LOCUS       lenti-Cas9-VQR         12860 bp    DNA     circular SYN 14-Jan-2017
DEFINITION  -
ACCESSION   -
KEYWORDS    -
SOURCE      -
FEATURES             Location/Qualifiers
     misc_feature    252..281
                     /note="/pair="""
                     /note="/primer=""ACTAGTTATTAATAGTAATCAATTACGGGG"
                     /note="/current=0"""
     promoter        254..844
                     /note="CMV"
     misc_feature    318..605
                     /label=CAG_enhancer
     misc_feature    319..606
                     /label=CAG_enhancer
     exon            404..423
                     /note="XPR101_F7"
     misc_feature    649..671
                     /note="CMV promoter seq F"
     misc_feature    681..827
                     /note="CMVie"
     exon            772..792
                     /note="CMV forward"
     repeat_region   772..792
                     /note="CMV_forward"
     primer_bind     789..808
                     /note="pLVX-Puro-F"
     LTR             828..1016
                     /note="5' LTR (R and U5 portions; U3 was replaced by the
                     CMV promoter)"
     misc_feature    828..1016
                     /note="5'LTR"
     exon            828..1016
                     /note="5'LTR"
     misc_feature    835..1528
                     /note="full match to pHR and LKO"
     misc_binding    1017..1034
                     /label=PBS
     exon            1067..1204
                     /note="psi"
     LTR             1126..1170
                     /note="HIV-1 psi pack"
     exon            1171..1535
                     /note="gag"
     misc_feature    1171..1535
                     /note="gag"
     misc_feature    1529..2390
                     /note="full match to pHR' and LKO"
     LTR             1680..1913
                     /note="rre"
     misc_binding    1680..1913
                     /label=RRE
     misc_feature    1681..1922
                     /label=RRE
     exon            1681..1922
                     /note="RRE"
     primer_bind     1746..1765
                     /note="pLVX-Puro-F2"
     primer_bind     1835..1854
                     /note="pLVX-Puro-R2"
     primer_bind     1932..1951
                     /note="pLVX-F3"
     exon            1932..1951
                     /note="XPR101_F9"
     primer_bind     2018..2037
                     /note="pLVX-R3"
     exon            2203..2224
                     /note="U6F_tandem_F"
     misc_feature    complement(2375..2396)
                     /note="/pair="""
                     /note="/primer=""AGTGCCGATCCGTTCACTAATC"
                     /note="/current=0"""
     misc_feature    2397..2413
                     /note="/pair="""
                     /note="/primer=""GCGTGCGCCAATTCTGC"
                     /note="/current=0"""
     repeat_region   2408..2427
                     /note="XPR101_F9B"
     primer_bind     2410..2424
                     /note="Taka-RV"
     exon            2410..2424
                     /note="sgRNA_tandem_R"
     exon            2415..2436
                     /note="tandemRV"
     exon            2418..2439
                     /note="tandem_R"
     exon            2440..2557
                     /note="cPPT"
     misc_feature    2440..2557
                     /label=cPPT
     misc_feature    2441..2564
                     /label=cPPT
                     /note="/CTS\(DNA\flap)"
     primer_bind     2466..2485
                     /note="pLTG-F"
     repeat_region   2466..2485
                     /note="pLTG_F"
     primer_bind     2469..2493
                     /note="Priming site R"
     primer_bind     2478..2522
                     /note="LC_readout_R"
     promoter        2608..2864
     exon            2608..2863
                     /note="EFS-NS"
     frag            2608..2863
                     /date="27-Feb-2015"
                     /note="2967 to 3222 of pHKO_23-pFUGW-U6-BsmBI (no
                     spacer)-chRNA(+85)-EFS-Cas9-2A-GFP-pA"
     repeat_region   2608..2627
                     /note="EFS_NS_F"
     primer_bind     2614..2633
                     /note="EF1a_R"
     misc_feature    complement(2692..2709)
                     /note="/dnas_title=""TCAATTGCCGACCCCTCC"
                     /note="/pair="""
                     /note="/primer=""TCAATTGCCGACCCCTCC"
                     /note="/current=1"""
     misc_feature    complement(2699..2716)
                     /note="XRP008 partial binding site"
     repeat_region   2751..2770
                     /note="EFS_R"
     primer_bind     2804..2823
                     /note="EF1aR"
     5'UTR           2830..2862
                     /note="5'UTR exon1"
     repeat_region   2844..2863
                     /note="EFS_NS_R"
     repeat_region   2860..2967
                     /note="EFS-NS-XbaI-Cas9F"
     exon            2860..2967
                     /note="EFS-NS-XbaI-Cas9F"
     frag            2860..2967
                     /date="28-Oct-2015"
                     /note="1 to 108 of EFS-NS-XbaI-Cas9F"
     misc_feature    2887..6990
                     /note="hSpCas9"
     gene            2887..7038
     exon            2887..6990
                     /note="hSpCas9"
     frag            2890..6990
                     /date="14-Jul-2016"
                     /note="2890 to 6990 of pXPR_101"
     CDS             2890..6990
                     /note="S.pyo Cas9"
     repeat_region   3076..3095
                     /note="Cas9_R(not pXPR101_Cas9_R!)"
     exon            3271..3290
                     /note="Cas9_F2"
     exon            3791..3810
                     /note="Cas9_F3"
     repeat_region   4279..4298
                     /note="Cas9_R"
     exon            4279..4298
                     /note="pHKO9_Cas9_R"
     exon            4311..4330
                     /note="Cas9_F4"
     exon            4809..4828
                     /note="Cas9_F5"
     misc_binding    5037..5072
     primer_bind     5291..5310
     exon            5356..5375
                     /note="Cas9_F6"
     exon            5810..5829
                     /note="Cas9_F7"
     primer_bind     6289..6291
                     /note="D1135V"
     exon            6301..6320
                     /note="Cas9_F8"
     primer_bind     6889..6891
                     /note="R1335Q"
     primer_bind     6895..6897
                     /note="T1337R"
     primer_bind     6952..6971
                     /note="Cas9_F9"
     frag            6952..7077
                     /date="28-Oct-2015"
                     /note="1 to 126 of P2A-BamHI-FLAG-NLS-Cas9-R"
     exon            6952..7077
                     /note="P2A-BamHI-FLAG-NLS-Cas9-R"
     repeat_region   6952..7077
                     /note="P2A-BamHI-FLAG-NLS-Cas9-R"
     exon            6991..7062
                     /note="NLS-FLAG_from_101-Cas9"
     misc_feature    6991..7038
                     /note="NLS"
     misc_feature    7039..7062
     misc_feature    7062..7128
     repeat_region   7063..7128
                     /note="P2A"
     exon            7063..7128
                     /note="P2A"
     frag            <7063..>7131
                     /date="04-Mar-2015"
                     /note="110995318 to 110996883 of mHbb cluster b1-2A-GFP
                     [Split]"
     CDS             7129..7524
                     /note="BlastR"
     gene            7129..7527
     primer_bind     7370..7389
                     /note="BLAST_R"
     repeat_region   7370..7389
                     /note="BLAST_R"
     exon            7370..7389
                     /note="BLAST_R"
     misc_feature    7542..8150
                     /label=WPRE
                     /note="/dnas_title=""WPRE"
                     /note="/vntifkey=""21"""
     frag            7552..8140
                     /note="1964 to 2552 of pLKO_TRC047"
     misc_binding    7552..8140
                     /note="WPRE"
     exon            7552..8140
                     /note="WPRE"
     exon            7554..8141
                     /note="WPRE"
     primer_bind     7659..7678
                     /note="WPRE_R"
     exon            8234..8253
                     /note="3'LTR_F"
     misc_feature    8328..8343
                     /note="cPPT/FLAP"
     LTR             8345..8846
                     /note="3'LTR"
     misc_feature    8345..8846
                     /note="3'SIN-LTR"
     repeat_region   8351..8370
                     /note="3'LTR_R"
     exon            8658..8846
                     /note="5'LTR"
     frag            8846..9086
                     /note="1 to 241 of Untitled"
     exon            8846..9086
                     /note="pA"
     misc_feature    8852..9086
                     /note="polyA?"
