LOCUS       Exported                4708 bp ds-DNA     circular SYN 19-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    4_pFG2.txt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 19, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     178..195
                     /note="SP6"
     misc_feature    224..1268
                     /note="ParSc2+"
                     /note="ParSc2+ = ParSc2 4 fois ParSc2 GTTTATGCGCATAAAC"
     misc_feature    1278..1656
                     /note="TEF-PRO"
     misc_feature    1657..2229
                     /note="NAT"
     misc_feature    2230..2469
                     /note="TEF-TER"
     primer_bind     complement(2539..2558)
                     /note="T7"
     rep_origin      complement(2798..3426)
                     /direction=LEFT
                     /note="ColE1 origin"
     CDS             complement(3578..4237)
                     /note="AmpR"
     promoter        complement(4477..4505)
                     /note="Amp prom"
ORIGIN
        1 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
       61 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      121 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      181 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
      241 ccccccattc ggacgatcgg tttatgcgca taaacgagcg gtggatgttc gatggctcgt
      301 ttggacggtg tggtgaagag gaagcggctg atgccgagtg atcgatgccc tgctcaggat
      361 tacgtcttca tgggcgtatc gtcggggccg ttcgtggcag agatatctcg acgctctctc
      421 ctttcgcggg acgagccgcg ttgcctgcga gccgcgagtt tgtgcgcata aagactgcgg
      481 aggccgatcg atgggcttcg tcgctagagc gcatggtatt cactcgcgag cgtccggcgt
      541 gagctgcgtg tcgtagtcag gtgcgtagat cacgaggtgc gtcgttgaat ggacgtgcat
      601 ggaggcaatg tcctgccggg ccgtagtgtt tatgcgcata aaccagaggc aacgataggc
      661 cgcgacgaag cgccgttgac agagactacg cctgagtcag gcgggcaatg acgtcatgtc
      721 ggggacgacg gtctatcgat atagccatcg cgcagtgccg ttgcgtgtcg acatatgtcc
      781 atgccacgag ccacgctgta cctcgcccgg caatcgcgcg ctttatgcgc ataaacctag
      841 ccggcgtgtt ccgcaatcgc ataccccttt cgcgacccga gacagccctc acgagacagc
      901 cctcacgaga cagccctcac gagacagccc tcacgagaca tcggctgcgc cggtcgtcgg
      961 ccccgcgaag ccacccgccc gactttatgc gcataaacaa ccactcttcg acgcgtcgcc
     1021 gaacacagga cactcccctc tcgcgatcac tgcgcatcgc caaaccgaaa ccgcctcagc
     1081 gacctatcaa gaggaacccc acaccgatct cgattgcccc ccatcaggcc cctcgatgca
     1141 gactcgctac aatacaggcc tcacccctcc tgccttgaac gatgatcacg atctaccaca
     1201 acccccgatg ctcgaaatcc cgcgagacgc ttgcgttggt cgagtcgctg aataccgccg
     1261 gcgcgccaga tctgtttagc ttgccttgtc cccgccgggt cacccggcca gcgacatgga
     1321 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
     1381 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
     1441 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
     1501 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
     1561 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
     1621 atacagttct cacatcacat ccgaacataa acaaccatgg gtaccactct tgacgacacg
     1681 gcttaccggt accgcaccag tgtcccgggg gacgccgagg ccatcgaggc actggatggg
     1741 tccttcacca ccgacaccgt cttccgcgtc accgccaccg gggacggctt caccctgcgg
     1801 gaggtgccgg tggacccgcc cctgaccaag gtgttccccg acgacgaatc ggacgacgaa
     1861 tcggacgacg gggaggacgg cgacccggac tcccggacgt tcgtcgcgta cggggacgac
     1921 ggcgacctgg cgggcttcgt ggtcgtctcg tactccggct ggaaccgccg gctgaccgtc
     1981 gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg tcgggcgcgc gttgatgggg
     2041 ctcgcgacgg agttcgcccg cgagcggggc gccgggcacc tctggctgga ggtcaccaac
     2101 gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt tcaccctctg cggcctggac
     2161 accgccctgt acgacggcac cgcctcggac ggcgagcagg cgctctacat gagcatgccc
     2221 tgcccctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
     2281 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
     2341 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
     2401 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc
     2461 cagtgtcgaa aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg
     2521 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc
     2581 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2641 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2701 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3001 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3241 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4561 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4621 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4681 tcccggagac ggtcacagct tgtctgta
//
