LOCUS       21                      7854 bp    DNA     circular     02-MAR-2016
FEATURES             Location/Qualifiers
     misc_feature    304..820
                     /note="CMV promoter"
     Promoter        863..881
                     /gene="T7 prom"
     misc_feature    969..1093
                     /note="attR1"
     misc_feature    1202..1862
                     /note="Chloramphenicol resistance"
     misc_feature    2203..2508
                     /note="ccdB"
     misc_feature    2549..2673
                     /note="attR2"
     Rep_Origin      3572..3878
                     /gene="f1 origin"
     Regulatory_Seq  3988..4223
                     /gene="SV40ER reg"
     Promoter        4010..4278
                     /gene="SV40 prom"
     Promoter        4130..4331
                     /gene="SV40 prom"
     Rep_Origin      4177..4254
                     /gene="SV40 origin"
     Marker          4380..5397
                     /gene="hygroB marker"
     Promoter        5749..5778
                     /gene="lac prom"
     Rep_Origin      6087..6703
                     /gene="pBR322 origin"
     Marker          6858..7718
                     /gene="amp marker"
     Promoter        7760..7788
                     /gene="amp prom"
     CDS             2741..3253
                     /note="Nanoluc"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDG
                     VTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVT
                     GWRLCERILA"
     source          1..7854
                     /dnas_title="21 pcDNA31-ccdB-GSlinker-Nanoluc"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatATCAAC AAGTTTGTAC AAAAAAGCTG AACGAGAAAC GTAAAATGAT ATAAATATCA
     1021 ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA
     1081 TCCAGTCACT ATGGCGGCCG CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC
     1141 GTATAATGTG TGGATTTTGA GTTAGGATCC GTCGAGATTT TCAGGAGCTA AGGAAGCTAA
     1201 AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA TCCCAATGGC ATCGTAAAGA
     1261 ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT AACCAGACCG TTCAGCTGGA
     1321 TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC AAGTTTTATC CGGCCTTTAT
     1381 TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC CGTATGGCAA TGAAAGACGG
     1441 TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA
     1501 AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA
     1561 TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA
     1621 GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT
     1681 GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG
     1741 CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTTTGTG ATGGCTTCCA
     1801 TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA
     1861 AAGATCTGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT GCGTATTTGC GCGCTGATTT
     1921 TTGCGGTATA AGAATATATA CTGATATGTA TACCCGAAGT ATGTCAAAAA GAGGTATGCT
     1981 ATGAAGCAGC GTATTACAGT GACAGTTGAC AGCGACAGCT ATCAGTTGCT CAAGGCATAT
     2041 ATGATGTCAA TATCTCCGGT CTGGTAAGCA CAACCATGCA GAATGAAGCC CGTCGTCTGC
     2101 GTGCCGAACG CTGGAAAGCG GAAAATCAGG AAGGGATGGC TGAGGTCGCC CGGTTTATTG
     2161 AAATGAACGG CTCTTTTGCT GACGAGAACA GGGGCTGGTG AAATGCAGTT TAAGGTTTAC
     2221 ACCTATAAAA GAGAGAGCCG TTATCGTCTG TTTGTGGATG TACAGAGTGA TATTATTGAC
     2281 ACGCCCGGGC GACGGATGGT GATCCCCCTG GCCAGTGCAC GTCTGCTGTC AGATAAAGTC
     2341 TCCCGTGAAC TTTACCCGGT GGTGCATATC GGGGATGAAA GCTGGCGCAT GATGACCACC
     2401 GATATGGCCA GTGTGCCGGT CTCCGTTATC GGGGAAGAAG TGGCTGATCT CAGCCACCGC
     2461 GAAAATGACA TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC
     2521 CTTATACACA GCCAGTCTGC AGGTCGACCA TAGTGACTGG ATATGTTGTG TTTTACAGTA
     2581 TTATGTAGTC TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA
     2641 CGTTTCTCGT TCAGCTTTCT TGTACAAAGT GGTTGATatc cagcacagtg gcggccgcTC
     2701 GAGTGGCTCG GGCTCGACCT CGGGCTCGGG CAAAACCGGT GTCTTCACAC TCGAAGATTT
     2761 CGTTGGGGAC TGGCGACAGA CAGCCGGCTA CAACCTGGAC CAAGTCCTTG AACAGGGAGG
     2821 TGTGTCCAGT TTGTTTCAGA ATCTCGGGGT GTCCGTAACT CCGATCCAAA GGATTGTCCT
     2881 GAGCGGTGAA AATGGGCTGA AGATCGACAT CCATGTCATC ATCCCGTATG AAGGTCTGAG
     2941 CGGCGACCAA ATGGGCCAGA TCGAAAAAAT TTTTAAGGTG GTGTACCCTG TGGATGATCA
     3001 TCACTTTAAG GTGATCCTGC ACTATGGCAC ACTGGTAATC GACGGGGTTA CGCCGAACAT
     3061 GATCGACTAT TTCGGACGGC CGTATGAAGG CATCGCCGTG TTCGACGGCA AAAAGATCAC
     3121 TGTAACAGGG ACCCTGTGGA ACGGCAACAA AATTATCGAC GAGCGCCTGA TCAACCCCGA
     3181 CGGCTCCCTG CTGTTCCGAG TAACCATCAA CGGAGTGACC GGCTGGCGGC TGTGCGAACG
     3241 CATTCTGGCG TAAGGGCCcg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3301 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3361 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3421 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3481 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3541 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3601 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3661 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3721 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3781 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3841 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3901 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3961 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4021 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4081 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4141 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4261 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4321 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa
     4381 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     4441 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     4501 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     4561 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     4621 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     4681 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     4741 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     4801 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     4861 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     4921 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     4981 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     5041 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     5101 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     5161 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     5221 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     5281 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     5341 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     5401 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5461 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5521 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5581 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5641 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5701 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5761 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5821 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5881 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5941 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6001 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6061 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6121 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6181 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6241 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6301 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6361 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6421 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6481 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6541 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6601 agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag
     6661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7141 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7201 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7261 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7321 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7381 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7441 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7501 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7561 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7621 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7681 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7801 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//