     polyA_signal    8870..9123
                     /note="BGH polyA"
     repeat_region   8871..8888
                     /note="BGH_rev"
     primer_bind     8999..9017
                     /note="PPD132"
     primer_bind     9089..9108
                     /note="Neo-F6"
     rep_origin      9147..9575
                     /note="F1 ori"
     exon            9164..9470
                     /note="f1ori"
     primer_bind     9313..9330
                     /note="PPD109"
     primer_bind     9356..9378
                     /note="HX56"
     rep_origin      9602..9911
                     /note="SV40 rep"
     exon            9661..9680
                     /note="XPR101_F2"
     promoter        9733..9912
                     /note="/label=""SV40_promoter"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     promoter        9734..9913
                     /note="/label=""SV40_promoter"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
                     NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"""
     misc_feature    9831..9850
                     /label=SV40pro_F_primer
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
                     NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"""
     CDS             10032..10406
                     /note="BleomycinR"
     exon            10399..10418
                     /note="XPR101_F3"
     polyA_signal    10536..10666
                     /note="SV40 polyadenylation signal"
     exon            10536..10666
                     /note="SV40 polyA"
     misc_feature    10627..10646
                     /label=EBV_rev_primer
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
                     NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"""
     misc_feature    10696..10943
                     /note="lac operon sequence"
     misc_feature    complement(10696..10925)
                     /note="Lac-operon"
     promoter        complement(10702..10720)
                     /note="/note=""/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKR
                     RVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELK
                     NSAVSLLNATAIAVAEGTDRVIEVVQGACRAIRHIPRRIRQGLERILL*"""
                     /note="/label=M13_reverse_primer"
     primer_bind     10706..10722
                     /note="binding site of pUC/M13 reverse sequencing primer"
     primer_bind     complement(10706..10727)
                     /note="pUC/M13 reverse sequencing primer"
     promoter        complement(10711..10832)
                     /note="Plac"
     primer_bind     complement(10719..10742)
                     /note="Ptac forward sequencing primer (NEB #1260,
                     discontinued)"
     protein_bind    complement(10726..10750)
                     /bound_moiety="LacI"
                     /note="lac operator"
     protein_bind    complement(10804..10816)
                     /bound_moiety="catabolite activator protein (CAP)"
                     /note="CAP protein binding site"
     misc_feature    complement(10925..10988)
                     /citation=[5]
                     /note="L-strand Y effector site"
     rep_origin      10991..11606
                     /label=pUC\Origin
                     /note="vntifkey=33"
     exon            complement(10991..11606)
                     /note="pUC Ori"
     rep_origin      complement(11049..11722)
                     /label=pUC\origin
     repeat_region   11049..11722
                     /note="pUC Ori"
     misc_feature    complement(11049..11722)
                     /note="pUC origin"
     exon            complement(11052..11719)
                     /note="pUC ori"
     exon            11071..11090
                     /note="XPR101_F4"
     misc_RNA        complement(11108..11660)
                     /note="RNAII transcript (complementary strand)"
     rep_origin      11108..11696
                     /note="pMB1 origin of replication (counterclockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     primer_bind     11291..11310
                     /note="pLVX-Puro-F5"
     exon            11295..11520
                     /note="ColE1"
     misc_feature    11295..11520
