LOCUS 41 7799 bp DNA circular 02-MAR-2016 FEATURES Location/Qualifiers promoter 304..888 /note="CMV promoter" misc_recomb 1469..1593 /label=attR1 /note="attR1 site" gene 1702..2361 /note="Chloramphenicol resistance gene (CmR)" misc_feature 2703..3008 /label=ccdB misc_feature 3049..3173 /label=attR2 Rep_Origin 3517..3823 /gene="f1 origin" Regulatory_Seq 3933..4168 /gene="SV40ER reg" Promoter 3955..4223 /gene="SV40 prom" Promoter 4075..4276 /gene="SV40 prom" Rep_Origin 4122..4199 /gene="SV40 origin" Marker 4325..5342 /gene="hygroB marker" Promoter 5694..5723 /gene="lac prom" Rep_Origin 6032..6648 /gene="pBR322 origin" Marker 6803..7663 /gene="amp marker" Promoter 7705..7733 /gene="amp prom" CDS 1424..1468 /note="GS linker" /translation="SGGRSSGSGSTSGSG" CDS 911..1423 /note="Nanoluc" /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQ RIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVID GVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGV TGWRLCERILA" source 1..7799 /dnas_title="41 pcDNA31-Nanoluc-GS-ccdB" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG 841 CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC TAGAGGATCC 901 AGCCGCCACC ATGGTCTTCA CACTCGAAGA TTTCGTTGGG GACTGGCGAC AGACAGCCGG 961 CTACAACCTG GACCAAGTCC TTGAACAGGG AGGTGTGTCC AGTTTGTTTC AGAATCTCGG 1021 GGTGTCCGTA ACTCCGATCC AAAGGATTGT CCTGAGCGGT GAAAATGGGC TGAAGATCGA 1081 CATCCATGTC ATCATCCCGT ATGAAGGTCT GAGCGGCGAC CAAATGGGCC AGATCGAAAA 1141 AATTTTTAAG GTGGTGTACC CTGTGGATGA TCATCACTTT AAGGTGATCC TGCACTATGG 1201 CACACTGGTA ATCGACGGGG TTACGCCGAA CATGATCGAC TATTTCGGAC GGCCGTATGA 1261 AGGCATCGCC GTGTTCGACG GCAAAAAGAT CACTGTAACA GGGACCCTGT GGAACGGCAA 1321 CAAAATTATC GACGAGCGCC TGATCAACCC CGACGGCTCC CTGCTGTTCC GAGTAACCAT 1381 CAACGGAGTG ACCGGCTGGC GGCTGTGCGA ACGCATTCTG GCGAGTGGCG GCCGCTCGAG 1441 TGGCTCGGGC TCGACCTCGG GCTCGGGCAC AAGTTTGTAC AAAAAAGCTG AACGAGAAAC 1501 GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA 1561 ATACTGTAAA ACACAACATA TCCAGTCACT ATGGCGGCCG CATTAGGCAC CCCAGGCTTT 1621 ACACTTTATG CTTCCGGCTC GTATAATGTG TGGATTTTGA GTTAGGATCC GTCGAGATTT 1681 TCAGGAGCTA AGGAAGCTAA AATGGAGAAA AAAATCACTG GATATACCAC CGTTGATATA 1741 TCCCAATGGC ATCGTAAAGA ACATTTTGAG GCATTTCAGT CAGTTGCTCA ATGTACCTAT 1801 AACCAGACCG TTCAGCTGGA TATTACGGCC TTTTTAAAGA CCGTAAAGAA AAATAAGCAC 1861 AAGTTTTATC CGGCCTTTAT TCACATTCTT GCCCGCCTGA TGAATGCTCA TCCGGAATTC 1921 CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC 1981 GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC 2041 CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT 2101 TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC 2161 ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG 2221 GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT 2281 GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT 2341 GAGTGGCAGG GCGGGGCGTA AAGATCTGGA TCCGGCTTAC TAAAAGCCAG ATAACAGTAT 2401 GCGTATTTGc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt 2461 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct 2521 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca 2581 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc 2641 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg 2701 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg 2761 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac 2821 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa 2881 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag 2941 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 3001 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg 3061 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata 3121 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtTGCTCTA 3181 TAGTAActcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct 3241 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 3301 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 3361 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 3421 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 3481 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 3541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 3601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 3661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 3721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 3781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 3841 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 3901 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 3961 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4021 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4081 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 4141 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 4201 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4261 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga 4321 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca 4381 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg 4441 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc 4501 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg 4561 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc 4621 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg 4681 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa 4741 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc 4801 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc 4861 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct 4921 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga 4981 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt 5041 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc 5101 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg 5161 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag 5221 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct 5281 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg 5341 aatagcacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 5401 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 5461 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 5521 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 5581 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 5641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 5701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 5761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 5821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 5881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 6001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 6061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 6481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 6541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc 6601 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6661 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 6721 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6781 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6841 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 6901 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 6961 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 7021 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 7081 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 7141 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 7201 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 7261 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 7321 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 7381 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 7441 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 7501 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 7561 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 7621 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 7681 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 7741 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc //