LOCUS       pFA6a_CLIP_kanMX        4686 bp ds-DNA     linear       02-JUN-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     gene            85..589
                     /label=CLIP
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1..1
                     /label=SP6
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(590..628)
                     /label=JB'12
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      629..816
                     /label=ADH1 terminator
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3359..4219
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        863..1205
                     /label=pTEF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1207..2016
                     /label=kanMX6
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2017..2249
                     /label=TEFterm
                     /ApEinfo_fwdcolor=#004080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(609..628)
                     /label=JB' 1
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     43..62
                     /label=JB' 89
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     43..84
                     /label=JB' 94
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     63..84
                     /label=JB' 11
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1312..1331
                     /label=Target_KanMX6_CRISPR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2323..2342)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(2582..3264)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3362..4021)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     590..605
                     /label=JB'12(1)
                     /ApEinfo_label=JB'12
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     63..84
                     /label=JB'94
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1047..1066
                     /label=JB'150
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1377..1397
                     /label=JB'152
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1291..1311
                     /label=JB'151
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1589..1608
                     /label=JB'153
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1569..1585
                     /label=JB'154
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1312..1331
                     /label=Target_area_KanMX6_CRISPR
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTCGTACG CTGCAGGTCG ACggatcccc gggttaatta
       61 acGACAAAGA CTGCGAAATG AAGCGCACCA CCCTGGATAG CCCTCTGGGC AAGCTGGAAC
      121 TGTCTGGGTG CGAACAGGGC CTGCACCGTA TCATCTTCCT GGGCAAAGGA ACATCTGCCG
      181 CCGACGCCGT GGAAGTGCCT GCCCCAGCCG CCGTGCTGGG CGGACCAGAG CCACTGATCC
      241 AGGCCACCGC CTGGCTCAAC GCCTACTTTC ACCAGCCTGA GGCCATCGAG GAGTTCCCTG
      301 TGCCAGCCCT GCACCACCCA GTGTTCCAGC AGGAGAGCTT TACCCGCCAG GTGCTGTGGA
      361 AACTGCTGAA AGTGGTGAAG TTCGGAGAGG TCATCAGCGA GAGCCACCTG GCCGCCCTGG
      421 TGGGCAATCC CGCCGCCACC GCCGCCGTGA ACACCGCCCT GGACGGAAAT CCCGTGCCCA
      481 TTCTGATCCC CTGCCACCGG GTGGTGCAGG GCGACAGCGA CGTGGGGCCC TACCTGGGCG
      541 GGCTCGCCGT GAAAGAGTGG CTGCTGGCCC ACGAGGGCCA CAGACTGGGC AAGCCTGGGC
      601 TGGGTTAGgg cgcgccactt ctaaataaGC GAATTTCTTA TGATTTATGA TTTTTATTAT
      661 TAAATAAGTT ATAAAAAAAA TAAGTGTATA CAAATTTTAA AGTGACTCTT AGGTTTTAAA
      721 ACGAAAATTC TTATTCTTGA GTAACTCTTT CCTGTAGGTC AGGTTGCTTT CTCAGGTATA
      781 GCATGAGGTC GCTCTTATTG ACCACACCTC TACCGGCAGA TCTGTTTAGC TTGCCTCGTC
      841 CCCGCCGGGT CACCCGGCCA GCGACATGGA GGCCCAGAAT ACCCTCCTTG ACAGTCTTGA
      901 CGTGCGCAGC TCAGGGGCAT GATGTGACTG TCGCCCGTAC ATTTAGCCCA TACATCCCCA
      961 TGTATAATCA TTTGCATCCA TACATTTTGA TGGCCGCACG GCGCGAAGCA AAAATTACGG
     1021 CTCCTCGCTG CAGACCTGCG AGCAGGGAAA CGCTCCCCTC ACAGACGCGT TGAATTGTCC
     1081 CCACGCCGCG CCCCTGTAGA GAAATATAAA AGGTTAGGAT TTGCCACTGA GGTTCTTCTT
     1141 TCATATACTT CCTTTTAAAA TCTTGCTAGG ATACAGTTCT CACATCACAT CCGAACATAA
     1201 ACAACCATGG GTAAGGAAAA GACTCACGTT TCGAGGCCGC GATTAAATTC CAACATGGAT
     1261 GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC
     1321 TATCGATTGT ATGGGAAGCC CGATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC
     1381 GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT
     1441 CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG
     1501 ATCCCCGGCA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT
     1561 GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT
     1621 TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG
     1681 GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA
     1741 GAAATGCATA AGCTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA
     1801 CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC
     1861 GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT
     1921 CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA
     1981 TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG TACTGACAAT AAAAAGATTC
     2041 TTGTTTTCAA GAACTTGTCA TTTGTATAGT TTTTTTATAT TGTAGTTGTT CTATTTTAAT
     2101 CAAATGTTAG CGTGATTTAT ATTTTTTTTC GCCTCGACAT CATCTGCCCA GATGCGAAGT
     2161 TAAGTGCGCA GAAAGTAATA TCATGCGTCA ATCGTATGTG AATGCTGGTC GCTATACTGC
     2221 TGTCGATTCG ATACTAACGC CGCCATCCAG TTTAAACGAG CTCGAATTCA TCGATGATAT
     2281 CAGATCCACT AGTGGCCTAT GCGGCCGCGG ATCTGCCGGT CTCCCTATAG TGAGTCGTAT
     2341 TAATTTCGAT AAGCCAGGTT AACCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
     2401 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
     2461 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
     2521 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
     2581 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
     2641 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
     2701 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     2761 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT
     2821 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     2881 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     2941 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     3001 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
     3061 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     3121 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     3181 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     3241 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     3301 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     3361 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     3421 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     3481 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     3541 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     3601 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     3661 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     3721 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     3781 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     3841 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     3901 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     3961 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     4021 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     4081 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     4141 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     4201 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     4261 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     4321 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT
     4381 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG
     4441 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG
     4501 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC
     4561 TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGGAC ATATTGTCGT
     4621 TAGAACGCGG CTACAATTAA TACATAACCT TATGTATCAT ACACATACGA TTTAGGTGAC
     4681 ACTATA     
//
