LOCUS       Exported                4610 bp ds-DNA     circular SYN 25-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRN3p-Suv4-20h2mut
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 26, 2017 from SnapGene 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        2..20
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           45..87
                     /note="Xenopus globin 5'-UTR"
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     primer_bind     complement(135..151)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     3'UTR           1584..1709
                     /note="Xenopus globin 3'-UTR"
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(1798..1816)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1823..1839)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1981..2436)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2465..2569
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2570..3430
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3601..4189
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4477..4498
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4513..4543
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4551..4567
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4575..4591
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaattaaccc tcactaaagg gaacaaaagc tggagctaag cttgcttgtt ctttttgcag
       61 aagctcagaa taaacgctca actttggcag atctgccgcc atgggctacc catacgatgt
      121 tcctgactat gcgggatcca ctagttctag aatggggcct gatcgagtga cagcccgaga
      181 actgtgcgag aatgatgacc tggccaccag ccttgttctt gacccctacc tcggcttccg
      241 cacccataag atgaatgtca gtcctgtgcc caccttgcga cgacaacacc accttcgttc
      301 agcactggaa gctttcctaa ggcaacggga cctggaggct gcattccggg ccctgacgct
      361 aggaggctgg atggcccact acttccagag ccgtgcccct cggcaggagg ctgctctcaa
      421 gacccacatt ttctgctacc tccgcgcctt cctgcctgag agtggcttca ccattctgcc
      481 ctgcacccgc tactccatgg agaccaatgg ggccaagatt gtgtctaccc gtgcttggaa
      541 gaagaatgag aagctggagc tgctggtggg ctgcattgca gagctgcgtg aagaggatga
      601 agacctgctt agggccggtg agaacgactt cagcatcatg tactccaccc gaaaaaggag
      661 tgcccagctg tggcttggcc cagctgcctt catcgccgct gccggcaaac ccaactgcaa
      721 gtttgtgccc tcagatggga atactgcctg tgtgaaggtg ctacgggaca ttgagccagg
      781 ggatgaagtg acttgcttct atggtgaggg cttcttcggc gagaagaatg agcactgtga
      841 atgctatacc tgtgagagga aaggcgaggg agctttcaga cttcagccca gggagcctga
      901 gctgcgacca aagcccctag acaagtatga gctccgagag acaaagaggc gtctgcagca
      961 gggcttggtc agcagccagc agagcctgat gagccggtgg gcctgctccc acctgtcccc
     1021 actgcgccct gacccattct gtgctgcctg ccagccctca tgcctgctgc ctgccagccc
     1081 ccacatggat tacttgcccc tttggctcca gcgggcgcct caaccccagc ccatagtacc
     1141 tcccaggaag cgccaccgcc gccgccgccc caggatccgc caggcttcat tgcctcctgt
     1201 cctccgcact gcctgtgtcc ccctgcaccg atggggaggc tgtggtcccc actgccagct
     1261 acgtgctgag gccatggtga ccctacacct gcgcccccag actcgctgga ccccacaaca
     1321 ggactggtac tgggcccggc gctatgggct accttctgtg gggcgtgtgg aacttacccg
     1381 cctagcccca gccctgccag ctgctcctgc ccctgctggg aacccaggtc ctgtccccac
     1441 tcctgatttc atccctaagc aggcccttgc ctttgctccg ttctgcccac ctaagcgcct
     1501 ccggctagtg gtcagtcatg gttctattga tctggacatc aatagtggtg agccataagc
     1561 ggccgcggat ctggttacca ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc
     1621 tacataatac caacttacac tttacaaaat gttgtccccc aaaatgtagc cattcgtatc
     1681 tgctcctaat aaaaagaaag tttcttcaca ttctaaaaaa aaaaaaaaaa aaaaaaaaaa
     1741 aaaaaaaccc cccccccccc ccccctgcag ggccaagtcg gccggtaccc aattcgccct
     1801 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     1861 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     1921 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaagggc
     1981 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     2041 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     2101 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     2161 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     2221 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2281 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     2341 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     2401 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ggtggcactt ttcggggaaa
     2461 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2521 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2581 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2641 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2701 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2761 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2821 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2881 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2941 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3001 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3061 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3121 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3181 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3241 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3301 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3361 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3421 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3721 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4261 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4321 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4381 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4441 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4501 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4561 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg
//
