LOCUS Exported 6892 bp ds-DNA circular SYN 20-JAN-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pEF1a-DIO-FLPo-WPRE-hGHpA SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 6892) AUTHORS Huang Junxiang TITLE Direct Submission JOURNAL Exported Friday, Jan 20, 2017 from SnapGene Viewer 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6892 /organism="recombinant plasmid" /mol_type="other DNA" promoter 243..1421 /note="EF-1-alpha promoter" intron 474..1412 /note="EF-1-alpha intron A" protein_bind 1452..1485 /note="lox2272" protein_bind 1536..1569 /note="loxP" CDS complement(1581..2879) /codon_start=1 /note="FLPo" /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI" protein_bind complement(2889..2922) /note="lox2272" protein_bind complement(2973..3006) /note="loxP" misc_feature 3031..3619 /note="WPRE" CDS complement(3502..3513) /codon_start=1 /note="Factor Xa site" /translation="IEGR" polyA_signal 3651..4127 /note="hGH poly(A) signal" /note="human growth hormone polyadenylation signal" repeat_region 4167..4307 /note="AAV2 ITR" rep_origin 4382..4837 /direction=RIGHT /note="f1 ori" promoter 5119..5223 /note="AmpR promoter" CDS 5224..6084 /codon_start=1 /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6255..6843 /direction=RIGHT /note="ori" ORIGIN 1 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt 61 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 121 aactccatca ctaggggttc ctgcggccgc acgcgtaagc tttgcaaaga tggataaagt 181 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa 241 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 301 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 361 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 421 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 481 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 541 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 601 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 661 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 721 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 781 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 841 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 901 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 961 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 1021 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 1081 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 1141 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 1201 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 1261 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 1321 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 1381 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggtaccgga tcctctagag 1441 tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga atggtagctg 1501 gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc atacattata 1561 cgaagttatg gcgcgcctta tcagatccgc ctgttgatgt agctgctcag gtagtccagc 1621 acctcctggc tgatgatgcc gttccaggcg gggtatctga tgctgccctc ggcgctgccc 1681 ttcagctgct cgatgtgctg ccactcctcg atggggttgg tctcgtcctt cagggcgatc 1741 atctccttgc tgatggggtc gtaggcgtag tacctggaca ccagggcgaa gtagtggtcg 1801 gggatggcgg tgatctggtg ggtgtaggtg gtcctggcca cggcggaggc cctcttgtcg 1861 ctccagttgc ccaccacgtt tgtcagctcg gtcaggccct tcatgctcag aaagctggtc 1921 atcaggtgcc tgccgatgtg gctcttaggg ccgttcttga tagcgaagat ggggtagggg 1981 gcgttcttct tcagggcctt gttgtagctg cgcaccaggt tgtccttcag cagctggtac 2041 tcctgcttgt tgctgctgct gttgccggtc ctgttcactc tcttcagcac gggctcgctg 2101 ttcctcagga actcgtccag gtacaccagg gggtcgatcc tgcctctggc gctgaaaaag 2161 tagatgtgcc tggacacgct tgtcttggtc tcggtcacca ggcactgaat gatcacgccc 2221 aggtacttgt tctgcaccag cttgaagctc ttggggtcca cgttcttgat gtcgctgaac 2281 ctgccgcagt tgatgaatgt ggccaggaac aggaactggt acagggtctt ggtcttggtg 2341 aacctgctgg tgtactcgaa gctgttcagg atcttctcgg tgatctccca gatgctctcg 2401 ccctcggaca gcagggcctt cagcatcttc ttgctgtggc tgttgccctt gtcggcctcc 2461 tcgctgctct cgaactgcag ctgcaggctg gacacgatgt cggtgatgtc gctctggtgc 2521 ttctggccgt tgtaagggat gatggtgaac tcccaggcgg ggatcagctt cttcaggctg 2581 gcctccagga tggtggcctt ctgggtcttg tacttgaact gcaggctctt gttcacgatg 2641 tcgaagctca ggctgttgct gatgatggtg ttgtagctca tgaaggtggc cctcttgatg 2701 gcggtgccgt tgtgggtgat catccagcac aggtaggtca gctcggcggc acagctggcg 2761 atcttctcgc cgctgggcct ctcgaatctc tccacgaact gccgcaccag caccttgggg 2821 ggggtcttgc acaggatgtc gaactggctc atcaccttcc tcttcttctt aggagccatg 2881 gtgctagcat aacttcgtat aaagtatcct atacgaagtt atttgcctta acccagaaat 2941 tatcactgtt attctttaga atggtgcaaa gaataacttc gtataatgta tgctatacga 3001 agttatgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa 3061 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 3121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 3181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 3241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 3301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 3361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 3421 gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg 3481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 3541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 3601 ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc 3661 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca 3721 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata 3781 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg 3841 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa 3901 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc 3961 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca 4021 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca 4081 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg 4141 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc 4201 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 4261 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg 4321 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag 4381 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 4441 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 4501 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 4561 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg 4621 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 4681 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta 4741 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt 4801 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc 4861 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc 4921 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc 4981 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg 5041 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc 5101 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 5161 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 5221 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 5281 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 5341 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 5401 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 5461 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 5521 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 5581 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 5641 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 5701 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 5761 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 5821 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 5881 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 5941 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 6001 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 6061 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 6121 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 6181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 6241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 6301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 6361 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 6421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 6481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc tt //