LOCUS       Exported                7567 bp ds-DNA     circular SYN 20-MAR-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    236..852
                     /label=CMV immearly promoter
                     /note="CMV immearly promoter"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    315..602
                     /label=CAG enhancer
                     /note="CAG enhancer"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    995..1000
                     /label=HindIII
                     /note="HindIII"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff0000"
                     /note="color: #ff0000"
     CDS             1020..3115
                     /codon_start=1
                     /note="lincSLC39A11"
                     /note="ApEinfo_revcolor=pink
                     vntifkey=4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #993366"
                     /translation="RARRRRGCAVRRWYPEDAVTASGICRSALLRKDPLLGRRPP*ASR
                     AL*TCKPKSERRSAFSAFQLGREAFPAGIFGWRWRCAGKKGEERRPGWVMSQHE*AGCA
                     RLVLRAPGPCPRRAPTPGDEKAAA*GPPPATSASLERRGQGLAALWPWMESGELGGGLR
                     TP*RGLLGVYQLGSEDPRPLLHPWIDLFCLTAGSPSWRYFSLSTLVFSCNVWPPFPARP
                     PAFLVHFLENVVESEVSG*LGGCFEPGKDKKEDGKD*STFPPGSLAEPPRATWTPWKDL
                     AQAGPPLERQELWI*SRILC*AWCLMPIIPTLWEASVGGSLEPRSSRLAWATQRDPISK
                     IIIIIK*KISRAWWHVPVVPATQEAEARGWLEPGMLRLQ*TVITPLHSSLGKRARDPVS
                     NNNNNNNLILENKETSGFEVPFG*KEKTFTPRNSLCCPEGAEGCIAHVCCAFVLSNSCL
                     LHYLPAGYRVNS*PHLGQIGSKFLFLLRVKRPVFVCCKQPWNSNSSHLFGIWPPSSRMI
                     HGSVQKFIRLSDLRAVGEGFSSRTDGEGSCSPSSTFFASIIHKNTFGRAKNEHCRSLQP
                     CFVTQMHSLL*RPSLKWHLGVHARSWCFMRD*QLSGVVALSEDSPPPVCADDTYTPDNS
                     LSLLCSFYSVANIHMI*NPF*ISIMVHPCPNAESELFVLHYYFQGE*LAFFLQK*LKFL
                     YVAV"
     misc_feature    3135..3140
                     /label=HindIII(1)
                     /note="HindIII(1)"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=HindIII
                     ApEinfo_fwdcolor=#ff0000"
                     /note="color: #ff0000"
     misc_feature    3957..4181
                     /label=BGH polyA signal
                     /note="BGH polyA signal"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff80c0"
                     /note="color: #ff80c0"
     misc_feature    4226..4655
                     /label=F1 origin
                     /note="F1 origin"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#80ff80"
                     /note="color: #80ff80"
     misc_feature    4275..5069
                     /label=Neo/Kan
                     /note="Neo/Kan"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ffff00"
                     /note="color: #ffff00"
     misc_feature    5800..6416
                     /label=Ori
                     /note="Ori"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#80ffff"
                     /note="color: #80ffff"
     misc_feature    6571..7431
                     /label=Amp
                     /note="Amp"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ce5831"
                     /note="color: #ce5831"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcc
      961 accacactgg actagtggat ccgagctcgg taccaagctt cccgggatac ttaggatcca
     1021 gggcgcgcag gcggcgcggg tgcgcggtgc ggcgctggta tccagaggac gcggtcaccg
     1081 cctctggcat ttgtcgttct gcgcttctcc gcaaggaccc tctgttaggc aggcgcccac
     1141 cgtaagcctc ccgggccttg tgaacctgca aacccaagtc tgagagacga tccgccttca
     1201 gcgctttcca gcttggcaga gaggctttcc cggcggggat ctttggttgg cgctggcgat
     1261 gcgcggggaa gaaaggcgag gagcggcgtc caggctgggt gatgtcccag cacgagtagg
     1321 cgggatgcgc tcgcttggtc ctccgggcgc ccggtccctg cccgcgtcgc gcgcccaccc
     1381 ctggggacga gaaggcggcc gcctgaggac ccccgcccgc gacctccgcg agtctggagc
     1441 gcagaggaca gggtctggct gctctttggc cttggatgga aagtggggaa ttgggtgggg
     1501 ggctgcggac cccttaacgt ggattacttg gtgtgtatca gctgggctca gaagacccac
     1561 gacctcttct ccatccgtgg attgatttgt tctgcttaac agctgggtcg ccaagctgga
     1621 ggtatttttc cctctccacc ctggtcttct cctgtaacgt gtggccgcct tttccagcac
     1681 ggcctcctgc cttcctggtg cactttttgg agaacgtggt ggaatcagag gtttctggct
     1741 gactcggtgg gtgctttgaa ccaggaaagg acaagaaaga ggatgggaag gactgatcca
