LOCUS       2mU6_XX                 3101 bp ds-DNA     circular     27-APR-2015
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2057..2914
                     /label=Amp(R)
                     /ApEinfo_fwdcolor=#9c6378
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    30..988
                     /vntifkey="21"
                     /label=U6\promoter
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#b89aa6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    989..991
                     /label=EcoRV remnant
                     /ApEinfo_fwdcolor=#808080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1015..1015
                     /label=BsaI mutated
                     /ApEinfo_fwdcolor=#c3063c
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2198..2198
                     /label=BsaI mutated(1)
                     /ApEinfo_label=BsaI mutated
                     /ApEinfo_fwdcolor=#c3063c
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1042..1061
                     /label=backbone seq rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1004..1009
                     /label=BglII
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=#800080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aggcccttac gtattaatta aggcgcgcct ggcgggtgta ttaaaccact aaacaaacaa
       61 ttgccccaag ctctcttcac aattataaac actataatgt ttggacaaga gattagcgtg
      121 gctgtgacga gaactctcaa aggcttggtg taattgatat tttataagaa gcagattaaa
      181 cttcaataca gtttacacct catttacaaa aaattggctg ccaaaatcgc taatttacac
      241 atatttaaaa caatttcaac acatatacac agtatagtat aactgcataa taaacaaata
      301 catctaacag acactcacta atctgccata acaagcttca aaaacttaaa ctcgaaattt
      361 tagtgaatct ttttttttaa atgaatattt tatttaaaaa gttaaaaata ttacagttca
      421 ggtataggtt tacacctaat ctttaataat ccgaactaaa ttttaactat ttagaaactt
      481 tttcaaccaa agtttaaaaa aatagatttt tcgcacgcta aaactatcat ttacacaaaa
      541 aaatgcaaca aaatgcagaa aaaaattaca ttagagttta ggttagttac ctgctaatca
      601 atataaacta acttcccgca taatattcat ttaaaattag caataatcac gttttacgct
      661 aaaatttgtg taaaactaaa cttcgtcctt tgtcaaggag aaaatttgac tcaaaagctg
      721 cgcgcgcagg ggagatcccc aagcgagtgt ttgttacatc ataatcatgt ggaaaaatcc
      781 cctaataagt aaaaatatat ttttttttaa tttttggggc gaataaaccg ctttttatgt
      841 ctaaaaacgc caaaaatgga tcgcgcgagc ccaaaaacgc acaaataacg tacagacagt
      901 gtctctgcgt acacagacgg tatttcccct ttaaattgag aactagactt aagcacgctt
      961 ataagtctgg aaggcatccg atggtataga tGCAGTGGAG GCAagatctg ccggActccc
     1021 tatagtgagt cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac
     1081 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1141 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1201 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1261 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1321 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1381 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1441 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     1501 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1561 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1621 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1681 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1741 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1801 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2041 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2101 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2161 taccatctgg ccccagtgct gcaatgatac cgcgagaTcc acgctcaccg gctccagatt
     2221 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2281 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2341 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2401 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2461 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2521 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2581 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2641 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2701 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2761 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2821 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2881 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2941 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3001 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     3061 ttattatcat gacattaacc tataaaaata ggcgtatcac g 
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