LOCUS       U6-(BbsI)sgRNA_CAG-Venus-bpA      5968 bp    DNA     circular UNA 12-DEC-2009
DEFINITION  
ACCESSION   pCAG-cre-bpA
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     misc_feature    3..18
                     /label="M13 rev"
                     /label=""M13 rev""
     promoter        29..288
                     /label="U6-chimaeric RNA"
                     /label="U6 promoter"
     CDS             285..306
                     /label="target oligo A"
                     /label=""target oligo A""
     misc_feature    285..288
                     /label="BbsI overhang"
     CDS             complement(289..310)
                     /label="target oligo B"
                     /label=""target oligo B""
     misc_feature    289
                     /label=+1
                     /label="+1 transcription start"
     CDS             290..306
                     /label="replace by 20 nt target"
                     /label=""replace by 20 nt target""
     misc_feature    complement(291..296)
                     /label=BbsI
                     /label="BbsI binding site"
     misc_feature    299..304
                     /label=BbsI
                     /label="BbsI binding site"
     misc_feature    307..389
                     /label="sgRNA scaffold"
     misc_feature    complement(307..310)
                     /label="BbsI overhang"
     promoter        417..2087
                     /note="Oriinal location: 87..1753"
                     /label="CAG Promoter"
                     /label=""CAG Promoter""
     misc_feature    2086..2087
                     /note="Oriinal location: 1752..1753"
                     /label="splice acceptor"
                     /label=""splice acceptor""
     promoter        2097..2119
                     /label=T7
     CDS             2129..2869
                     /label=venus
                     /label=NLS-venus
     CDS             2870..2902
                     /label="7xG-TRN tag"
     polyA_signal    2925..3195
                     /note="Oriinal location: 2900..3171"
                     /note="pA from bovine growth hormone"
                     /label=bpA
     misc_feature    complement(3217..3233)
                     /label="M13 for"
                     /label=""M13 for""
     CDS             3961..4821
                     /label="amp resistance"
ORIGIN
        1 GAAACAGCTA TGACCATGAG GCGCGCCCCC CCCTTCACCG AGGGCCTATT TCCCATGATT
       61 CCTTCATATT TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT
      121 GTAAACACAA AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG
      181 TTTGCAGTTT TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG
      241 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACCGG GTCTTCGAGA
      301 AGACCTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA
      361 AAAAGTGGCA CCGAGTCGGT GCTTTTTTTG ATCCAAGGGT GGGGCGCGCC GGATTCGACA
      421 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA
      481 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA
      541 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT
      601 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT
      661 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA
      721 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
      781 CATCGCTATT ACCATGGTCG AGGTGAGCCC CACGTTCTGC TTCACTCTCC CCATCTCCCC
      841 CCCCTCCCCA CCCCCAATTT TGTATTTATT TATTTTTTAA TTATTTTGTG CAGCGATGGG
      901 GGCGGGGGGG GGGGGGGGGC GCGCGCCAGG CGGGGCGGGG CGGGGCGAGG GGCGGGGCGG
      961 GGCGAGGCGG AGAGGTGCGG CGGCAGCCAA TCAGAGCGGC GCGCTCCGAA AGTTTCCTTT
     1021 TATGGCGAGG CGGCGGCGGC GGCGGCCCTA TAAAAAGCGA AGCGCGCGGC GGGCGGGAGT
     1081 CGCTGCGTCG CGCCTTCGCC CCGTGCCCCG CTCCGCCGCC GCCTCGCGCC GCCCGCCCCG
     1141 GCTCTGACTG ACCGCGTTAC TCCCACAGGT GAGCGGGCGG GACGGCCCTT CTCCTCCGGG
     1201 CTGTAATTAG CGCTTGGTTT AATGACGGCT CGTTTCTTTT CTGTGGCTGC GTGAAAGCCT
     1261 TAAAGGGCTC CGGGAGGGCC CTTTGTGCGG GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT
     1321 GTGTGTGCGT GGGGAGCGCC GCGTGCGGCC CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG
     1381 GCGCGGCGCG GGGCTTTGTG CGCTCCGCGT GTGCGCGAGG GGAGCGCGGC CGGGGGCGGT
     1441 GCCCCGCGGT GCGGGGGGGC TGCGAGGGGA ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG
     1501 GGGGGTGAGC AGGGGGTGTG GGCGCGGCGG TCGGGCTGTA ACCCCCCCCT GCACCCCCCT
     1561 CCCCGAGTTG CTGAGCACGG CCCGGCTTCG GGTGCGGGGC TCCGTGCGGG GCGTGGCGCG
     1621 GGGCTCGCCG TGCCGGGCGG GGGGTGGCGG CAGGTGGGGG TGCCGGGCGG GGCGGGGCCG
     1681 CCTCGGGCCG GGGAGGGCTC GGGGGAGGGG CGCGGCGGCC CCGGAGCGCC GGCGGCTGTC
     1741 GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG GCGCAGGGAC
     1801 TTCCTTTGTC CCAAATCTGG CGGAGCCGAA ATCTGGGAGG CGCCGCCGCA CCCCCTCTAG
     1861 CGGGCGCGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT
     1921 GCGTCGCCGC GCCGCCGTCC CCTTCTCCAT CTCCAGCCTC GGGGCTGCCG CAGGGGGACG
     1981 GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT
     2041 CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGATC CTTAATTAAT
     2101 AATACGACTC ACTATAGGGG CCGCCACCAT GCCCAAGAAG AAGAGGAAGG TGATGGTGAG
     2161 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
     2221 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
     2281 GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
     2341 CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA
     2401 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
     2461 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
     2521 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
     2581 GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA
     2641 GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA
     2701 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
     2761 CTACCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA
     2821 GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGG GCGGAGGCGG
     2881 AGGCGGAGGC ACGCGTAATT GATTGCAGAT CCACTAGTTC TAGAGCTCGC TGATCAGCCT
     2941 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA
     3001 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT
     3061 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG
     3121 ATTGGGAAGA GAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG
     3181 AAAGAACCAG CTGGGCTCGA GGCTAGAGCG GCCGCCACTG GCCGTCGTTT TACAACGTCG
     3241 TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC
     3301 CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT
     3361 GAATGGCGAA TGGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC
     3421 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC
     3481 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT
     3541 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG
     3601 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC
     3661 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA
     3721 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT
     3781 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT
     3841 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG
     3901 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT
     3961 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT
     4021 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA
     4081 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC
     4141 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC
     4201 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG
     4261 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA
     4321 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC
     4381 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT
     4441 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG
     4501 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT
     4561 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC
     4621 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT
     4681 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC
     4741 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC
     4801 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT
     4861 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG
     4921 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
     4981 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA
     5041 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG
     5101 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA
     5161 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA
     5221 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     5281 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     5341 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG
     5401 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     5461 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     5521 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     5581 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG
     5641 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT
     5701 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA
     5761 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG
     5821 CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG
     5881 CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA
     5941 ATTGTGAGCG GATAACAATT TCACACAG
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