ccaccctcgt cgagaagcgc acagatcgct ccaacagctt gaagggcgac ttcgtcggct
     1081 tcagcattga agacgtctgg atcgttggtt gctgctacga cttcaacgag atgttccgcg
     1141 acttcgacca cgtcgccgtc ctgagcgacg ccgctcgcaa aaagttcgag aagtaaaccc
     1201 tgcatagccc tacagaagct gcccgtctct cgttttcctc tcttttcgga gggatcaggg
     1261 agagtgcctc gggtcggaga gagctgacga gggggtgcca gagacccctg tgtcctttat
     1321 cgaagaaaag ggatgactct tcatgtggca tttcacacag tctcacctcg ccttgttttc
     1381 tttttgtcaa tcagaacgaa agcgagttgc gggtgacgca gatgtgcgtg tatccactcg
     1441 tgaatgcgtt atcgttctgt atgccgctag agtgctggac tgttgctgtc tgcccacgac
     1501 agcagacaac tttccttcta tgcacttgca ggatggtgca gcgcaaacga cggagagaaa
     1561 ggagcaccct ctcagtttcc ctacgatgtg ctgtcagttt cgactcttca ccgcgaacga
     1621 ttggcgatac gtctctgttg acttgttagg ctccgaccac gaagctccct taactaaata
     1681 agccgcgaca cctaagtgta caccatttgc agatcgataa tctgcgaccg ctgaatccgt
     1741 ccagatcagt aaaaccgcac cacctaagtg taaaccttgt ttaggtcgat aaaatgctac
     1801 caacccccac ccacaatcga gccttgagcg tttctgcgca cgcgttggcc tacgtgactt
     1861 gctgatgcct gcctctggcc attcatgcca gtcagtgcgc ataaaaatgt ggacacagtc
     1921 ggttgacaag tgttctggca ggctacagtg acaccgcggt ggagggggat ccacataact
     1981 tcgtatagca tacattatac gaagttatct gcagtctaga gcggccgcca ccgcggtgga
     2041 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     2101 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccccttcg
     2161 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgtagcc
     2221 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2281 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2341 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2401 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2461 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     2521 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     2581 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2641 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt taggtggcac
     2701 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2761 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2821 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2881 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2941 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     3001 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     3061 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     3121 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     3181 atgcagtgct gccataagca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     3241 cggaggaccg aaggagctaa ccgctttttt tcacaacatg ggggatcatg taactcgcct
     3301 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3361 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3421 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3481 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3541 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3601 cacgacgggc agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3661 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3721 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3781 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3841 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3901 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3961 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4021 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4081 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4141 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4201 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     4261 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4321 gcgcacgagg gagcttccag gggggaacgc ctggtatctt tatagtcctg tcgggtttcg
     4381 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggccga gcctatggaa
     4441 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4501 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4561 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4621 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     4681 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     4741 cctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta tgttgtgtgg
     4801 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     4861 cggaattaac cctcactaaa gggaacaaaa gctgggtacc ctgtacttcc aatccaattt
     4921 aattaaaatt ggaagtggag gacgggaatt cgggcccgct agcaagggct cgggctcgac
     4981 ccagctgtac ccatacgatg ttccagatta cgctggctat ccctatgacg tcccggacta
     5041 tgcaggatcc tatccatatg acgttccaga ttacgctgga ttaattaaat cggggcgcgc
     5101 cggctctggc ggcggcggcg gcggctctta cccgtacgac gtcccggact acgctggcta
     5161 tccctatgat gtgcccgatt atgcgtatcc ttacgatgtt ccagattatg cctaacccgg
     5221 gcatatgtag aaaagttgta acgttagtaa acgtaacaca cgggtataat gccatttaat
     5281 ggcgccgtat atttttcact ccccctctcc gataggagga aaaagacgat ccgtctgcga
     5341 atgtatatta catcgaccgg ccatagtacg agaagtggtg ccggtattgt tctgtccggg
     5401 ggatgtacca tcctattttt tgtatatgtc gttcttgtcg gggagcaaca gcccatcttc
     5461 cggctattgt ctcgtatcag ttgtgaaaca gactgcgttt tgccgaaaat gagtcctatg
     5521 tccgatggct atttgtaccg cttcgtacca tttcgcttgt cagctgtaat caaccgaatt
     5581 catttggcga gacatgattc cgattaaatt ctttactgcg cgtgctcgga tccactagtt
     5641 ctactcgagg gtcgacggta tcgat
//
