LOCUS Exported 11045 bp ds-DNA circular SYN 05-APR-2017 DEFINITION synthetic circular DNA ACCESSION pcut-R_607c VERSION . KEYWORDS 87409.JBEI-14604 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11045) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Apr 5, 2017 from SnapGene 3.3.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11045 /organism="synthetic DNA construct" /mol_type="other DNA" source 3919..4623 /organism="synthetic DNA construct" /mol_type="other DNA" /note="synthetic DNA construct" source 3919..4623 /organism="synthetic DNA construct" /mol_type="other DNA" /note="synthetic DNA construct" source 4625..8784 /organism="synthetic DNA construct" /mol_type="other DNA" /note="synthetic DNA construct" rep_origin 69..1411 /note="2u ori" /note="yeast 2u plasmid origin of replication 2u ori" protein_bind 401..448 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011). FRT" promoter 1438..1542 /gene="bla" /note="bla" /note="AmpR promoter" CDS 1543..2403 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="bla" /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2574..3162 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication ori" promoter 3486..3516 /note="lac promoter" /note="promoter for the E. coli lac operon lac promoter" protein_bind 3524..3540 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). lac operator" primer_bind 3548..3564 /note="M13 rev" /note="M13 rev common sequencing primer, one of multiple similar variants" misc_feature 3565..3584 /note="cut607c" promoter 3585..3603 /note="T3 promoter" /note="T3 promoter promoter for bacteriophage T3 RNA polymerase" promoter 3621..3737 /note="pTyrosine(1)" /note="Tyrosine tRNA promoter pTyrosine" misc_RNA 3758..3833 /note="gRNA scaffold" /note="guide RNA scaffold for the CRISPR/Cas9 system gRNA scaffold" misc_feature 3838..3912 /note="snr52T" /note="SNR52 terminator" promoter 3919..4623 /gene="S. cerevisiae ADH1" /note="S. cerevisiae ADH1" /note="promoter for alcohol dehydrogenase 1 ADH1 promoter" CDS 4633..8772 /codon_start=1 /product="Streptococcus pyogenes Cas9 Gene (codon optimized for expression in human cells)" /note="Cas9_hCO" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGDSRADPKKKRKV" CDS 7220..7231 /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" CDS 8749..8769 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" terminator 8785..9032 /gene="S. cerevisiae CYC1" /note="S. cerevisiae CYC1" /note="CYC1 terminator transcription terminator for CYC1" promoter complement(9051..9069) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase T7 promoter" rep_origin 9240..9695 /direction=RIGHT /note="f1 ori" /note="f1 ori f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(9826..10629) /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /note="S. cerevisiae URA3" /note="URA3 yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(10630..10845) /gene="S. cerevisiae URA3" /note="S. cerevisiae URA3" /note="URA3 promoter" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg 121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg 181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac 241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata 301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc 361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt 421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa 481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga 541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat 601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct 661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta 721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag 781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc 841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct 901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt 961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg 1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa 1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa 1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta 1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt 1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt 1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt 1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc 1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc 3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa 3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 3601 agggaacaaa agctggagct agtatactct ttcttcaaca attaaatact ctcggtagcc 3661 aagttggttt aaggcgcaag actgtaattt atcactacga aatcttgaga tcgggcgttc 3721 gactcgcccc cgggagacta tttttgcttt ctgcacagtt ttagagctag aaatagcaag 3781 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt 3841 attttttgtc actattgtta tgtaaaatgc cacctctgac agtatggaac gcaaacttct 3901 gtctagtgga tagtcgacat ccttttgttg tttccgggtg tacaatatgg acttcctctt 3961 ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca 4021 tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta 4081 aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt 4141 agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca 4201 tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt ttctctctcc 4261 cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa 4321 aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc 4381 tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac 4441 ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc 4501 tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt 4561 ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca 4621 acttggaaaa aaatggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc 4681 ggttgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc 4741 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg 4801 gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag 4861 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct 4921 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac 4981 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat 5041 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc 5101 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca 5161 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc 5221 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg 5281 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc 5341 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc 5401 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac 5461 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg 5521 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg 5581 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag 5641 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa 5701 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt 5761 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa 5821 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg 5881 ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac 5941 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc 6001 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg 6061 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact 6121 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag 6181 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag 6241 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac 6301 cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac 6361 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat 6421 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt 6481 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg 6541 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat 6601 acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga 6661 aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt catgcagttg 6721 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag 6781 ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga 6841 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc 6901 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac 6961 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt 7021 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg 7081 cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac 7141 gacgtggatc atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg 7201 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc 7261 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag 7321 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc 7381 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat 7441 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt 7501 actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga 7561 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca 7621 cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac 7681 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac 7741 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag 7801 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag 7861 ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa 7921 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc 7981 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct 8041 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa 8101 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa 8161 aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt 8221 aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt 8281 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg 8341 tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag 8401 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc 8461 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag 8521 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc 8581 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac 8641 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat 8701 gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag 8761 aggaaggtgt gatctcttct cgagtcatgt aattagttat gtcacgctta cgttcacgcc 8821 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc 8881 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc 8941 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga 9001 aggttttggg acgctcgaag gctttaattt gcggccggta cccaattcgc cctatagtga 9061 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 9121 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 9181 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga 9241 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 9301 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 9361 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 9421 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 9481 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 9541 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 9601 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 9661 cgcgaatttt aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg 9721 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct 9781 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc 9841 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc 9901 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac 9961 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac 10021 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc 10081 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata 10141 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg 10201 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac 10261 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg 10321 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta 10381 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat 10441 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa 10501 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat 10561 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc 10621 tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa 10681 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct 10741 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaagg 10801 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa aagctgtggt 10861 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc 10921 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 10981 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 11041 cgcga //