LOCUS       Exported                4840 bp ds-DNA     circular SYN 19-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    1_pANCH2 = pFG4 corrected NatR#127
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 19, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     178..195
                     /note="SP6"
     misc_feature    282..297
                     /note="ParSc3"
     misc_feature    1007..1022
                     /note="ParSc3"
     misc_feature    1175..1190
                     /note="ParSc3"
     misc_feature    1325..1340
                     /note="ParSc3"
     misc_feature    1789..2361
                     /note="NatR"
     primer_bind     complement(2671..2690)
                     /note="T7"
     rep_origin      complement(2930..3558)
                     /direction=LEFT
                     /note="ColE1 origin"
     CDS             complement(3710..4369)
                     /note="AmpR"
     promoter        complement(4609..4637)
                     /note="Amp prom"
ORIGIN
        1 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
       61 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      121 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      181 taggtgacac tatagaacgc ggccgccagc tgaagcttcc ggcgtcaact tctatctact
      241 ccgaatccca ccattccatg aatcgcgcga ccgacgtgtg cgttgtcacg tgacaactgg
      301 cgctaaatat cggtgcgccc cgtactcgat tggaggctca agggaattca atggtagagc
      361 accgatcctc ccggctcatc ggcggatccc gcgaagaatc agccgaaacg tatcaaaaac
      421 aacaaatcac atatcaatca aaacaaatct agccgcagcc gtatcaatct tcaatgcccg
      481 ggcgttgaaa actcatacgc ccctatttcc cgagggtgca gcatccgttt tgcgtgaaac
      541 agcatgaaga atatggcgca aaccattgcc cgacaaggat ttccgggcga gtgcttattt
      601 tcgggaaatg tttcacgatc cgacccgttt gggttgttac atgcctctga cgtatggatt
      661 cagcgccagt agcagttcat acctgcggct atctccggca tcaacagtcc accagcgcag
      721 gcaccggctg gcggccacga agcaacctac cctatcggcc ggcacgcgtg gaaacgcagg
      781 gtcgcgctcc tataaatggg gacatatcat gacagcccca tgcagccttt gcgacagagc
      841 tcggcagcat tgcgtccgcc cttgagggtc cgcacgccgg caggaatttg gccgcgcaac
      901 cgatcgtgcc aaggccttcg cagccccaag ccgtttcttt gcgacagcaa cggggcgctc
      961 ctcgccatag agtcgccatc gcttgaactt cccggcatcg cgtcctgttg tcacgtgaca
     1021 actcaccgat ggacacacgg acaaaccacc tcggcgaaaa ctatcaactt cgtgcgtttc
     1081 cccgacaata tgcctccacg ccgcgagcgt cgaccagcaa caactattct taactgacca
     1141 caaatcccct atccgagaca gcaccttggg attgtttgtc acgtgacaac tcatcttccc
     1201 aggtagacgt ccgcgattta gcgcccgtgt atcgataatt agagtgaagc gattaccccg
     1261 cgctccaggt cgcaatcgaa aactttcgct cgacgtagca ctcggaggaa tcgatatccg
     1321 gcgacttgtc acgtgacaac cggctcttcc tcgttaccgg ggtctcagcg ccccggattt
     1381 gggttaatta aggcgcgcca gatctgttta gcttgccttg tccccgccgg gtcacccggc
     1441 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
     1501 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
     1561 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
     1621 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
     1681 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
     1741 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaccact
     1801 cttgacgaca cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag
     1861 gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac cggggacggc
     1921 ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa
     1981 tcggacgacg aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg
     2041 tacggggacg acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc
     2101 cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc
     2161 gcgttgatgg ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg
     2221 gaggtcacca acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc
     2281 tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac
     2341 atgagcatgc cctgccccta atcagtactg acaataaaaa gattcttgtt ttcaagaact
     2401 tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga
     2461 tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag
     2521 taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact
     2581 aacgccgcca tccagtgtcg aaaacgagct cgaattcatc gatgatatca gatccactag
     2641 tggcctatgc ggccgcggat ctgccggtct ccctatagtg agtcgtatta atttcgataa
     2701 gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2761 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2821 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3121 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     3181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3361 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3721 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3781 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3841 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3901 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3961 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4021 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4081 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4141 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4201 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4261 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4321 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4381 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4441 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4501 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4561 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4621 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4681 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4741 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4801 gacacatgca gctcccggag acggtcacag cttgtctgta
//
