LOCUS       hisSUMO-mZFP57-f        3384 bp ds-DNA     circular SYN 20-MAR-2017
DEFINITION  Sequence verified.
ACCESSION
VERSION     .
KEYWORDS    Untitled.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Zheng and Zuo
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Mar 12, 2017 from SnapGene Viewer 3.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        26..44
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             127..417
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /note="SUMO"
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTP
                     LRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
     CDS             418..435
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     CDS             436..1221
                     /codon_start=1
                     /note="mouse ZFP57-full"
                     /translation="SSSCTGVFKGGPFFFCLTCGKCFKKNTFLFNHQFPVRSRRLAVT
                     NPQSRKGKGYKAQHRGERPFFCNFCGKTYRDASGLSRHRRAHLGYRPRSCPECGKCFR
                     DQSEVNRHLKVHQNKPAASNQAGNQASNQRLKSRVPPTTPRSQAPALKYVKVIQGPVA
                     RAKARNSGASTLNVRSNSITVVRSREKISCPYCHITFTMRTCLLTHLKIHFRRQPNQH
                     FCCKESAHSSNTLRMQKIYTCPVCDSSFRGKESLLDHLCCQRPI"
     misc_feature    481..546
                     /note="Finger 1"
     misc_feature    631..693
                     /note="Finger 2"
     misc_feature    715..777
                     /note="Finger 3"
     misc_feature    1003..1065
                     /note="Finger 4"
     misc_feature    1150..1221
                     /note="Finger 5"
     terminator      1376..1423
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1641..2229)
     CDS             complement(2401..3261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     promoter        complement(3262..3366)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN      
        1 gctagtggtg ctagccccgc gaaattaata cgactcacta tagggtctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg catcaccatc accatcacgg ttcttctatg
      121 gctagcatgt cggactcaga agtcaatcaa gaagctaagc cagaggtcaa gccagaagtc
      181 aagcctgaga ctcacatcaa tttaaaggtg tccgatggat cttcagagat cttcttcaag
      241 atcaaaaaga ccactccttt aagaaggctg atggaagcgt tcgctaaaag acagggtaag
      301 gaaatggact ccttaagatt cttgtacgac ggtattagaa ttcaagctga tcagacccct
      361 gaagatttgg acatggagga taacgatatt attgaggctc acagagaaca gattggtctg
      421 gtgccacgcg gtagttcctc cagttgcaca ggggtattca aaggtggacc attctttttc
      481 tgtctgacct gtggcaaatg tttcaaaaag aacaccttcc tctttaatca ccagtttcct
      541 gtgaggtccc ggaggctggc agtcacaaat ccacaaagcc gcaaaggcaa gggctacaag
      601 gctcagcatc gtggagagag gcctttcttt tgtaatttct gtggcaagac ttaccgtgat
      661 gcttctggac tgagccgtca ccgacgtgct catttaggtt ataggccccg ttcatgccct
      721 gagtgtggaa agtgtttccg ggatcagtct gaggtcaacc gtcacctgaa ggtacaccaa
      781 aacaagccag cagctagcaa ccaggctggc aaccaggcta gcaaccagag gctgaagagt
      841 agggttccac ctacaacacc tagatcccaa gcgcccgccc tcaagtatgt gaaagtgatc
      901 cagggaccag tggccagggc taaggcacgg aacagcggag cctcgaccct gaatgtcaga
      961 tccaactcta ttacagtggt ccgttcaaga gaaaagatct cttgtcccta ttgtcacata
     1021 acgtttacca tgagaacctg tctcttaacc cacctcaaga tccacttcag acgtcaaccc
     1081 aaccagcact tctgctgcaa agagtcggcc cactcatcca acacactcag aatgcaaaag
     1141 atctacactt gccccgtctg tgacagctcc tttaggggaa aggagagcct gctggatcac
     1201 ttgtgctgcc aaagaccaat ctaatgagga tcccgggaat tctcgagtaa ggttaacctg
     1261 caggaggcct ttaattaagg tggtgcggcc gcgctagcgg tcccggggga tcgatccggc
     1321 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     1381 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     1441 atccggaagc ttggcactgg ccgaccgggg tcgagcactg actcgctgcg ctcggtcgtt
     1501 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1561 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1621 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1681 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1741 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1801 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1861 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cgctaagaca cgacttatcg
     1981 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2041 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2101 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2161 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2221 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2281 tcacagatcc gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2341 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2401 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2461 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2521 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2581 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2641 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2701 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2761 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2821 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2881 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2941 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3001 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3061 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3121 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3181 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3241 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3301 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3361 tccgcgcaca tttccccgaa aagt
//
