LOCUS 18 9000 bp DNA circular 02-MAR-2016 FEATURES Location/Qualifiers misc_feature 304..820 /note="CMV promoter" Promoter 863..881 /gene="T7 prom" misc_feature 969..1093 /note="attR1" misc_feature 1202..1862 /note="Chloramphenicol resistance" misc_feature 2203..2508 /note="ccdB" misc_feature 2549..2673 /note="attR2" Rep_Origin 4718..5024 /gene="f1 origin" Regulatory_Seq 5134..5369 /gene="SV40ER reg" Promoter 5156..5424 /gene="SV40 prom" Promoter 5276..5477 /gene="SV40 prom" Rep_Origin 5323..5400 /gene="SV40 origin" Marker 5526..6543 /gene="hygroB marker" Promoter 6895..6924 /gene="lac prom" Rep_Origin 7233..7849 /gene="pBR322 origin" Marker 8004..8864 /gene="amp marker" Promoter 8906..8934 /gene="amp prom" Reporter 2741..4387 /gene="fLuc reporter" insert 2736..4387 /source="pGL3-control" /type="BP AttB Insert" source 1..9000 /dnas_title="18 pcDNA31-ccdB-GSlinker-Firefly luciferase" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc 961 agatATCAAC AAGTTTGTAC AAAAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA 1021 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA 1081 TCCAGTCACT ATGGCGGCCG CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC 1141 GTATAATGTG TGGATTTTGA GTTAGGATCC GTCGAGATTT TCAGGAGCTA AGGAAGCTAA 1201 AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA 1261 ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA 1321 TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT 1381 TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG 1441 TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA 1501 AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA 1561 TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA 1621 GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT 1681 GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG 1741 CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTTTGTG ATGGCTTCCA 1801 TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA 1861 AAGATCTGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT GCGTATTTGC GCGCTGATTT 1921 TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT ATGTCAAAAA GAGGTATGCT 1981 ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT ATCAGTTGCT CAAGGCATAT 2041 ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA GAATGAAGCC CGTCGTCTGC 2101 GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC TGAGGTCGCC CGGTTTATTG 2161 AAATGAACGG CTCTTTTGCT GACGAGAACA GGGGCTGGTG AAATGCAGTT TAAGGTTTAC 2221 ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA TATTATTGAC 2281 ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC AGATAAAGTC 2341 TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT GATGACCACC 2401 GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG TGGCTGATCT CAGCCACCGC 2461 GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC 2521 CTTATACACA GCCAGTCTGC AGGTCGACCA TAGTGACTGG ATATGTTGTG TTTTACAGTA 2581 TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA 2641 CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTTGATatc cagcacagtg gcggccgcTC 2701 GAGTGGCTCG GGCTCGACCT CGGGCTCGGG CAAAACCGGT gaagacgcca aaaacataaa 2761 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa 2821 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga 2881 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa 2941 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt 3001 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat 3061 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt 3121 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa 3181 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt 3241 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag 3301 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt 3361 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg 3421 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg 3481 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt 3541 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt 3601 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa 3661 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc 3721 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc 3781 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt 3841 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg 3901 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac 3961 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga 4021 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca 4081 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg 4141 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga 4201 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac 4261 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg 4321 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat 4381 cgccgtgtag tctagaaatt GGCCcgttta aacccgctga tcagcctcga ctgtgccttc 4441 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 4501 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 4561 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 4621 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 4681 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 4741 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 4801 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 4861 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 4921 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 4981 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 5041 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 5101 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 5161 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 5221 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 5281 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 5341 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 5401 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 5461 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg 5521 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac 5581 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat 5641 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat 5701 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt 5761 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg 5821 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat 5881 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga 5941 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat 6001 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag 6061 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc 6121 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg 6181 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct 6241 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg 6301 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac 6361 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga 6421 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc 6481 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag 6541 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 6601 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 6661 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6721 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6781 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa 6841 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 6901 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 6961 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 7021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 7081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 7141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 7201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 7261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 7321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 7381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 7441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 7501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 7561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 7621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 7681 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 7741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag 7801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 7861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 7921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 7981 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 8041 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 8101 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 8161 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 8221 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 8281 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 8341 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 8401 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 8461 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 8521 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 8581 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 8641 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 8701 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 8761 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 8821 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 8881 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 8941 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //