LOCUS       pCAG-Trex2-bpA      5557 bp    DNA     circular UNA 12-DEC-2009
DEFINITION  
ACCESSION   pCAG-cre-bpA
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     promoter        15..1685
                     /note="Oriinal location: 87..1753"
                     /label="CAG Promoter"
     promoter        1695..1717
                     /label="T7 promoter"
     CDS             1727..2437
                     /label="Trex2 coding"
     polyA_signal    2472..2743
                     /note="Oriinal location: 2900..3171"
                     /note="pA from bovine growth hormone"
                     /label=bpA
     misc_feature    complement(2787..2803)
                     /label="M13 for"
     CDS             3531..4291
                     /label="amp resistance"
     misc_feature    5541..5556
                     /label="M13 rev"
ORIGIN
        1 GGCGCGCCGG ATTCGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC
       61 ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC
      121 TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT
      181 AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA
      241 CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG
      301 TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA
      361 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTCGAG GTGAGCCCCA CGTTCTGCTT
      421 CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT
      481 ATTTTGTGCA GCGATGGGGG CGGGGGGGGG GGGGggGCGC GCGCCAGGCG GGGCGGGGCG
      541 GGGCGAGGGG CGGGGCGGGG CGAGGCGGAG AGGTGCGGCG GCAGCCAATC AGAGCGGCGC
      601 GCTCCGAAAG TTTCCTTTTA TGGCGAGGCG GCGGCGGCGG CGGCCCTATA AAAAGCGAAG
      661 CGCGCGGCGG GCGGGAGTCG CTGCGTcgcG CCTTCGCCCC GTGCCCCGCT CCGCcGCCGC
      721 CTCGCGCCGC CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA GCGGGCGGGA
      781 CGGCCCTTCT CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTCG TTTCTTTTCT
      841 GTGGCTGCGT GAAAGCCTTA AAGGGCTCCG GGAGGGCCCT TTGTGCGGGG GGGAGCGGCT
      901 CGGGGGGTGC GTGCGTGTGT GTGTGCGTGG GGAGCGCCGC GTGCGGCCCG CGCTGCCCGG
      961 CGGCTGTGAG CGCTGCGGGC GCGGCGCGGG GCTTTGTGCG CTCCGCGTGT GCGCGAGGGG
     1021 AGCGCGGCCG GGGGCGGTGC CCCGCGGTGC GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG
     1081 TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGGCGGTC GGGCTGTAAC
     1141 CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC
     1201 CGTGCGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG
     1261 CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAGGGGCG CGGCGGCCCC
     1321 GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA TGGTAATCGT
     1381 GCGAGAGGGC GCAGGGACTT CCTTTGTCCC AAATCTGGCG GAGCCGAAAT CTGGGAGGCG
     1441 CCGCCGCACC CCCTCTAGCG GGCGCGGGCG AAGCGGTGCG GCGCCGGCAG GAAGGAAATG
     1501 GGCGGGGAGG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCATCT CCAGCCTCGG
     1561 GGCTGCCGCA GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT
     1621 GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC
     1681 TACAGATCCT TAATTAATAA TACGACTCAC TATAGGGgcc gccaccATGT CTGAGCCACC
     1741 CCGGGCTGAG ACCTTTGTAT TCCTGGACCT AGAAGCCACT GGGCTCCCAA ACATGGATCC
     1801 TGAGATTGCA GAGATATCCC TTTTTGCTGT TCACCGCTCT TCCCTGGAGA ACCCAGAACG
     1861 GGATGATTCT GGTTCCTTGG TGCTGCCCCG TGTTCTGGAC AAGCTCACAC TGTGCATGTG
     1921 CCCGGAGCGC CCCTTTACTG CCAAGGCCAG TGAGATTACT GGTTTGAGCA GCGAAAGCCT
     1981 GATGCACTGC GGGAAGGCTG GTTTCAATGG CGCTGTGGTA AGGACACTGC AGGGCTTCCT
     2041 AAGCCGCCAG GAGGGCCCCA TCTGCCTTGT GGCCCACAAT GGCTTCGATT ATGACTTCCC
     2101 ACTGCTGTGC ACGGAGCTAC AACGTCTGGG TGCCCATCTG CCCCAAGACA CTGTCTGCCT
     2161 GGACACACTG CCTGCATTGC GGGGCCTGGA CCGTGCTCAC AGCCACGGCA CCAGGGCTCA
     2221 AGGCCGCAAA AGCTACAGCC TGGCCAGTCT CTTCCACCGC TACTTCCAGG CTGAACCCAG
     2281 TGCTGCCCAT TCAGCAGAAG GTGATGTGCA CACCCTGCTT CTGATCTTCC TGCATCGTGC
     2341 TCCTGAGCTG CTCGCCTGGG CAGATGAGCA GGCCCGCAGC TGGGCTCATA TTGAGCCCAT
     2401 GTACGTGCCA CCTGATGGTC CAAGCCTCGA AGCCTGAACG CGTAAATGAT TGCAGATCCA
     2461 CTAGTTCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG
     2521 TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT
     2581 AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG
     2641 GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG
     2701 CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCGAGA TCCACTAGTT
     2761 CTAGCCTCGA GGCTAGAGCG GCCGCCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA
     2821 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT
     2881 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA
     2941 TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG
     3001 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC
     3061 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA
     3121 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT
     3181 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT
     3241 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT
     3301 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA
     3361 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA
     3421 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
     3481 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
     3541 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
     3601 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
     3661 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT
     3721 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG
     3781 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT
     3841 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG
     3901 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA
     3961 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG
     4021 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA
     4081 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC
     4141 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC
     4201 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA
     4261 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA
     4321 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA
     4381 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC
     4441 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
     4501 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
     4561 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
     4621 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
     4681 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     4741 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     4801 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     4861 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     4921 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG
     4981 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
     5041 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
     5101 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
     5161 ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG
     5221 CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC
     5281 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA
     5341 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
     5401 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
     5461 AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG
     5521 GATAACAATT TCACACAGGA AACAGCTATG ACCATGA
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