LOCUS       40                      8936 bp    DNA     circular     02-MAR-2016
FEATURES             Location/Qualifiers
     promoter        304..888
                     /note="CMV promoter"
     misc_recomb     2606..2730
                     /label=attR1
                     /note="attR1 site"
     gene            2839..3498
                     /note="Chloramphenicol resistance gene (CmR)"
     misc_feature    3840..4145
                     /label=ccdB
     misc_feature    4186..4310
                     /label=attR2
     Rep_Origin      4654..4960
                     /gene="f1 origin"
     Regulatory_Seq  5070..5305
                     /gene="SV40ER reg"
     Promoter        5092..5360
                     /gene="SV40 prom"
     Promoter        5212..5413
                     /gene="SV40 prom"
     Rep_Origin      5259..5336
                     /gene="SV40 origin"
     Marker          5462..6479
                     /gene="hygroB marker"
     Promoter        6831..6860
                     /gene="lac prom"
     Rep_Origin      7169..7785
                     /gene="pBR322 origin"
     Marker          7940..8800
                     /gene="amp marker"
     Promoter        8842..8870
                     /gene="amp prom"
     CDS             2561..2605
                     /note="GS linker"
                     /translation="SGGRSGSTSGSG"
     Reporter        911..2560
                     /gene="fLuc reporter"
     insert          911..2560
                     /source="pGL3-control"
                     /type="BP AttB Insert"
     source          1..8936
                     /dnas_title="40 pcDNA31-Firefly-GS-ccdB"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac GTATTAGTCA
      601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
      661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
      721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
      781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG
      841 CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC TAGAGGATCC
      901 AGCCGCCACC atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      961 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
     1021 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
     1081 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
     1141 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
     1201 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
     1261 tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt
     1321 gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga
     1381 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
     1441 tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa
     1501 ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt
     1561 gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat
     1621 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat
     1681 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct
     1741 tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa
     1801 aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc
     1861 tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag
     1921 gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga
     1981 taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga
     2041 taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat
     2101 tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg
     2161 gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg
     2221 cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat
     2281 cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc
     2341 cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga
     2401 gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt
     2461 gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga
     2521 gatcctcata aaggccaaga agggcggaaa gatcgccgtg AGTGGCGGCC GCTCGAGTGG
     2581 CTCGGGCTCG ACCTCGGGCT CGGGCACAAG TTTGTACAAA AAAGCTGAAC GAGAAACGTA
     2641 AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA
     2701 CTGTAAAACA CAACATATCC AGTCACTATG GCGGCCGCAT TAGGCACCCC AGGCTTTACA
     2761 CTTTATGCTT CCGGCTCGTA TAATGTGTGG ATTTTGAGTT AGGATCCGTC GAGATTTTCA
     2821 GGAGCTAAGG AAGCTAAAAT GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC
     2881 CAATGGCATC GTAAAGAACA TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC
     2941 CAGACCGTTC AGCTGGATAT TACGGCCTTT TTAAAGACCG TAAAGAAAAA TAAGCACAAG
     3001 TTTTATCCGG CCTTTATTCA CATTCTTGCC CGCCTGATGA ATGCTCATCC GGAATTCCGT
     3061 ATGGCAATGA AAGACGGTGA GCTGGTGATA TGGGATAGTG TTCACCCTTG TTACACCGTT
     3121 TTCCATGAGC AAACTGAAAC GTTTTCATCG CTCTGGAGTG AATACCACGA CGATTTCCGG
     3181 CAGTTTCTAC ACATATATTC GCAAGATGTG GCGTGTTACG GTGAAAACCT GGCCTATTTC
     3241 CCTAAAGGGT TTATTGAGAA TATGTTTTTC GTCTCAGCCA ATCCCTGGGT GAGTTTCACC
     3301 AGTTTTGATT TAAACGTGGC CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC
     3361 AAATATTATA CGCAAGGCGA CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC
     3421 GTCTGTGATG GCTTCCATGT CGGCAGAATG CTTAATGAAT TACAACAGTA CTGCGATGAG
     3481 TGGCAGGGCG GGGCGTAAAG ATCTGGATCC GGCTTACTAA AAGCCAGATA ACAGTATGCG
     3541 TATTTGcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     3601 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     3661 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     3721 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     3781 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     3841 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     3901 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     3961 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     4021 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     4081 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     4141 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     4201 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     4261 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt TGCTCTATAG
     4321 TAActcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4381 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4441 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4501 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4561 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4621 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4681 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4741 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4801 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4861 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4921 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4981 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5041 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     5101 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5161 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5221 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5281 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5341 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5401 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgatga
     5461 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     5521 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     5581 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     5641 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     5701 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     5761 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     5821 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     5881 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     5941 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     6001 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     6061 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     6121 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     6181 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     6241 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     6301 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     6361 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     6421 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     6481 agcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6541 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6601 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6661 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6721 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6841 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6901 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6961 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7021 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7081 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
     7741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8401 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8461 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8521 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8581 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8641 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8701 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8761 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8821 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8881 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//

