LOCUS       Exported                7768 bp ds-DNA     circular SYN 24-JAN-2017
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pEFIRES-P-EGFP-ADRP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, January 24, 2017 from SnapGene Viewer 3.1.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        16..1178
                     /note="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          247..1169
                     /note="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     intron          1217..1349
                     /note="chimeric intron"
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1394..1412
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1434..2150
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2172..3485
                     /note="ADRP Homo sapiens"
                     /note="BC005127.2  Homo sapiens adipose 
                     differentiation-related protein"
     misc_feature    3559..4131
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3582..4133
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4133..4735
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     polyA_signal    4969..5103
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5178..5633
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5967..6071
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6072..6932
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7103..7691
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 attcaagctt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
       61 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
      121 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      181 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      241 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
      301 tgcgtgcctt gaattacttc cacctggctc cagtacgtga ttcttgatcc cgagctggag
      361 ccaggggcgg gccttgcgct ttaggagccc cttcgcctcg tgcttgagtt gaggcctggc
      421 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
      481 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
      541 aagatagtct tgtaaatgcg ggccaggatc tgcacactgg tatttcggtt tttgggcccg
      601 cggccggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
      661 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
      721 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
      781 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctcc agggggctca aaatggagga
      841 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
      901 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
      961 acttctggag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     1021 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     1081 tctccttgga atttggcctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     1141 tggttcaaag tttttttctt ccatttcagg tgtcgtgaac acgggctgca gaagttggtc
     1201 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     1261 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     1321 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1381 aggctagagt acttaatacg actcactata ggctagcgct accggtcgcc accatggtga
     1441 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1501 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1561 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1621 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1681 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1741 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1801 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1861 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1921 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1981 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2041 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2101 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     2161 gatctcgagc tatggcatcc gttgcagttg atccacaacc gagtgtggtg actcgggtgg
     2221 tcaacctgcc cttggtgagc tccacgtatg acctcatgtc ctcagcctat ctcagtacaa
     2281 aggaccagta tccctacctg aagtctgtgt gtgagatggc agagaacggt gtgaagacca
     2341 tcacctccgt ggccatgacc agtgctctgc ccatcatcca gaagctagag ccgcaaattg
     2401 cagttgccaa tacctatgcc tgtaaggggc tagacaggat tgaggagaga ctgcctattc
     2461 tgaatcagcc atcaactcag attgttgcca atgccaaagg cgctgtgact ggggcaaaag
     2521 atgctgtgac gactactgtg actggggcca aggattctgt ggccagcacg atcacagggg
     2581 tgatggacaa gaccaaaggg gcagtgactg gcagtgtgga gaagaccaag tctgtggtca
     2641 gtggcagcat taacacagtc ttggggagtc ggatgatgca gctcgtgagc agtggcgtag
     2701 aaaatgcact caccaaatca gagctgttgg tagaacagta cctccctctc actgaggaag
     2761 aactagaaaa agaagcaaaa aaagttgaag gatttgatct ggttcagaag ccaagttatt
     2821 atgttagact gggatccctg tctaccaagc ttcactcccg tgcctaccag caggctctca
     2881 gcagggttaa agaagctaag caaaaaagcc aacagaccat ttctcagctc cattctactg
     2941 ttcacctgat tgaatttgcc aggaagaatg tgtatagtgc caatcagaaa attcaggatg
     3001 ctcaggataa gctctacctc tcatgggtag agtggaaaag gagcattgga tatgatgata
     3061 ctgatgagtc ccactgtgct gagcacattg agtcacgtac tcttgcaatt gcccgcaacc
     3121 tgactcagca gctccagacc acgtgccaca ccctcctgtc caacatccaa ggtgtaccac
     3181 agaacatcca agatcaagcc aagcacatgg gggtgatggc aggcgacatc tactcagtgt
     3241 tccgcaatgc tgcctccttt aaagaagtgt ctgacagcct cctcacttct agcaaggggc
     3301 agctgcagaa aatgaaggaa tctttagatg acgtgatgga ttatcttgtt aacaacacgc
     3361 ccctcaactg gctggtaggt cccttttatc ctcagctgac tgagtctcag aatgctcagg
     3421 accaaggtgc agagatggac aagagcagcc aggagaccca gcgatctgag cataaaactc
     3481 attaagtcga gctcaagctt cgaattcacg cgtggtacct ctagagtcga cccgggcggc
     3541 cgctctagcc caattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     3601 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3661 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3721 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3781 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3841 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3901 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3961 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     4021 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta
     4081 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatgggcac
     4141 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     4201 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     4261 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4321 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4381 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4441 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4501 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4561 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4621 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4681 cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg cctgacgccc
     4741 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct ccgaccgaag
     4801 ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga ctctagctag
     4861 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     4921 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5101 atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5161 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5221 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5281 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5341 tttagggttc cgatttagag ctttacggca cctcgaccgc aaaaaacttg atttgggtga
     5401 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5461 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5521 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5581 gatttaacaa atatttaacg cgaattttaa caaaatatta acgtttacaa tttcgcctga
     5641 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     5701 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     5761 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     5821 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     5881 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5941 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6001 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6061 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6121 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6181 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6241 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6301 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6361 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6421 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6481 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6541 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6601 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6661 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6721 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6781 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6841 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6901 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6961 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7021 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7081 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7141 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7201 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7261 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7321 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7381 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7441 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7501 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7561 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7621 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7681 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7741 ttgctcacat ggctcgacag atccccca
//
