LOCUS       Exported                6580 bp ds-DNA     circular SYN 20-MAR-2017
DEFINITION  pcDNA3.1(-).
ACCESSION   .
VERSION     .
KEYWORDS    pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 20, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..920,2148..6580)
                     /organism="pcDNA3.1(-)"
                     /mol_type="other DNA"
                     /label=source:pcDNA3.1(-)
                     /note="source:pcDNA3.1(-)"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#fff4fb"
                     /note="This feature has 2 segments:
                        1:    1 ..  920 / #fff4fb
                        2: 2148 .. 6580 / #fff4fb"
     promoter        236..852
                     /label=CMV_immearly_promoter
                     /note="CMV_immearly_promoter ApEinfo_revcolor=pink"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#2de4ff"
                     /note="color: #2de4ff"
     misc_feature    315..602
                     /label=CAG_enhancer
                     /note="CAG_enhancer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    769..789
                     /label=CMV_fwd_primer
                     /note="CMV_fwd_primer"
                     /note="ApEinfo_revcolor=#ff3d58
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff3d58"
                     /note="color: #ff3d58"
     promoter        863..881
                     /label=T7_promoter
                     /note="T7_promoter ApEinfo_revcolor=pink"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    915..920
                     /label=Xba1
                     /note="Xba1"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    921..953
                     /label=MS2 binding protein
                     /note="MS2 binding protein"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    924..947
                     /label=FLAG tag
                     /note="FLAG tag"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#004080"
                     /note="color: #004080"
     misc_feature    954..974
                     /label=SV40 NLS
                     /note="SV40 NLS"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff00ff"
                     /note="color: #ff00ff"
     misc_feature    975..980
                     /label=MS2 binding protein(1)
                     /note="MS2 binding protein(1)"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=MS2 binding protein
                     ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    975..980
                     /label=BamHI
                     /note="BamHI"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff0000"
                     /note="color: #ff0000"
     misc_feature    join(981..1221,1223..1226,1228,1230..1231,1233..1241,
                     1243..1339,1341..1379)
                     /label=MS2 binding protein(2)
                     /note="MS2 binding protein(2)"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=MS2 binding protein
                     ApEinfo_fwdcolor=cyan"
                     /note="This feature has 7 segments:
                        1:  981 .. 1221 / #00ffff
                        2: 1223 .. 1226 / #00ffff
                        3: 1228 .. 1228 / #00ffff
                        4: 1230 .. 1231 / #00ffff
                        5: 1233 .. 1241 / #00ffff
                        6: 1243 .. 1339 / #00ffff
                        7: 1341 .. 1379 / #00ffff"
     misc_feature    1203..1205
                     /label=V75E mutation
                     /note="V75E mutation"
                     /note="ApEinfo_revcolor=#00ff00
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#12ff12"
                     /note="color: #12ff12"
     misc_feature    1221..1223
                     /label=A81G mutation
                     /note="A81G mutation"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#00ff08"
                     /note="color: #00ff08"
     misc_feature    1380..1387
                     /label=NotI
                     /note="NotI"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    1389..1412
                     /label=3C protease site
                     /note="3C protease site"
                     /note="ApEinfo_revcolor=#feff8b
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#feff8b"
                     /note="color: #feff8b"
     misc_feature    1413..1418
                     /label=BamHI(1)
                     /note="BamHI(1)"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=BamHI
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    join(1419..2036,2040..2135)
                     /note="eGFP"
                     /note="ApEinfo_revcolor=#12ff29
                     vntifkey=21
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#12ff29"
                     /note="This feature has 2 segments:
                        1: 1419 .. 2036 / #a6acb3
                        2: 2040 .. 2135 / #a6acb3"
     misc_feature    2148..2153
                     /label=HindIII
                     /note="HindIII"
                     /note="ApEinfo_revcolor=green
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=cyan"
                     /note="color: #00ffff"
     misc_feature    complement(2174..2191)
                     /label=BGH_rev_primer
                     /note="BGH_rev_primer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     terminator      2177..2404
                     /label=bGH_PA_terminator
                     /note="bGH_PA_terminator ApEinfo_revcolor=#29ff6b"
                     /note="ApEinfo_revcolor=#29ff6b
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#29ff6b"
                     /note="color: #29ff6b"
     rep_origin      2467..2773
                     /label=f1_origin
                     /note="f1_origin"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#8efff9"
                     /note="color: #8efff9"
     misc_feature    complement(2887..2907)
                     /label=pBABE_3_primer
                     /note="pBABE_3_primer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(2893..3108)
                     /label=SV40_enhancer
                     /note="SV40_enhancer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        2905..3173
                     /label=SV40_promoter
                     /note="SV40_promoter ApEinfo_revcolor=pink"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff17e1"
                     /note="color: #ff17e1"
     rep_origin      3072..3149
                     /label=SV40_origin
                     /note="SV40_origin"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#3679ff"
                     /note="color: #3679ff"
     misc_feature    3134..3153
                     /label=SV40pro_F_primer
                     /note="SV40pro_F_primer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb"
     CDS             3288..4082
                     /codon_start=1
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="NeoR/KanR"
                     /note="ORF frame 2
                     ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#f3ff50"
                     /note="color: #f3ff50"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3291..4079
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR(1)
                     /note="NeoR/KanR"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=NeoR/KanR
