LOCUS psglessClonePX 7409 bp ds-DNA circular 12-JAN-2017 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 35..617 /label=CMV promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 626..1488 /label=filler insert /ApEinfo_fwdcolor=#8631ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1489..1567 /label=polyA signal /ApEinfo_fwdcolor=#ffc87b /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 625..625 /label=NheI RE site (unique in backbone) /ApEinfo_fwdcolor=#768eff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1574..1579 /label=composite RE site /ApEinfo_fwdcolor=#7dff95 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1568..1573 /label=XhoI /ApEinfo_fwdcolor=#de55ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 12..34 /label=p293T-F2seq /ApEinfo_fwdcolor=#d9adff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1679..1701 /label=293T-R2seq /ApEinfo_fwdcolor=#c57aff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 gggccgcgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg 61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc 121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca 181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg 241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg 301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt 361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca 421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg 481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact 541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag 601 ctggtttagt gaaccgtcag atccgCTAGC GAAGTTAGTG ATAATGGGAT TGGTTCTGAT 661 ATTGAGCTGC TTAGAGCAGC TGTTTTGAAA AATGGGGGTG GGTGGCATGG TCTTGGGTGG 721 GTTGGAGAAG GCAAATGGAT TGTGAAGAAA GGTAATGTTA GCTCAGCCGG GAAATGTTTG 781 AGCTGTGATG AGCATTTGGC TTGTGTTGAT ACCAATGAGG TAGAGACTGA AGATTTTGTG 841 AATTCTTTGG TGACTTTGGC TATGGAGAGG AAGGCAAAGA TGAATTCATG CGAGCCTATG 901 GCGGATTTCA GTGAGTTTCA GGAGTGGCTT GAAAAGCATG GAGATTACGA AGCTATACTA 961 GATGGAGCAA ATATTGGGCT CTACCAACAA AATTTTGCAG ATGGTGGTTT CAGTCTACCA 1021 CAGCTTGAAG CTGTAGTGAA GGAACTTTAC AATAAAAGTG GTAGCAAAAA ACAGCCGCTA 1081 ATTCTCTTGC ACAAGAAACG GGTCAATGCG CTTTTAGAAA ACCCGAATCA CCGGAATCTT 1141 GTGGAAGAAT GGATTAATAA CAATGTCCTG TATGCGACTC CACCAGGTTC CAATGATGAT 1201 TGGTATTGGC TCTACGCTGC TGCTAAACTC AAGTGTTTAC TTGTGACTAA TGATGAGATG 1261 AGAGATCACA TTTTTGAACT ATTAAGTAAC AGTTTCTTCC AAAAATGGAA AGAAAGACAT 1321 CAAGTTCGGT TTACCTTTGT GAAAGGCTGT CTCAAGCTTG AAATGCCACC TCCTTTTTCA 1381 GTTGTGATCC AGGAATCGGA GAAAGGATCA TGGCACGTTC CAATCACGAG TCAAGACAAG 1441 GAGGAATCTT TAAGAAGTTG GATGTGCATT ACAAGGCAGA GTTCATAGAA TAAAATATCT 1501 TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGTAT AGCATACATT ATACGAAGTT 1561 ATggatcctc gagcaattcg tcgagggacc taataacttc gtatagcata cattatacga 1621 agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga 1681 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 1741 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 1801 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 1861 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 1921 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 1981 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 2041 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 2101 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 2161 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 2221 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg 2281 tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta 2341 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca 2401 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt 2461 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg 2521 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc 2581 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag 2641 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc 2701 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat 2761 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga 2821 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 2881 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 2941 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc 3001 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3061 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 3181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 3241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 3301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 3361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 3421 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 3481 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 3541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 3601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 3661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 3721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3781 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3841 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3901 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3961 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4021 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4081 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 4141 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 4201 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 4261 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 4321 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 4381 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 4441 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 4501 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 4561 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 4621 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 4681 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 4741 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcttaag 4801 cggtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc 4861 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 4921 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa 4981 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg 5041 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 5101 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 5161 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 5221 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 5281 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 5341 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 5401 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 5461 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 5521 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 5581 gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt 5641 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 5701 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 5761 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg 5821 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac 5881 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa 5941 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg 6001 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata 6061 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg 6121 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca 6181 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca 6241 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa 6301 caaaagtaag accaccgcac agcaagcggc cggccgcgct gatcttcaga cctggaggag 6361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac 6421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag 6481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag 6541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga 6601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca 6661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc 6721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta 6781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca 6841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc 6901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt 6961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg 7021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt 7081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa 7141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg 7201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa 7261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata 7321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg 7381 gacagcagag atccagtttg gttagtacc //