LOCUS       pFA6a_PAmKate_ka        4851 bp ds-DNA     linear       02-JUN-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     4835..4851
                     /label=SP6
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1..1
                     /label=SP6(1)
                     /ApEinfo_label=SP6
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            82..753
                     /label=PAmKate
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1028..1370
                     /label=pTEF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(754..793)
                     /label=JB'10
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      794..981
                     /label=ADH1 terminator
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3524..4384
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        1028..1370
                     /label=pTEF(1)
                     /ApEinfo_label=pTEF
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1372..2181
                     /label=kanMX6
                     /ApEinfo_fwdcolor=#ff8040
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2182..2414
                     /label=TEFterm
                     /ApEinfo_fwdcolor=#004080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     774..793
                     /label=JB' 1
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     43..62
                     /label=JB' 89
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     43..81
                     /label=JB' 93
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     63..81
                     /label=JB' 9
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(2488..2507)
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(2747..3429)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3527..4186)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1212..1231
                     /label=JB'150
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1542..1562
                     /label=JB'152
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1456..1476
                     /label=JB'151
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1754..1773
                     /label=JB'153
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1734..1750
                     /label=JB'154
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1477..1496
                     /label=Target_area_KanMX6_CRISPR
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     63..81
                     /label=JB'93
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     754..773
                     /label=JB'10(1)
                     /ApEinfo_label=JB'10
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 GAACGCGGCC GCCAGCTGAA GCTTCGTACG CTGCAGGTCG ACggatcccc gggttaatta
       61 acGTGTCTAA GGGCGAAGAG CTGATTAAGG AGAACATGCA CATGAAGCTG TACATGGAGG
      121 GCACCGTGAA CAACCACCAC TTCAAGTGCA CATCCGAGGG CGAAGGCAAG CCCTACGAGG
      181 GCACCCAGAC CATGAGAATC AAGGTGGTCG AGGGCGGCCC TCTCCCCTTC GCCTTCGACA
      241 TCCTGGCTAC CAGCTTCATG TACGGCAGCA AGACCTTCAT CAACCACACC CAGGGCATCC
      301 CCGACTTCTG GAAGCAGTCC TTCCCTGAGG GCTTCACATG GGAGAGAGTC ACCACATACG
      361 AAGACGGGGG CGTGCTGACC GCTACCCAGG ACACCAGCCT CCAGGACGGC TGCCTCATCT
      421 ACAACGTCAA GATCAGAGGG GTGAACTTCC CATCCAACGG CCCTGTGATG CAGAAGAAAA
      481 CACTCGGCTG GGAGGCCAAC ACCGAGATGC TGTACCCCGC TGACGGCGGC CTGGAAGGCA
      541 GAGGGGACAT GGCCCTGAAG CTCGTGGGCG GGGGCCACCT GATCTGCAAC TTGAAGACCA
      601 CATACAGATC CAAGAAACCC GCTAAGAACC TCAAGATGCC CGGCGTCTAC TATGTGGACC
      661 GCAGACTGGA AAGAATCAAG GAGGCCGACA AAGAGACCTA CGTCGAGCAG CACGAGGTGG
      721 CTGTGGCCAG ATACTGCGAC CTCCCTAGCA AACTGGGGCA CAAGCTTAAT TAAggcgcgc
      781 cacttctaaa taaGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA
      841 AAAAATAAGT GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT
      901 CTTGAGTAAC TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGCATG AGGTCGCTCT
      961 TATTGACCAC ACCTCTACCG GCAGATCTGT TTAGCTTGCC TCGTCCCCGC CGGGTCACCC
     1021 GGCCAGCGAC ATGGAGGCCC AGAATACCCT CCTTGACAGT CTTGACGTGC GCAGCTCAGG
     1081 GGCATGATGT GACTGTCGCC CGTACATTTA GCCCATACAT CCCCATGTAT AATCATTTGC
     1141 ATCCATACAT TTTGATGGCC GCACGGCGCG AAGCAAAAAT TACGGCTCCT CGCTGCAGAC
     1201 CTGCGAGCAG GGAAACGCTC CCCTCACAGA CGCGTTGAAT TGTCCCCACG CCGCGCCCCT
     1261 GTAGAGAAAT ATAAAAGGTT AGGATTTGCC ACTGAGGTTC TTCTTTCATA TACTTCCTTT
     1321 TAAAATCTTG CTAGGATACA GTTCTCACAT CACATCCGAA CATAAACAAC CATGGGTAAG
     1381 GAAAAGACTC ACGTTTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG
     1441 TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG ATTGTATGGG
     1501 AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT
     1561 ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG
     1621 CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC CGGCAAAACA
     1681 GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA
     1741 GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC
     1801 GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT
     1861 TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAGCTT
     1921 TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT
     1981 TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA
     2041 TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA
     2101 CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG
     2161 ATGCTCGATG AGTTTTTCTA ATCAGTACTG ACAATAAAAA GATTCTTGTT TTCAAGAACT
     2221 TGTCATTTGT ATAGTTTTTT TATATTGTAG TTGTTCTATT TTAATCAAAT GTTAGCGTGA
     2281 TTTATATTTT TTTTCGCCTC GACATCATCT GCCCAGATGC GAAGTTAAGT GCGCAGAAAG
     2341 TAATATCATG CGTCAATCGT ATGTGAATGC TGGTCGCTAT ACTGCTGTCG ATTCGATACT
     2401 AACGCCGCCA TCCAGTTTAA ACGAGCTCGA ATTCATCGAT GATATCAGAT CCACTAGTGG
     2461 CCTATGCGGC CGCGGATCTG CCGGTCTCCC TATAGTGAGT CGTATTAATT TCGATAAGCC
     2521 AGGTTAACCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
     2581 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
     2641 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
     2701 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
     2761 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
     2821 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
     2881 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
     2941 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
     3001 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
     3061 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
     3121 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
     3181 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
     3241 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
     3301 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
     3361 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
     3421 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
     3481 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
     3541 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
     3601 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
     3661 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
     3721 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
     3781 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
     3841 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
     3901 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
     3961 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
     4021 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
     4081 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
     4141 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
     4201 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
     4261 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
     4321 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
     4381 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
     4441 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC
     4501 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA
     4561 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC
     4621 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG
     4681 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT
     4741 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGGACATATT GTCGTTAGAA CGCGGCTACA
     4801 ATTAATACAT AACCTTATGT ATCATACACA TACGATTTAG GTGACACTAT A
//