                     ApEinfo_fwdcolor=#faff45"
                     /note="color: #faff45; direction: RIGHT"
     terminator      4259..4378
                     /label=SV40_PA_terminator
                     /note="SV40_PA_terminator ApEinfo_revcolor=pink"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    4347..4366
                     /label=EBV_rev_primer
                     /note="EBV_rev_primer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb"
     misc_feature    complement(4422..4440)
                     /label=M13_reverse_primer
                     /note="M13_reverse_primer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     misc_feature    complement(4439..4461)
                     /label=M13_pUC_rev_primer
                     /note="M13_pUC_rev_primer"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     promoter        complement(4475..4504)
                     /label=lac_promoter
                     /note="lac_promoter ApEinfo_revcolor=pink"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
     rep_origin      complement(4813..5429)
                     /direction=LEFT
                     /label=pBR322_origin
                     /note="pBR322_origin"
                     /note="ApEinfo_revcolor=#6bc4ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#6bc4ff"
                     /note="color: #6bc4ff"
     CDS             complement(5584..6444)
                     /codon_start=1
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="Ampicillin"
                     /note="ORF frame 3
                     ApEinfo_revcolor=#ff8784
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=#ff8784"
                     /note="color: #ff8784"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5584..6444)
                     /gene="Ampicillin"
                     /label=Ampicillin(1)
                     /note="Ampicillin"
                     /note="ApEinfo_revcolor=#ffc28d
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=Ampicillin
                     ApEinfo_fwdcolor=#ffc28d"
                     /note="color: #ffc28d; direction: LEFT"
     promoter        complement(6486..6514)
                     /label=AmpR_promoter
                     /note="AmpR_promoter ApEinfo_revcolor=pink"
                     /note="ApEinfo_revcolor=pink
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_fwdcolor=pink"
                     /note="color: #ffc0cb; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga atggactaca aagacgatga cgacaaggga tccccgaaga
      961 agaaacgcaa agtgggatcc gcttctaact ttactcagtt cgttctcgtc gacaatggcg
     1021 gaactggcga cgtgactgtc gccccaagca acttcgctaa cggggtcgct gaatggatca
     1081 gctctaactc gcgatcacag gcttacaaag taacctgtag cgttcgtcag agctctgcgc
     1141 agaatcgcaa atacaccatc aaagtcgagg tgcctaaagt ggcaacccag actgttggtg
     1201 gtgaagagct tcctgtagcc ggatggagat cttacttaaa tatggaacta accattccaa
     1261 ttttcgccac gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag
     1321 atggaaaccc gattccctcg gcaatcgcag caaactccgg catctacgta atcgccaccg
     1381 cggccgctct cgaggtgctg ttccagggac ccggatccat ggtgagcaag ggcgaggagc
     1441 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1501 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1561 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1621 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1681 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1741 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1801 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1861 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1921 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1981 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2041 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2101 ccgggatcac tctcggcatg gacgagctgt acaagggagg gggctgaaag cttaagttta
     2161 aaccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2221 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2281 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     2341 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     2401 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     2461 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2521 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2581 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2641 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2701 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2761 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2821 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     2881 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2941 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3001 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3061 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3121 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     3181 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     3241 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3301 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3361 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3421 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     3481 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3541 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3601 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3661 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3721 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3781 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     3841 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3901 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     3961 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4021 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4081 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4141 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4201 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     4261 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4321 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4381 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     4441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5341 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5401 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5461 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5521 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5581 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5641 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5701 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5761 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5821 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5881 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5941 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6001 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6061 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6121 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6181 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6241 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6301 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6361 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6421 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6481 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6541 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
