LOCUS       Exported                5840 bp ds-DNA     circular SYN 26-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRN3p Suv4-20h1 WT
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 26, 2017 from SnapGene 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        2..20
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           45..87
                     /note="Xenopus globin 5'-UTR"
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     3'UTR           2814..2939
                     /note="Xenopus globin 3'-UTR"
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(3028..3046)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3053..3069)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3211..3666)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3695..3799
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             3800..4660
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4831..5419
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5707..5728
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5743..5773
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5781..5797
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5805..5821
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaattaaccc tcactaaagg gaacaaaagc tggagctaag cttgcttgtt ctttttgcag
       61 aagctcagaa taaacgctca actttggcag atctgccgcc atgggctacc catacgatgt
      121 tcctgactat gcgggatcca tgaagtggtt gggagactcc aagaacatgg tggtgaatgg
      181 caggagaaat ggaggcaagt tgtctaatga ccatcagcag aatcaatcaa aattacagca
      241 gcactcgggc aaggacaccc tgaagaccgg cagaaacgcc gttgagaggc ggtccagcag
      301 atgtcatggt aactcgggat ttgaagggca gagccgctat gtgccgtcct ctggaatgtc
      361 cgccaaggag ctctgtgaga acgatgactt agcaaccagt ttggttcttg atccctactt
      421 aggttttcag acacacaaaa tgaacactag cgcctttcct tcgaggagct cgaggcatat
      481 ttcaaaagct gacagttttt ctcacaacaa tcctgtgaga tttcggccta taaaaggaag
      541 gcaagaagag ctaaaggaag taattgaacg ctttaagaaa gatgaacact tagagaaagc
      601 tttcaaatgt ttgacttctg gggaatgggc acggcattat tttctcaaca aaaacaaaat
      661 gcaggagaaa ttattcaagg aacatgtctt tatttacttg cggatgtttg caactgacag
      721 tggatttgaa atactgcctt gtaatagata ttcttcagaa caaaatggag ccaagatagt
      781 tgcaacaaaa gagtggaaac gaaatgacaa aatagaatta ctggtgggtt gtattgccga
      841 actttcagaa attgaggaga acatgctact tagacacgga gaaaacgact tcagtgtcat
      901 gtattccaca aggaaaaatt gtgctcaact ctggctcggt cctgctgcat ttataaatca
      961 tgattgcaga cctaactgta agtttgtgtc aactggtcga gatacagcat gcgttaaggc
     1021 tctgagagat attgaacctg gagaagaaat ttcttgttac tatggagatg gcttttttgg
     1081 agaaaataat gagttctgcg aatgttatac ttgtgaaaga cggggaactg gtgcttttaa
     1141 atcacgagta ggactgcctg cgcctgctcc tgttatcaat agcaaatacg gacttagaga
     1201 aacagataaa cgcttaaata ggcttaaaaa gttaggtgac agcagcaaaa actcagacag
     1261 tcagtctgtc agctctaaca cagatgcaga caccactcag gaaaaagaca atgcaacttc
     1321 taatcgaaaa tcttcagttg gtgtgaaaaa gagcagcaag agtcgagctc tgacaaggcc
     1381 gtccatgccg agagtcccgg ctgcttccaa ctctacctca cccaagctag tgcacaccaa
     1441 caatccccgg gtaccaaaga aactgagaaa gccggcaaag cctttactct ccaagatcag
     1501 actgcggaat cactgcaagc ggctggacca gaagagcgca tcccgcaagc tcgagatggg
     1561 gagcttagtg cttaaggagc ccaaagtcgt gctatataaa aatttgccaa ttaagaaaga
     1621 aagggagcca gagggaccag cccatgctgc agtggggagt gggtgcttga ctagacatgc
     1681 tgcgagagaa cacaggcaga atcatgggag aggtgctcat tcgcagggcg acagtttgcc
     1741 ctgcacctac acaacccggc gctctttgag gacaaggaca ggtctgaagg agaccactga
     1801 catcaagctt gaaccaagtc ccttggatgg ctataaaaat ggtatactgg aaccttgccc
     1861 agacagtggc cagcagccaa ccccagaggt gctggaagaa ctggctcctg agactgcaca
     1921 cagggaggaa gcatcccagg agtgtcccaa gaacgactcc tgcctgtcac gaaagaaatt
     1981 tcgacaagtg aaacctgtga aacacttagc aaagaccgag gactgcagtc cagagcacag
     2041 cttccctggg aaagacgggc tgccagattt gccagggtct catcctgatc aaggtgagcc
     2101 cagtggcaca gtcagggtgc ccgtgagcca cacggactct gctccctcac cggttggctg
     2161 ctctgttgtc gcacccgaca gcttcacaaa agacagcttc agaactgcac aaagtaaaaa
     2221 gaagcggcgg gtcaccaggt acgatgcaca gctgatcctg gagaacagct ctggaatccc
     2281 caagctgacg cttcgcaggc ggcacgacag cagcagcaag acaaacgacc atgagagtga
     2341 cggcgtgaac tcctccaaga tcagcatcaa actcagcaag gaccacgaca gtgacagcaa
     2401 cctctatgtt gccaagctca gtaacggggt cagcgcaggg ccgggcagca gctccaccaa
     2461 gctcaagatc cagctcaagc gggatgagga gagcaggggg ccatgtgcag agggcctgca
     2521 cgagaacggg gtgtgctgca gcgaccccct ctccctgctc gagtcccaga tggaggtgga
     2581 cgactacagt caatatgagg aggacagcac agatgaatcc tcatcttctg agggggagga
     2641 ggaggaggag gactgcgagg atgacttcga tgatgacttc attcctcttc ctccggcaaa
     2701 gcggctgagg ctaattgttg gtaaagactc catagatatt gacatttctt caaggagaag
     2761 agaagatcag tctctaagac tgaacgcagc ggccgcggat ctggttacca ctaaaccagc
     2821 ctcaagaaca cccgaatgga gtctctaagc tacataatac caacttacac tttacaaaat
     2881 gttgtccccc aaaatgtagc cattcgtatc tgctcctaat aaaaagaaag tttcttcaca
     2941 ttctaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaccc cccccccccc ccccctgcag
     3001 ggccaagtcg gccggtaccc aattcgccct atagtgagtc gtattacaat tcactggccg
     3061 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3121 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3181 aacagttgcg cagcctgaat ggcgaagggc aaattgtaag cgttaatatt ttgttaaaat
     3241 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3301 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3361 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3421 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3481 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3541 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3601 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3661 gcgcgtccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3721 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3781 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3841 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3901 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3961 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4021 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4081 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4141 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4201 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4261 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4321 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4381 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4441 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4501 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4561 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4621 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4681 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4741 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4801 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4861 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4921 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     4981 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5041 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5101 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5161 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5221 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5281 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5341 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5401 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5461 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5521 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5581 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5641 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5701 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     5761 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     5821 ccatgattac gccaagctcg
//