                     /note="ColE1 rep ori"
     primer_bind     11413..11432
                     /note="pLVX-Puro-F1"
     primer_bind     11417..11436
                     /note="pLVX-Puro-R5"
     primer_bind     11479..11498
                     /note="pLVX-Puro-R1"
     misc_RNA        11550..11657
                     /note="RNAI transcript"
     repeat_region   11722..11759
                     /note="corresponds to one of the 38bp repeats found in Tn3
                     (bp 1-38 and complement (4920-4957))"
                     /rpt_type=inverted
     exon            11728..11747
                     /note="XPR101_F5"
     misc_feature    complement(11732..12538)
                     /label=AMP\r
                     /note="vntifkey=21"
     exon            complement(11732..12538)
                     /note="AmpR"
     gene            complement(11867..12727)
                     /gene="bla"
     repeat_region   11867..12860
                     /note="AmpR [Split]"
     exon            complement(11867..12727)
                     /note="AmpR"
     misc_difference complement(11867..12727)
                     /note="AmpR"
     CDS             complement(11867..12860)
                     /note="AmpR [Split]"
     misc_feature    complement(11867..12727)
                     /label=Amp(R)
     primer_bind     12119..12138
                     /note="pLVX-Puro-F4"
     primer_bind     12203..12222
                     /note="pLVX-Puro-R4"
     misc_binding    12444..12479
     exon            12490..12509
                     /note="XPR101_F6"
     sig_peptide     complement(12659..12727)
                     /gene="bla"
                     /note="required for secretion to the periplasm"
                     /note="cleaved off to form the mature beta-lactamase
                     protein."
     promoter        complement(12728..12832)
                     /gene="bla"
                     /note="AmpR promoter"
     exon            complement(12769..12797)
                     /note="AmpRp"
BASE COUNT      3363 A      3278 C      3450 G      2769 T         0 OTHER
ORIGIN      ?
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
      241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
      301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
      361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
      421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
      481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
      541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
      601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
      661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
      721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
      781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
      841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
      901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
      961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
     1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
     1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
     1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
     1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
     1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
     1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
     1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
     1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
     1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG
     1561 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA
     1621 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA
     1681 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG
     1741 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG
     1801 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG
     1861 CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT
     1921 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT
     1981 AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT
     2041 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG
     2101 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA
