LOCUS       Exported                7526 bp ds-DNA     circular SYN 20-MAR-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 11
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    236..852
                     /label=CMV immearly promoter
                     /note="CMV immearly promoter"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    315..602
                     /label=CAG enhancer
                     /note="CAG enhancer"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    995..1000
                     /label=HindIII
                     /note="HindIII"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=#ff0000"
                     /note="color: #ff0000"
     CDS             1020..3074
                     /codon_start=1
                     /note="lincSLC39A11"
                     /note="ApEinfo_revcolor=pink vntifkey=4 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=pink"
                     /note="color: #993366"
                     /translation="RARRRRGCAVRRWYPEDAVTASGICRSALLRKDPLLGRRPP*ASR
                     AL*TCKPKSERRSAFSAFQLGREAFPAGIFGWRWRCAGKKGEERRPGWVMSQHE*AGCA
                     RLVLRAPGPCPRRAPTPGDEKAAA*GPPPATSASLERRGQGLAALWPWMESGELGGGLR
                     TP*RGLLGVYQLGSEDPRPLLHPWIDLFCLTAGSPSWRYFSLSTLVFSCNVWPPFPARP
                     PAFLVHFLENVAEHGASCL*SQHFGRPVWEDLLSPGVQD*PGQHRETPSLK*****NKK
                     LAGHGGMCL*SQLPRRLRQEDGWSLGC*GCNEL*LPHCTPAWAKERETLSQIIIIIIIL
                     FWRIKRPLDLRCHLGRKKRRLHREIVCVALKEQRDASLEVTYS*RRLIMTDLVLLPCGK
                     CFLCCYCS*DSSPSLSQGTKGLSTTPFLAPRLPCLLCLCTQQFLFAPLSSSRIQSE*LT
                     TLRSNRI*IFVPAPCKEASVCVLQAALE**LFSFVWDLATKFQNDTRISAEVHQALGP*
                     GCWRRLQQQN*W*RLVFSILNFLCFDHTQEYIWKGKK*TLSFIAAVFCDTDAQSAVKTF
                     SQVAFGSPCQIMVLHERLTAIRGCGT**GLSSPSVC**HIHT*Q*LESSLFLLLCSQHT
                     HDLKPFLNIYHGSSLSKCRVRAICTSLLFPRRIVGFLFAKIIKVFVCCSC"
     misc_feature    3094..3099
                     /label=HindIII(1)
                     /note="HindIII(1)"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_label=HindIII 
                     ApEinfo_fwdcolor=#ff0000"
                     /note="color: #ff0000"
     misc_feature    3916..4140
                     /label=BGH polyA signal
                     /note="BGH polyA signal"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=#ff80c0"
                     /note="color: #ff80c0"
     misc_feature    4185..4614
                     /label=F1 origin
                     /note="F1 origin"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=#80ff80"
                     /note="color: #80ff80"
     misc_feature    4234..5028
                     /label=Neo/Kan
                     /note="Neo/Kan"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=#ffff00"
                     /note="color: #ffff00"
     misc_feature    5759..6375
                     /label=Ori
                     /note="Ori"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=#80ffff"
                     /note="color: #80ffff"
     misc_feature    6530..7390
                     /label=Amp
                     /note="Amp"
                     /note="ApEinfo_revcolor=green 
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0 ApEinfo_fwdcolor=#ce5831"
                     /note="color: #ce5831"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
      961 accacactgg actagtggat ccgagctcgg taccaagctt cccgggatac ttaggatcca
     1021 gggcgcgcag gcggcgcggg tgcgcggtgc ggcgctggta tccagaggac gcggtcaccg
     1081 cctctggcat ttgtcgttct gcgcttctcc gcaaggaccc tctgttaggc aggcgcccac
     1141 cgtaagcctc ccgggccttg tgaacctgca aacccaagtc tgagagacga tccgccttca
     1201 gcgctttcca gcttggcaga gaggctttcc cggcggggat ctttggttgg cgctggcgat
     1261 gcgcggggaa gaaaggcgag gagcggcgtc caggctgggt gatgtcccag cacgagtagg
     1321 cgggatgcgc tcgcttggtc ctccgggcgc ccggtccctg cccgcgtcgc gcgcccaccc
     1381 ctggggacga gaaggcggcc gcctgaggac ccccgcccgc gacctccgcg agtctggagc
     1441 gcagaggaca gggtctggct gctctttggc cttggatgga aagtggggaa ttgggtgggg
     1501 ggctgcggac cccttaacgt ggattacttg gtgtgtatca gctgggctca gaagacccac
     1561 gacctcttct ccatccgtgg attgatttgt tctgcttaac agctgggtcg ccaagctgga
     1621 ggtatttttc cctctccacc ctggtcttct cctgtaacgt gtggccgcct tttccagcac
     1681 ggcctcctgc cttcctggtg cactttttgg agaacgtggc tgagcatggt gcctcatgcc
     1741 tataatccca acactttggg aggccagtgt gggaggatct cttgagccca ggagttcaag
     1801 actagcctgg gcaacacaga gagaccccat ctctaaaata ataataataa taaaataaaa
     1861 aattagcagg gcatggtggc atgtgcctgt agtcccagct acccaggagg ctgaggcaag
     1921 aggatggctg gagcctggga tgttgaggct gcaatgaact gtgattaccc cactgcactc
     1981 cagcctgggc aaaagagcga gagaccctgt ctcaaataat aataataata ataatcttat
     2041 tttggagaat aaagagacct ctggatttga ggtgccattt gggtagaaag aaaagacgtt
     2101 tacaccgaga aatagtctgt gttgccctga aggagcagag ggatgcatcg ctggaggtga
     2161 cctacagttg aagaagactc attatgacag accttgtcct tcttccttgt ggaaagtgtt
     2221 tcctctgctg ctactgctca tgagactctt ccccctccct gtcccaggga accaaagggc
     2281 tttctaccac accctttctt gccccccgcc tcccatgtct gctgtgcctt tgtactcagc
