LOCUS       pFA6a_PATagRFP_k        4833 bp ds-DNA     linear       08-MAR-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     4817..4833
                     /label=SP6
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1..1
                     /label=SP6(1)
                     /ApEinfo_label=SP6
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            88..734
                     /label=PATagRFP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2470..2489)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(2729..3411)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3509..4168)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      776..963
                     /label=ADH1 terminator
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3506..4366
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1010..1352
                     /label=pTEF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            66..752
                     /label=PATagRFP(1)
                     /ApEinfo_label=PATagRFP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1354..2163
                     /label=kanMX6
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2164..2396
                     /label=TEFterm
                     /ApEinfo_fwdcolor=#004080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     43..62
                     /label=JB' 89
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     43..87
                     /label=JB' 91
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     63..87
                     /label=JB' 5
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(735..775)
                     /label=JB'8
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     756..775
                     /label=JB' 1
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTCGTACG CTGCAGGTCG ACggatcccc gggttaatta
       61 acAGCGAGCT GATTAAGGAG AACATGCACA TGAAGCTGTA CATGGAGGGC ACCGTGAACA
      121 ACCACCACTT CAAGTGCACA TCCGAGGGCG AAGGCAAGCC CTACGAGGGC ACCCAGACCA
      181 TGAGAATCAA GGTGGTCGAG GGCGGCCCTC TCCCCTTCGC CTTCGACATC CTGGCTACCA
      241 GCTTCATGTA CGGCAGCAGC ACCTTCATCA ACCACACCCA GGGCATCCCC GACTTCTGGA
      301 AGCAGTCCTT CCCTGAGGGC TTCACATGGG AGAGAGTCAC CACATACGAA GACGGGGGCG
      361 TGCTGACCGC TACCCAGGAC ACCAGCCTCC AGGACGGCTG CCTCATCTAC AACGTCAAGA
      421 TCAGAGGGGT GAACTTCCCA TCCAACGGCC CTGTGATGAA GAAGAAAACA CTCGGCTGGG
      481 AACCGAGCAC CGAGAAACTG AAACCCGCTG ACGGCGGCCT GGAAGGCAGG GTCGACATGG
      541 CCCTGAAGCT CGTGGGCGGG GGCCACCTGA TCTGCAACTT CAAGACCACA TACAGATCCA
      601 AGAAACCCGC TAAGAACCTC AAGATGCCCG GCGTCTACTA TGTGGACCGC AGACTGGAAA
      661 TCATCAAGGA GGCCGACAAA GAGACCTACT GGGAGCAGCA CGAGGTGGCT GTGGCCAGAT
      721 ACTCCGACCT CCCTAGCAAA CTGGGGCACA AGTAAggcgc gccacttcta aataaGCGAA
      781 TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA AAAAAAATAA GTGTATACAA
      841 ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA TTCTTGAGTA ACTCTTTCCT
      901 GTAGGTCAGG TTGCTTTCTC AGGTATAGCA TGAGGTCGCT CTTATTGACC ACACCTCTAC
      961 CGGCAGATCT GTTTAGCTTG CCTCGTCCCC GCCGGGTCAC CCGGCCAGCG ACATGGAGGC
     1021 CCAGAATACC CTCCTTGACA GTCTTGACGT GCGCAGCTCA GGGGCATGAT GTGACTGTCG
     1081 CCCGTACATT TAGCCCATAC ATCCCCATGT ATAATCATTT GCATCCATAC ATTTTGATGG
     1141 CCGCACGGCG CGAAGCAAAA ATTACGGCTC CTCGCTGCAG ACCTGCGAGC AGGGAAACGC
     1201 TCCCCTCACA GACGCGTTGA ATTGTCCCCA CGCCGCGCCC CTGTAGAGAA ATATAAAAGG
     1261 TTAGGATTTG CCACTGAGGT TCTTCTTTCA TATACTTCCT TTTAAAATCT TGCTAGGATA
     1321 CAGTTCTCAC ATCACATCCG AACATAAACA ACCATGGGTA AGGAAAAGAC TCACGTTTCG
     1381 AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT
     1441 AATGTCGGGC AATCAGGTGC GACAATCTAT CGATTGTATG GGAAGCCCGA TGCGCCAGAG
     1501 TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA
     1561 CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT
     1621 GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGCAAAA CAGCATTCCA GGTATTAGAA
     1681 GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG
     1741 CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG
     1801 GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT
     1861 GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAGC TTTTGCCATT CTCACCGGAT
     1921 TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA
     1981 ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC
     2041 CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT
     2101 GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC
     2161 TAATCAGTAC TGACAATAAA AAGATTCTTG TTTTCAAGAA CTTGTCATTT GTATAGTTTT
     2221 TTTATATTGT AGTTGTTCTA TTTTAATCAA ATGTTAGCGT GATTTATATT TTTTTTCGCC
     2281 TCGACATCAT CTGCCCAGAT GCGAAGTTAA GTGCGCAGAA AGTAATATCA TGCGTCAATC
     2341 GTATGTGAAT GCTGGTCGCT ATACTGCTGT CGATTCGATA CTAACGCCGC CATCCAGTTT
     2401 AAACGAGCTC GAATTCATCG ATGATATCAG ATCCACTAGT GGCCTATGCG GCCGCGGATC
     2461 TGCCGGTCTC CCTATAGTGA GTCGTATTAA TTTCGATAAG CCAGGTTAAC CTGCATTAAT
     2521 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC
     2581 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
     2641 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG
     2701 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
     2761 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
     2821 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
     2881 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
     2941 AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
     3001 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     3061 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
     3121 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     3181 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
     3241 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     3301 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     3361 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
     3421 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
     3481 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
     3541 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
     3601 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC
     3661 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
     3721 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
     3781 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
     3841 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
     3901 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
     3961 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
     4021 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
     4081 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
     4141 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
     4201 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
     4261 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
     4321 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
     4381 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
     4441 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG
     4501 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
     4561 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA
     4621 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG
     4681 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA
     4741 GAGTGCACCA TATGGACATA TTGTCGTTAG AACGCGGCTA CAATTAATAC ATAACCTTAT
     4801 GTATCATACA CATACGATTT AGGTGACACT ATA  
//
