LOCUS       Exported                9487 bp ds-DNA     circular SYN 04-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p-sOPTiKD
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   A Bertero
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 19, 2016 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        143..173
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        242..260
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /note="AAVS1-left"
                     /note="'left homology arm to AAVS1"""
     misc_feature    1105..1130
                     /note="homology"
                     /note="region of homology between this vector and the 
                     portion of the OCT4 GFP puro donor 1 vector which should 
                     carry the sA sequence"
     misc_feature    1118..1130
                     /note="sA"
                     /note="PUTATIVE 3' splice acceptor sequence (I predicted it
                     from online tools and by analogy with other plasmids, but I
                     have not 100% certainty"
     CDS             1130..1816
                     /codon_start=1
                     /note="SA-2A-puro"
                     /translation="GLESSGSGEGRGSLLTCGDVEENPGPRLEMTEYKPTVRLATRDDV
                     PRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAV
                     WTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDH
                     QGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCM
                     TRKPGA"
     CDS             1154..1207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1217..1816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    1854..2078
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2086..2305
                     /note="H1 promoter"
     misc_feature    2310
                     /note="+1"
                     /note="Start of transcription of RNA"
     enhancer        2396..2699
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2701..2978
                     /note="chicken beta-actin promoter"
     CDS             4065..4715
                     /codon_start=1
                     /note="OPT-tetR-nls"
                     /translation="MPKKKRKVSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQ
                     PTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGA
                     KVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKE
                     ERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSPR"
     CDS             4068..4088
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    4742..4853
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4984..5820
                     /note="AAVS1-right"
                     /note="right homology arm of AAVS1"
     promoter        complement(5860..5878)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6039..6341
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             6690..7484
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7734..8594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8718..9306
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag
     1141 ttctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     1201 cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     1261 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     1321 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     1381 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     1441 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     1501 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     1561 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     1621 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     1681 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     1741 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     1801 caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     1861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2041 caatagcagg catgctgggg atgcggtggg ctctatgggt caattcgaac gctgacgtca
     2101 tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt
     2161 gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat
     2221 gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt
     2281 ataagttccc tatcagtgat agagatctaa gcttatcgat accgtcgacc tcgactagtt
     2341 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     2401 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     2461 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     2521 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     2581 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     2641 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     2701 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     2761 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
     2821 ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg
     2881 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
     2941 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg gagtcgctgc gacgctgcct
     3001 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     3061 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     3121 ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
     3181 gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
     3241 cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     3301 ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg
     3361 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
     3421 gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga
     3481 gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc
     3541 gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga
     3601 gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag
     3661 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
     3721 aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc
     3781 gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg
     3841 ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg
     3901 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     3961 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     4021 tgtgctgtct catcattttg gcaaagaatt aattcggatc caccatgccc aagaaaaagc
     4081 ggaaggtgtc ccggctggac aagagcaaag tgatcaacag cgccctggaa ctgctgaacg
     4141 aagtgggcat cgagggcctg accacccgga agctggccca gaaactgggc gtggaacagc
     4201 ccaccctgta ctggcacgtg aagaacaagc gggccctgct ggacgccctg gccatcgaga
     4261 tgctggaccg gcaccacaca cacttttgcc ccctggaagg cgaaagctgg caggacttcc
     4321 tgcggaacaa cgccaagagc ttcagatgcg ccctgctgag ccaccgggac ggcgccaaag
     4381 tgcacctggg caccagaccc accgagaagc agtacgagac actggaaaac cagctggcct
     4441 tcctgtgcca gcagggcttc agcctggaaa acgccctgta cgccctgagc gccgtgggcc
     4501 actttaccct gggctgcgtg ctggaagatc aggaacacca ggtcgccaaa gaggaaagag
     4561 agacacccac caccgacagc atgccccccc tgctgagaca ggccatcgag ctgttcgatc
     4621 atcaaggcgc cgagcccgcc ttcctgttcg gcctggaact gatcatctgc ggcctcgaga
     4681 agcagctgaa gtgcgagagc ggctccccca gatgaacgcg tagctcgctg atcagcctcg
     4741 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4801 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4861 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4921 tgggaagaga atagcaggca tgctgggggt ctcgaggggg ggcccggaca gtactaagct
     4981 ttactaggga caggattggt gacagaaaag ccccatcctt aggcctcctc cttcctagtc
     5041 tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct ggtgacacac
     5101 ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc ttacgatgga
     5161 gccagagagg atcctgggag ggagagcttg gcagggggtg ggagggaagg gggggatgcg
     5221 tgacctgccc ggttctcagt ggccaccctg cgctaccctc tcccagaacc tgagctgctc
     5281 tgacgcggct gtctggtgcg tttcactgat cctggtgctg cagcttcctt acacttccca
     5341 agaggagaag cagtttggaa aaacaaaatc agaataagtt ggtcctgagt tctaactttg
     5401 gctcttcacc tttctagtcc ccaatttata ttgttcctcc gtgcgtcagt tttacctgtg
     5461 agataaggcc agtagccagc cccgtcctgg cagggctgtg gtgaggaggg gggtgtccgt
     5521 gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt tcaccaggtc gtggccgcct
     5581 ctactccctt tctctttctc catccttctt tccttaaaga gtccccagtg ctatctggga
     5641 catattcctc cgcccagagc agggtcccgc ttccctaagg ccctgctctg ggcttctggg
     5701 tttgagtcct tggcaagccc aggagaggcg ctcaggcttc cctgtccccc ttcctcgtcc
     5761 accatctcat gcccctggct ctcctgcccc ttccctacag gggttcctgg ctctgctcta
     5821 agggcaaggg cgaattcgcg gccgctaaat tcaattcgcc ctatagtgag tcgtattaca
     5881 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5941 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6001 atcgcccttc ccaacagttg cgcagcctat acgtacggca gtttaaggtt tacacctata
     6061 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgccgg
     6121 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
     6181 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
     6241 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
     6301 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc atgagattat
     6361 caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct
     6421 gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga
     6481 gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga
     6541 cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca
     6601 aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagctctg
     6661 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6721 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6781 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6841 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     6901 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6961 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7021 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7081 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7141 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7201 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7261 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7321 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7381 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7441 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattatt aacgcttaca
     7501 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatcaggt
     7561 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7621 aatatgtatc cgctcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8641 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8701 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8761 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8821 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8881 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8941 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     9001 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9061 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9121 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9181 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9241 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9301 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9361 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9421 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9481 gcggaag
//