     1801 cattcccacc aggaagttta gcagaacccc cgcgtgccac ctggacccct tggaaggacc
     1861 tggctcaggc tggaccacct cttgagaggc aggagctctg gatttgatca agaattcttt
     1921 gctgagcatg gtgcctcatg cctataatcc caacactttg ggaggccagt gtgggaggat
     1981 ctcttgagcc caggagttca agactagcct gggcaacaca gagagacccc atctctaaaa
     2041 taataataat aataaaataa aaaattagca gggcatggtg gcatgtgcct gtagtcccag
     2101 ctacccagga ggctgaggca agaggatggc tggagcctgg gatgttgagg ctgcaatgaa
     2161 ctgtgattac cccactgcac tccagcctgg gcaaaagagc gagagaccct gtctcaaata
     2221 ataataataa taataatctt attttggaga ataaagagac ctctggattt gaggtgccat
     2281 ttgggtagaa agaaaagacg tttacaccga gaaatagtct gtgttgccct gaaggagcag
     2341 agggatgcat cgctcatgtc tgctgtgcct ttgtactcag caattcttgt ttgctccatt
     2401 atcttccagc cggatacaga gtgaatagtt aaccacactt aggtcaaata ggatctaaat
     2461 ttttgttcct gctccgtgta aagaggccag tgtttgtgtg ttgcaagcag ccttggaata
     2521 gtaactcttc tcatttgttt gggatctggc caccaagttc cagaatgata cacggatcag
     2581 tgcagaagtt catcaggctc tcggacctta gggctgttgg agaaggcttc agcagcagaa
     2641 ctgatggtga aggctcgtgt tctccatcct caactttctt tgcttcgatc atacacaaga
     2701 atacatttgg aagggcaaaa aatgaacact gtcgttcatt gcagccgtgt tttgtgacac
     2761 agatgcacag tctgctgtga agaccttctc tcaagtggca tttgggagtc catgccagat
     2821 catggtgctt catgagagac tgacagctat caggggttgt ggcacttagt gaggactctc
     2881 ctcccccagt gtgtgctgat gacacataca cacctgacaa tagcttgagt cttctctgtt
     2941 ccttttactc tgtagccaac atacacatga tttaaaaccc tttctaaata tctatcatgg
     3001 ttcatccttg tccaaatgca gagtcagagc tatttgtact tcattattat ttccaaggcg
     3061 aatagttggc tttctttttg caaaaataat taaagttttt gtatgttgca gttgctctag
     3121 ataagtatca cgtgaagctt aagtttaaac cgctgatcag cctcgactgt gccttctagt
     3181 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3241 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3301 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3361 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3421 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3481 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3541 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3601 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3661 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3721 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3781 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3841 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3901 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3961 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4021 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4081 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4141 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4201 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4261 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4321 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4381 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4441 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4501 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4561 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4621 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4681 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4741 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4801 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4861 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4921 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4981 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5041 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5101 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5161 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5221 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5281 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5341 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     5401 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5461 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5521 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5581 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5641 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5701 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     6361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7501 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7561 tgacgtc
//
