LOCUS       Exported                9585 bp ds-DNA     circular SYN 23-FEB-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    p-sOPTiKO-TO
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   J.D.Cooper
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 19, 2016 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     promoter        143..173
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     promoter        242..260
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /note="AAVS1-left"
                     /note="'left homology arm to AAVS1"""
     misc_feature    1105..1130
                     /note="homology"
                     /note="region of homology between this vector and the 
                     portion of the OCT4 GFP puro donor 1 vector which should 
                     carry the sA sequence"
     misc_feature    1118..1130
                     /note="sA"
                     /note="PUTATIVE 3' splice acceptor sequence (I predicted it
                     from online tools and by analogy with other plasmids, but I
                     have not 100% certainty"
     CDS             1130..1816
                     /codon_start=1
                     /note="SA-2A-puro"
                     /translation="GLESSGSGEGRGSLLTCGDVEENPGPRLEMTEYKPTVRLATRDDV
                     PRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAV
                     WTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDH
                     QGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCM
                     TRKPGA"
     CDS             1154..1207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1217..1816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    1854..2078
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2086..2305
                     /note="H1 promoter"
     misc_RNA        2340..2415
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the CRISPR/Cas9 system"
     enhancer        2494..2797
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2799..3076
                     /note="chicken beta-actin promoter"
     misc_feature    3172..3645
                     /note="INTRON"
                     /note="chicken beta actin intro + rabbit beta globin 
                     intron"
     misc_feature    3734..4046
                     /note="INTRON"
                     /note="chicken beta actin intro + rabbit beta globin 
                     intron"
     CDS             4163..4813
                     /codon_start=1
                     /note="OPT-tetR-nls"
                     /translation="MPKKKRKVSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQ
                     PTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGA
                     KVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKE
                     ERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSPR"
     CDS             4166..4186
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    4840..4951
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5082..5918
                     /note="AAVS1-right"
                     /note="right homology arm of AAVS1"
     promoter        complement(5958..5976)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6137..6439
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             6788..7582
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7832..8692)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8816..9404
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag
     1141 ttctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     1201 cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     1261 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     1321 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     1381 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     1441 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     1501 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     1561 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     1621 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     1681 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     1741 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     1801 caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     1861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2041 caatagcagg catgctgggg atgcggtggg ctctatgggt caattcgaac gctgacgtca
     2101 tcaacccgct ccaaggaatc gcgggcccag tgtcactagg cgggaacacc cagcgcgcgt
     2161 gcgccctggc aggaagatgg ctgtgaggga caggggagtg gcgccctgca atatttgcat
     2221 gtcgctatgt gttctgggaa atcaccataa acgtgaaatg tctttggatt tgggaatctt
     2281 ataagttccc tatcagtgat agagatccct ggtgcaggtg gactgactca cctgcacctg
     2341 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     2401 gcaccgagtc ggtgcttttt tgtcgacctc gactagttat taatagtaat caattacggg
     2461 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2521 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2581 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2641 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2701 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2761 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     2821 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     2881 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
     2941 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
     3001 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     3061 aagcgcgcgg cgggcgggga gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc
     3121 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     3181 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     3241 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     3301 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     3361 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     3421 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     3481 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     3541 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     3601 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggctggtg
     3661 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     3721 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     3781 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
     3841 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     3901 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     3961 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     4021 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
     4081 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
     4141 aaagaattaa ttcggatcca ccatgcccaa gaaaaagcgg aaggtgtccc ggctggacaa
     4201 gagcaaagtg atcaacagcg ccctggaact gctgaacgaa gtgggcatcg agggcctgac
     4261 cacccggaag ctggcccaga aactgggcgt ggaacagccc accctgtact ggcacgtgaa
     4321 gaacaagcgg gccctgctgg acgccctggc catcgagatg ctggaccggc accacacaca
     4381 cttttgcccc ctggaaggcg aaagctggca ggacttcctg cggaacaacg ccaagagctt
     4441 cagatgcgcc ctgctgagcc accgggacgg cgccaaagtg cacctgggca ccagacccac
     4501 cgagaagcag tacgagacac tggaaaacca gctggccttc ctgtgccagc agggcttcag
     4561 cctggaaaac gccctgtacg ccctgagcgc cgtgggccac tttaccctgg gctgcgtgct
     4621 ggaagatcag gaacaccagg tcgccaaaga ggaaagagag acacccacca ccgacagcat
     4681 gccccccctg ctgagacagg ccatcgagct gttcgatcat caaggcgccg agcccgcctt
     4741 cctgttcggc ctggaactga tcatctgcgg cctcgagaag cagctgaagt gcgagagcgg
     4801 ctcccccaga tgaacgcgta gctcgctgat cagcctcgac tgtgccttct agttgccagc
     4861 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4921 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4981 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     5041 ctgggggtct cgaggggggg cccggacagt actaagcttt actagggaca ggattggtga
     5101 cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc
     5161 cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc
     5221 tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg
     5281 agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg
     5341 ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt
     5401 tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa
     5461 acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc
     5521 aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc
     5581 cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga
     5641 atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca
     5701 tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag
     5761 ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag
     5821 gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct
     5881 cctgcccctt ccctacaggg gttcctggct ctgctctaag ggcaagggcg aattcgcggc
     5941 cgctaaattc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     6001 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6061 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     6121 cagcctatac gtacggcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     6181 gtttgtggat gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct
     6241 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     6301 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     6361 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     6421 gatgttctgg ggaatataaa tgtcaggcat gagattatca aaaaggatct tcacctagat
     6481 ccttttcacg tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta
     6541 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
     6601 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
     6661 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
     6721 gccgccaagg atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc
     6781 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     6841 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     6901 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6961 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     7021 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     7081 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     7141 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     7201 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     7261 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     7321 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     7381 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     7441 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     7501 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7561 ccttcttgac gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct
     7621 ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc
     7681 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagat
     7741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8701 tttttcaata ttattgaagc atttatcagg gttattgtct catgaccaaa atcccttaac
     8761 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8821 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8881 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8941 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9001 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9061 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9121 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9181 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9241 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9301 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9361 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9421 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9481 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9541 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
