LOCUS       pCG-D-SpyTag-E          1746 bp ds-DNA     circular     20-FEB-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        complement(1272..1376)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(503..611)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1377..394)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    414..452
                     /label="SpyTag Bs opt 2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    459..476
                     /label="his6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(612..1271)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
       61 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      121 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      181 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      241 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      301 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      361 tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg GGTCTCaGGC AgcGCCCACA
      421 TCGTTATGGT AGACGCGTAC AAACCTACAA AGGGAAGCCA TCATCATCAT CATCACGGCA
      481 GCTAAGTTCt GAGACCgacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
      541 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
      601 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
      661 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
      721 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
      781 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
      841 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
      901 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
      961 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1021 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     1081 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     1141 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     1201 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     1261 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     1321 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     1381 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1441 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1501 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1561 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1621 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1681 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1741 gttacc
//