LOCUS       87421_AICSDP-4:T        2731 bp    DNA     linear   UNA 12-APR-2018
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local.amandah.7i-8vznsaj
VERSION     urn.local.amandah.7i-8vznsaj
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     gene            <1..>1000
                     /gene="TUBA1B"
                     /gene_synonym="K-ALPHA-1"
                     /note="tubulin, alpha 1b; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq."
                     /db_xref="GeneID:10376"
                     /db_xref="HGNC:HGNC:18809"
                     /db_xref="MIM:602530"
                     /label="TUBA1B gene"
     source          <1..>1000
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /chromosome=12
                     /label="source Homo sapiens"
     modified_base   888
                     /created_by="User"
                     /label="C to A"
     misc_RNA        complement(891..910)
                     /created_by="User"
                     /label="crRNA 2 binding"
     modified_base   990
                     /created_by="User"
                     /label="C to A"
     misc_RNA        complement(993..>1000)
                     /created_by="User"
                     /label="crRNA 1 binding"
     modified_base   994
                     /created_by="User"
                     /label="C to A"
     misc_RNA        complement(997..>1000)
                     /created_by="User"
                     /label="crRNA 3 binding"
     ORF             1001..1714
                     /created_by="User"
                     /modified_by="User"
                     /label="mEGFP"
     misc_feature    1715..1732
                     /created_by="User"
                     /modified_by="User"
                     /label="GGSGGS linker"
     CDS             join(<1733..1952,2062..2210,2514..>2731)
                     /gene="TUBA1B"
                     /gene_synonym="K-ALPHA-1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /codon_start=1
                     /product="tubulin alpha-1B chain"
                     /protein_id="NP_006073.2"
                     /db_xref="GI:57013276"
                     /db_xref="CCDS:CCDS31792.1"
                     /db_xref="GeneID:10376"
                     /db_xref="HGNC:HGNC:18809"
                     /db_xref="MIM:602530"
                     /translation="MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGG
                     GDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANN
                     YARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVD
                     YGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLD
                     IERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVI
                     SAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAT
                     IKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLD
                     HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEE
                     Y"
                     /label="TUBA1B CDS"
     mRNA            join(<1733..1952,2062..2210,2514..>2731)
                     /gene="TUBA1B"
                     /gene_synonym="K-ALPHA-1"
                     /product="tubulin, alpha 1b"
                     /note="Derived by automated computational analysis using
                     gene prediction method: BestRefSeq."
                     /transcript_id="NM_006082.2"
                     /db_xref="GI:57013275"
                     /db_xref="GeneID:10376"
                     /db_xref="HGNC:HGNC:18809"
                     /db_xref="MIM:602530"
                     /label="TUBA1B mRNA"
     gene            <1733..>2731
                     /gene="TUBA1B"
                     /gene_synonym="K-ALPHA-1"
                     /note="tubulin, alpha 1b; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq."
                     /db_xref="GeneID:10376"
                     /db_xref="HGNC:HGNC:18809"
                     /db_xref="MIM:602530"
                     /label="TUBA1B gene"
     source          <1733..>2731
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
                     /chromosome=12
                     /label="source Homo sapiens"
     misc_RNA        complement(<1733..1745)
                     /created_by="User"
                     /label="crRNA 3 binding"
     misc_RNA        complement(<1733..1741)
                     /created_by="User"
                     /label="crRNA 1 binding"
ORIGIN      
        1 aaagcaataa gaggactgcg gaagagctcc ctgtcaatgt accgctctac accagtgtat
       61 tacgacagtt cgtacacaac agtctgtaga ggccacctgt ctctccctgc tgcgttagga
      121 attcagggga gcaggtggtg gcagtaaggg attttgaggg aacggaaatc ggatcttgac
      181 ccagatctgg gccgccgata atctcctact gcgctcagac tgctgtggag gtgttaggct
      241 gagcccgatg ccggcaggca agggaggatg ggcggcttgg gcagcgcctt tgcagacgtg
      301 gccatttcgt gcctctgcag caccgccggg gggcgcaaga gcgcgcgccc ggaattgctc
      361 attcatcctg tgccgcagag ccccgcccct tgtccctgcg gacagacatt tcttctgcgc
      421 tggtctggcc acgtgcttcc tgtgctagga gctgcccgga aatgtgacca cctagtctaa
      481 agtgggcttc tggggcctga gcgctggatg gatgcccacc ttcctgtctt ggtcctccaa
      541 aggaggaagc tgtgactgag ctgtcttggt ctggaaggag gccttcccgg tttaggatgg
      601 gaaggtaaca ttcattaaaa gcaacgtaga ctatagtgta gctgttctca aaagtagtac
      661 atcttagaaa aggatcttta gaaaagatcg ctttagaaaa ggaaattcgt tttcagatta
      721 cgtgagtagc ctaggtaaca cagccagacc tcatctccac aaaaaaaatg aaaaaattag
      781 ccagcttggt ggtctgtgcc tgtggtccca gctgctccag aggctgaggt ggggggatga
      841 ctggagccta ggctgcagtg agcctagatg gcatcactgc actcaagact gggcgacaga
      901 ccttatctct aaaaaaataa agattgcatg agtattttgt tccacttgac agtcatcaat
      961 agattggttt aaattgtgat atctttttta cttaccgcag gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagggaggt tcaggaggca gcgagtgcat
     1741 ctccatccac gttggccagg ctggtgtcca gattggcaat gcctgctggg agctctactg
     1801 cctggaacac ggcatccagc ccgatggcca gatgccaagt gacaagacca ttgggggagg
     1861 agatgactcc ttcaacacct tcttcagtga gacgggcgct ggcaagcacg tgccccgggc
     1921 tgtgtttgta gacttggaac ccacagtcat tggtgagttg acctcagtaa cctgagatcc
     1981 caggatgctg ggacaggagg tctgtccagg ggcttctctt gtcactcact cactccctcc
     2041 gtccttctct ccctcctcca gatgaagttc gcactggcac ctaccgccag ctcttccacc
     2101 ctgagcagct catcacaggc aaggaagatg ctgccaataa ctatgcccga gggcactaca
     2161 ccattggcaa ggagatcatt gaccttgtgt tggaccgaat tcgcaagctg gtaagcacca
     2221 catataaata tgcatttaat gtggtgtgat agttccagtg caagttgggt ggagtgactg
     2281 acatcattca ttctttggca cctaccaaaa tgtggaatag gctgcttgct atattaattg
     2341 gacttctaaa tcagatagtc cctaggttat ggacagtttg tggatatgtc tgttttgcca
     2401 attccttgtg cttacatcag tgagatatgg ttcgtaatct aaaaagttga aatagaaatt
     2461 ctaagataat gtgtcctggc attaaaatat tacatttttt tattccccta caggctgacc
     2521 agtgcaccgg tcttcagggc ttcttggttt tccacagctt tggtggggga actggttctg
     2581 ggttcacctc cctgctcatg gaacgtctct cagttgatta tggcaagaag tccaagctgg
     2641 agttctccat ttacccagca ccccaggttt ccacagctgt agttgagccc tacaactcca
     2701 tcctcaccac ccacaccacc ctggagcact c
//
