LOCUS       Exported                3534 bp ds-DNA     circular SYN 14-MAR-2017
DEFINITION  Synthetic plasmid pK18 (pBRNeo/pUC18) kanamycin resistance gene, 
            complete cds.
ACCESSION   .
VERSION     .
KEYWORDS    93 pK19pA-MN (published seq) copy
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Pridmore, R.D.
  TITLE     New and versatile cloning vectors with kanamycin-resistance marker.
  JOURNAL   Gene,  56,  2-3,  309-312,  (1987)
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 14, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
COMMENT     Database Reference:
              GENBANK    SYN8KMRCG
              Accession:  M17626
            ------------
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        227..276
                     /label=NEOKAN_promoter
                     /note="NEOKAN_promoter"
     CDS             365..1159
                     /codon_start=1
                     /note="kanamycin"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            368..1156
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="NeoR/KanR"
     rep_origin      1427..2046
                     /label=pBR322_origin
                     /note="pBR322_origin"
     CDS             2307..2428
                     /codon_start=1
                     /note="REP E. coli lacI repressor gene"
                     /translation="AQRN*CELAHSLGTPGFTLYASGSYVVWNCERITISHRKQ"
     promoter        2355..2384
                     /label=lac_promoter
                     /note="lac_promoter"
     primer_bind     2398..2420
                     /label=M13_pUC_rev_primer
                     /note="M13_pUC_rev_primer"
     primer_bind     2419..2437
                     /label=M13_reverse_primer
                     /note="M13_reverse_primer promoter"
     CDS             2429..3355
                     /codon_start=1
                     /note="proteinA-MNase A"
                     /translation="MTMITPSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAF
                     YEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFY
                     EILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKDDDKEFATSTKKLHK
                     EPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVEN
                     AKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRK
                     SEAQAKKEKLNIWSEDNADSGQ**GSPG"
     CDS             2429..3337
                     /codon_start=1
                     /note="pA-MN"
                     /translation="MTMITPSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAF
                     YEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFY
                     EILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKDDDKEFATSTKKLHK
                     EPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVEN
                     AKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRK
                     SEAQAKKEKLNIWSEDNADSGQ"
     misc_feature    2872..2890
                     /note="Linker"
     CDS             2891..3337
                     /codon_start=1
                     /note="MN"
                     /note="Not Mammalian optimized"
                     /translation="ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETK
                     HPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALV
                     RQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ"
     primer_bind     complement(3376..3392)
                     /label=M13_forward20_primer
                     /note="M13_forward20_primer promoter"
     misc_feature    3385..3528
                     /label=lacZ_a
                     /note="lacZ_a"
     primer_bind     complement(3385..3407)
                     /label=M13_pUC_fwd_primer
                     /note="M13_pUC_fwd_primer"
ORIGIN
        1 cgataagcta gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
       61 gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
      121 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
      181 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
      241 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
      301 gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt
      361 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
      421 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
      481 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
      541 actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
      601 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
      661 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
      721 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
      781 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
      841 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc
      901 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
      961 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     1021 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     1081 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     1141 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     1201 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     1261 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     1321 ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1381 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1441 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1501 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1561 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1621 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1681 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1741 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1801 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     1861 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1921 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1981 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2041 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2101 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2161 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2221 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2281 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     2341 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     2401 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct taaaagatga
     2461 cccaagccaa agtgctaacc tattgtcaga agctaaaaag ttaaatgaat ctcaagcacc
     2521 gaaagcggat aacaaattca acaaagaaca acaaaatgct ttctatgaaa tcttacattt
     2581 acctaactta aacgaagaac aacgcaatgg tttcatccaa agcctaaaag atgacccaag
     2641 ccaaagcgct aaccttttag cagaagctaa aaagctaaat gatgctcaag caccaaaagc
     2701 tgacaacaaa ttcaacaaag aacaacaaaa tgctttctat gaaattttac atttacctaa
     2761 cttaactgaa gaacaacgta acggcttcat ccaaagcctt aaagacgatc cttcagtgag
     2821 caaagaaatt ttagcagaag ctaaaaagct aaacgatgct caagcaccaa aagatgacga
     2881 taaagaattc gcaacttcaa ctaaaaaatt acataaagaa cctgcgactt taattaaagc
     2941 gattgatggt gatacggtta aattaatgta caaaggtcaa ccaatgacat tcagactatt
     3001 attggtcgac acacctgaaa caaagcatcc taaaaaaggt gtagagaaat atggtcctga
     3061 agcaagtgca tttaccaaaa aaatggtaga aaatgcaaag aaaattgaag tcgaattcga
     3121 caaaggtcaa agaactgata aatatggacg tgggctagcg tatatttatg ctgatggaaa
     3181 aatggtaaac gaagctttag ttcgtcaagg cttggctaaa gttgcttatg tttacaaacc
     3241 taacaataca catgaacaac atttaagaaa aagtgaagca caagcgaaaa aagagaaatt
     3301 aaatatttgg agcgaagaca acgctgattc aggtcaataa tgaggatccc ccgggtaccg
     3361 agctcgaatt aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3421 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3481 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcg
//