     2161 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA
     2221 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA
     2281 TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA
     2341 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT
     2401 GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT
     2461 TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA
     2521 AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG
     2581 AGATCCAGTT TGGTTAATTA AGCTAGCTAG GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT
     2641 GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC
     2701 GGCAATTGAT CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG
     2761 TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC
     2821 GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGACCGGTT CTAGAGCGCT
     2881 GCCACCATGG ACAAGAAGTA CAGCATCGGC CTGGACATCG GCACCAACTC TGTGGGCTGG
     2941 GCCGTGATCA CCGACGAGTA CAAGGTGCCC AGCAAGAAAT TCAAGGTGCT GGGCAACACC
     3001 GACCGGCACA GCATCAAGAA GAACCTGATC GGAGCCCTGC TGTTCGACAG CGGCGAAACA
     3061 GCCGAGGCCA CCCGGCTGAA GAGAACCGCC AGAAGAAGAT ACACCAGACG GAAGAACCGG
     3121 ATCTGCTATC TGCAAGAGAT CTTCAGCAAC GAGATGGCCA AGGTGGACGA CAGCTTCTTC
     3181 CACAGACTGG AAGAGTCCTT CCTGGTGGAA GAGGATAAGA AGCACGAGCG GCACCCCATC
     3241 TTCGGCAACA TCGTGGACGA GGTGGCCTAC CACGAGAAGT ACCCCACCAT CTACCACCTG
     3301 AGAAAGAAAC TGGTGGACAG CACCGACAAG GCCGACCTGC GGCTGATCTA TCTGGCCCTG
     3361 GCCCACATGA TCAAGTTCCG GGGCCACTTC CTGATCGAGG GCGACCTGAA CCCCGACAAC
     3421 AGCGACGTGG ACAAGCTGTT CATCCAGCTG GTGCAGACCT ACAACCAGCT GTTCGAGGAA
     3481 AACCCCATCA ACGCCAGCGG CGTGGACGCC AAGGCCATCC TGTCTGCCAG ACTGAGCAAG
     3541 AGCAGACGGC TGGAAAATCT GATCGCCCAG CTGCCCGGCG AGAAGAAGAA TGGCCTGTTC
     3601 GGAAACCTGA TTGCCCTGAG CCTGGGCCTG ACCCCCAACT TCAAGAGCAA CTTCGACCTG
     3661 GCCGAGGATG CCAAACTGCA GCTGAGCAAG GACACCTACG ACGACGACCT GGACAACCTG
     3721 CTGGCCCAGA TCGGCGACCA GTACGCCGAC CTGTTTCTGG CCGCCAAGAA CCTGTCCGAC
     3781 GCCATCCTGC TGAGCGACAT CCTGAGAGTG AACACCGAGA TCACCAAGGC CCCCCTGAGC
     3841 GCCTCTATGA TCAAGAGATA CGACGAGCAC CACCAGGACC TGACCCTGCT GAAAGCTCTC
     3901 GTGCGGCAGC AGCTGCCTGA GAAGTACAAA GAGATTTTCT TCGACCAGAG CAAGAACGGC
     3961 TACGCCGGCT ACATTGACGG CGGAGCCAGC CAGGAAGAGT TCTACAAGTT CATCAAGCCC
     4021 ATCCTGGAAA AGATGGACGG CACCGAGGAA CTGCTCGTGA AGCTGAACAG AGAGGACCTG
     4081 CTGCGGAAGC AGCGGACCTT CGACAACGGC AGCATCCCCC ACCAGATCCA CCTGGGAGAG
     4141 CTGCACGCCA TTCTGCGGCG GCAGGAAGAT TTTTACCCAT TCCTGAAGGA CAACCGGGAA
     4201 AAGATCGAGA AGATCCTGAC CTTCCGCATC CCCTACTACG TGGGCCCTCT GGCCAGGGGA
     4261 AACAGCAGAT TCGCCTGGAT GACCAGAAAG AGCGAGGAAA CCATCACCCC CTGGAACTTC
     4321 GAGGAAGTGG TGGACAAGGG CGCTTCCGCC CAGAGCTTCA TCGAGCGGAT GACCAACTTC
     4381 GATAAGAACC TGCCCAACGA GAAGGTGCTG CCCAAGCACA GCCTGCTGTA CGAGTACTTC
     4441 ACCGTGTATA ACGAGCTGAC CAAAGTGAAA TACGTGACCG AGGGAATGAG AAAGCCCGCC
     4501 TTCCTGAGCG GCGAGCAGAA AAAGGCCATC GTGGACCTGC TGTTCAAGAC CAACCGGAAA
     4561 GTGACCGTGA AGCAGCTGAA AGAGGACTAC TTCAAGAAAA TCGAGTGCTT CGACTCCGTG
     4621 GAAATCTCCG GCGTGGAAGA TCGGTTCAAC GCCTCCCTGG GCACATACCA CGATCTGCTG
     4681 AAAATTATCA AGGACAAGGA CTTCCTGGAC AATGAGGAAA ACGAGGACAT TCTGGAAGAT
     4741 ATCGTGCTGA CCCTGACACT GTTTGAGGAC AGAGAGATGA TCGAGGAACG GCTGAAAACC
     4801 TATGCCCACC TGTTCGACGA CAAAGTGATG AAGCAGCTGA AGCGGCGGAG ATACACCGGC
     4861 TGGGGCAGGC TGAGCCGGAA GCTGATCAAC GGCATCCGGG ACAAGCAGTC CGGCAAGACA
     4921 ATCCTGGATT TCCTGAAGTC CGACGGCTTC GCCAACAGAA ACTTCATGCA GCTGATCCAC
     4981 GACGACAGCC TGACCTTTAA AGAGGACATC CAGAAAGCCC AGGTGTCCGG CCAGGGCGAT
     5041 AGCCTGCACG AGCACATTGC CAATCTGGCC GGCAGCCCCG CCATTAAGAA GGGCATCCTG
     5101 CAGACAGTGA AGGTGGTGGA CGAGCTCGTG AAAGTGATGG GCCGGCACAA GCCCGAGAAC
     5161 ATCGTGATCG AAATGGCCAG AGAGAACCAG ACCACCCAGA AGGGACAGAA GAACAGCCGC
     5221 GAGAGAATGA AGCGGATCGA AGAGGGCATC AAAGAGCTGG GCAGCCAGAT CCTGAAAGAA
     5281 CACCCCGTGG AAAACACCCA GCTGCAGAAC GAGAAGCTGT ACCTGTACTA CCTGCAGAAT
     5341 GGGCGGGATA TGTACGTGGA CCAGGAACTG GACATCAACC GGCTGTCCGA CTACGATGTG
     5401 GACCATATCG TGCCTCAGAG CTTTCTGAAG GACGACTCCA TCGACAACAA GGTGCTGACC
     5461 AGAAGCGACA AGAACCGGGG CAAGAGCGAC AACGTGCCCT CCGAAGAGGT CGTGAAGAAG
     5521 ATGAAGAACT ACTGGCGGCA GCTGCTGAAC GCCAAGCTGA TTACCCAGAG AAAGTTCGAC
