LOCUS       FJ687158                2864 bp    DNA     linear   SYN 11-MAR-2009
DEFINITION  RNAi Expression Vector pUC18-H1, complete sequence.
ACCESSION   FJ687158
VERSION     FJ687158.1
KEYWORDS    .
SOURCE      RNAi Expression Vector pUC18-H1
  ORGANISM  RNAi Expression Vector pUC18-H1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Mathupala,S.P., Parajuli,P. and Sloan,A.E.
  TITLE     Silencing of monocarboxylate transporters via small interfering
            ribonucleic acid inhibits glycolysis and induces cell death in
            malignant glioma: an in vitro study
  JOURNAL   Neurosurgery 55 (6), 1410-1419 (2004)
   PUBMED   15574223
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Mathupala,S.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2009) Neurological Surgery, Wayne State
            University School of Medicine, 4100 John R. Road, 607 HWCRC,
            Detroit, MI 48201, USA
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="RNAi Expression Vector pUC18-H1"
                     /mol_type="other DNA"
                     /db_xref="taxon:617124"
                     /note="derived from glioma cell line U87-MG (ATCC HTB-14);
                     shRNA expression vector containing U87MG glioma H1 RNA
                     promoter"
     gene            complement(<239..>307)
                     /gene="lacZ"
     CDS             complement(<239..>307)
                     /gene="lacZ"
                     /codon_start=1
                     /transl_table=11
                     /product="lacZ alpha peptide"
                     /protein_id="ACN59900.1"
                     /translation="WRNSEEARTDRPSQQLRSLNGEW"
     misc_feature    378..395
                     /note="M13 forward primer site"
     misc_feature    399..413
                     /note="multiple cloning site (HinDIII-XbaI-BglII)"
     regulatory      complement(414..633)
                     /regulatory_class="promoter"
                     /note="H1 RNA promoter (-230 to -9) cloned from glioma
                     cell line U87-MG"
     misc_feature    complement(639..659)
                     /note="M13 reverse primer site"
     misc_feature    complement(665..687)
                     /note="lacO (lac operon)"
     rep_origin      complement(1027..1813)
                     /note="Col E1 replication origin (modified pMB1
                     replication origin from plasmid vector pBR322)"
     gene            complement(1804..2466)
                     /gene="bla"
     CDS             complement(1804..2466)
                     /gene="bla"
                     /note="confers resistance to ampicillin (Ap-R)"
                     /codon_start=1
                     /transl_table=11
                     /product="beta lactamase"
                     /protein_id="ACN59901.1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                     LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
                     EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
                     WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
                     HW"
ORIGIN      
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttctaga tctgtggtct
      421 catacagaac ttataagatt cccaaatcca aagacatttc acgtttatgg tgatttccca
      481 gaacacatag cgacatgcaa atattgcagg gcgccactcc cctgtccctc acagccatct
      541 tcctgccagg gcgcacgcgc gctgggtgtt cccgcctatg acactgggcc cgcgattcct
      601 tggagcgggt tgatgacgtc agcgttcgaa ttcgtaatca tggtcatagc tgtttcctgt
      661 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      721 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      781 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      841 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1021 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     1501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1621 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1681 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1741 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1801 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1861 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1921 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1981 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2041 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2101 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2161 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2221 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2281 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2341 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2401 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2461 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2521 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2581 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2641 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2701 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2761 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2821 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//