     2341 aattcttgtt tgctccatta tcttccagcc ggatacagag tgaatagtta accacactta
     2401 ggtcaaatag gatctaaatt tttgttcctg ctccgtgtaa agaggccagt gtttgtgtgt
     2461 tgcaagcagc cttggaatag taactcttct catttgtttg ggatctggcc accaagttcc
     2521 agaatgatac acggatcagt gcagaagttc atcaggctct cggaccttag ggctgttgga
     2581 gaaggcttca gcagcagaac tgatggtgaa ggctcgtgtt ctccatcctc aactttcttt
     2641 gcttcgatca tacacaagaa tacatttgga agggcaaaaa atgaacactg tcgttcattg
     2701 cagccgtgtt ttgtgacaca gatgcacagt ctgctgtgaa gaccttctct caagtggcat
     2761 ttgggagtcc atgccagatc atggtgcttc atgagagact gacagctatc aggggttgtg
     2821 gcacttagtg aggactctcc tcccccagtg tgtgctgatg acacatacac acctgacaat
     2881 agcttgagtc ttctctgttc cttttactct gtagccaaca tacacatgat ttaaaaccct
     2941 ttctaaatat ctatcatggt tcatccttgt ccaaatgcag agtcagagct atttgtactt
     3001 cattattatt tccaaggcga atagttggct ttctttttgc aaaaataatt aaagtttttg
     3061 tatgttgcag ttgctctaga taagtatcac gtgaagctta agtttaaacc gctgatcagc
     3121 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3181 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3241 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3301 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     3361 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     3421 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3481 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3541 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3601 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3661 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3721 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3781 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     3841 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3901 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3961 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4021 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4081 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4141 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4201 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4261 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4321 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4381 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     4441 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4501 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4561 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4621 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4681 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4741 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     4801 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4861 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4921 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4981 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5041 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5101 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5161 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5221 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5281 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5341 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5581 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5641 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5701 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5761 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5821 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5881 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5941 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6001 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6061 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6121 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6181 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6241 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6301 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6361 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6421 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6481 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6541 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6601 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6661 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6721 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6781 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6841 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6901 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6961 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7021 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7081 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7141 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7201 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7261 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7321 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7381 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7441 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7501 ttccccgaaa agtgccacct gacgtc
//