     5581 AATCTGACCA AGGCCGAGAG AGGCGGCCTG AGCGAACTGG ATAAGGCCGG CTTCATCAAG
     5641 AGACAGCTGG TGGAAACCCG GCAGATCACA AAGCACGTGG CACAGATCCT GGACTCCCGG
     5701 ATGAACACTA AGTACGACGA GAATGACAAG CTGATCCGGG AAGTGAAAGT GATCACCCTG
     5761 AAGTCCAAGC TGGTGTCCGA TTTCCGGAAG GATTTCCAGT TTTACAAAGT GCGCGAGATC
     5821 AACAACTACC ACCACGCCCA CGACGCCTAC CTGAACGCCG TCGTGGGAAC CGCCCTGATC
     5881 AAAAAGTACC CTAAGCTGGA AAGCGAGTTC GTGTACGGCG ACTACAAGGT GTACGACGTG
     5941 CGGAAGATGA TCGCCAAGAG CGAGCAGGAA ATCGGCAAGG CTACCGCCAA GTACTTCTTC
     6001 TACAGCAACA TCATGAACTT TTTCAAGACC GAGATTACCC TGGCCAACGG CGAGATCCGG
     6061 AAGCGGCCTC TGATCGAGAC AAACGGCGAA ACCGGGGAGA TCGTGTGGGA TAAGGGCCGG
     6121 GATTTTGCCA CCGTGCGGAA AGTGCTGAGC ATGCCCCAAG TGAATATCGT GAAAAAGACC
     6181 GAGGTGCAGA CAGGCGGCTT CAGCAAAGAG TCTATCCTGC CCAAGAGGAA CAGCGATAAG
     6241 CTGATCGCCA GAAAGAAGGA CTGGGACCCT AAGAAGTACG GCGGCTTCGT GAGCCCCACC
     6301 GTGGCCTATT CTGTGCTGGT GGTGGCCAAA GTGGAAAAGG GCAAGTCCAA GAAACTGAAG
     6361 AGTGTGAAAG AGCTGCTGGG GATCACCATC ATGGAAAGAA GCAGCTTCGA GAAGAATCCC
     6421 ATCGACTTTC TGGAAGCCAA GGGCTACAAA GAAGTGAAAA AGGACCTGAT CATCAAGCTG
     6481 CCTAAGTACT CCCTGTTCGA GCTGGAAAAC GGCCGGAAGA GAATGCTGGC CTCTGCCGGC
     6541 GAACTGCAGA AGGGAAACGA ACTGGCCCTG CCCTCCAAAT ATGTGAACTT CCTGTACCTG
     6601 GCCAGCCACT ATGAGAAGCT GAAGGGCTCC CCCGAGGATA ATGAGCAGAA ACAGCTGTTT
     6661 GTGGAACAGC ACAAGCACTA CCTGGACGAG ATCATCGAGC AGATCAGCGA GTTCTCCAAG
     6721 AGAGTGATCC TGGCCGACGC TAATCTGGAC AAAGTGCTGT CCGCCTACAA CAAGCACCGG
     6781 GATAAGCCCA TCAGAGAGCA GGCCGAGAAT ATCATCCACC TGTTTACCCT GACCAATCTG
     6841 GGAGCCCCTG CCGCCTTCAA GTACTTTGAC ACCACCATCG ACCGGAAGCA GTACAGAAGC
     6901 ACCAAAGAGG TGCTGGACGC CACCCTGATC CACCAGAGCA TCACCGGCCT GTACGAGACA
     6961 CGGATCGACC TGTCTCAGCT GGGAGGCGAC AAGCGACCTG CCGCCACAAA GAAGGCTGGA
     7021 CAGGCTAAGA AGAAGAAAGA TTACAAAGAC GATGACGATA AGGGATCCGG CGCAACAAAC
     7081 TTCTCTCTGC TGAAACAAGC CGGAGATGTC GAAGAGAATC CTGGACCGAT GGCCAAGCCT
     7141 TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC
     7201 ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT
     7261 GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT GCTGGGCACT
     7321 GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG
     7381 GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA TCCTGGGATC
     7441 AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG
     7501 CCCTCTGGTT ATGTGTGGGA GGGCTAAGAA TTCGATATCA AGCTTATCGA TAATCAACCT
     7561 CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG
     7621 CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC
     7681 ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT
     7741 GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC
     7801 ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG
     7861 GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT
     7921 GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT
     7981 GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG
     8041 GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC
     8101 CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA
     8161 GACCTAGAAA AACATGGAGC AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT
     8221 GCCTGGCTAG AAGCACAAGA GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT
     8281 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG
     8341 GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC
     8401 CACACACAAG GCTACTTCCC TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT
     8461 CCACTGACCT TTGGATGGTG CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA
     8521 GCCAATGAAG GAGAGAACAC CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC
     8581 CCGGAGAGAG AAGTATTAGA GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC
     8641 CGAGAGCTGC ATCCGGACTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
     8701 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
     8761 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
     8821 TAGTCAGTGT GGAAAATCTC TAGCAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT
     8881 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA
     8941 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG
     9001 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA
     9061 AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC
     9121 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG
     9181 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT
     9241 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG
     9301 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA
     9361 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC
     9421 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC
     9481 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA
     9541 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA
     9601 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
     9661 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC
     9721 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
     9781 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA
     9841 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
     9901 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAG
     9961 CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC GACAAGGTGA
    10021 GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG
    10081 CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG
    10141 ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG
    10201 TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG
    10261 AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA
    10321 TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC
    10381 ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT
    10441 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC
    10501 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG
    10561 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT
    10621 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT
    10681 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
    10741 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
    10801 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
    10861 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
    10921 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
    10981 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
    11041 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
    11101 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
    11161 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
    11221 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
    11281 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
    11341 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
    11401 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
    11461 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
    11521 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
    11581 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
    11641 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
    11701 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
    11761 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
    11821 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
    11881 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
    11941 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
    12001 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
    12061 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
    12121 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
    12181 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
    12241 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
    12301 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
    12361 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
    12421 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
    12481 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
    12541 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
    12601 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
    12661 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
    12721 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
    12781 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
    12841 CCCGAAAAGT GCCACCTGAC
//
