LOCUS       Exported                7707 bp ds-DNA     circular SYN 09-MAY-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1-STIM1-Venus173-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 9, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
COMMENT     [DEFINITION]:  - [LOCUS]: pcDNA\3.1\[STIM1 - [CDS]: 2997..30
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..617
                     /note="CMV Promoter"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&hF08080
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #f08080; direction: RIGHT"
     primer_bind     628..647
                     /note="T7"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h008B8B
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     628..646
                     /note="T7 Promoter"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h84A4C0
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             701..2755
                     /note="STIM1"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h84A4C0
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             2762..2791
                     /note="Linker"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h84A4C0
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #84a4c0; direction: RIGHT"
     CDS             2792..2992
                     /note="Venus173-C"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&hFFFF00
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(3066..3083)
                     /note="BGH-rev"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h84A4C0
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #84a4c0; direction: LEFT"
     terminator      3069..3296
                     /note="bGH PA"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&hFF8000
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #ff8000; direction: RIGHT"
     misc_feature    3964..4041
                     /note="SV40 ORI"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h4682B4
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #4682b4; direction: RIGHT"
     CDS             4183..4974
                     /note="Kan/neoR"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h8FBC8F
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #8fbc8f; direction: RIGHT"
     misc_feature    4183..4971
                     /note="KanR"
                     /note="vntifkey=""21"" - label=KanR - 4418..5206
                     SerialCloner_Protect=True
                     SerialCloner_Color=&hDC143C
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True
                     SerialCloner_Desc=vntifkey=""21"" - label=KanR - 
                     4418..5206"
                     /note="color: #dc143c; direction: RIGHT"
     primer_bind     complement(5314..5334)
                     /note="M13-rev"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h008B8B
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #008b8b; direction: LEFT"
     misc_feature    complement(5314..5332)
                     /note="M13rev"
                     /note="vntifkey=""21"" - label=M13rev - 
                     complement(5549..5567)
                     SerialCloner_Protect=True
                     SerialCloner_Color=&hDC143C
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True
                     SerialCloner_Desc=vntifkey=""21"" - label=M13rev - 
                     complement(5549..5567)"
                     /note="color: #dc143c; direction: LEFT"
     misc_binding    complement(5340..5362)
                     /note="LacO"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h6495ED
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #6495ed; direction: LEFT"
     promoter        complement(5367..5396)
                     /note="lac"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h4682B4
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #4682b4; direction: LEFT"
     misc_feature    complement(6476..7336)
                     /note="AmpR"
                     /note="vntifkey=""21"" - label=AmpR - 
                     complement(6711..7571)
                     SerialCloner_Protect=True
                     SerialCloner_Color=&hDC143C
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True
                     SerialCloner_Desc=vntifkey=""21"" - label=AmpR - 
                     complement(6711..7571)"
                     /note="color: #dc143c; direction: LEFT"
     CDS             complement(6479..7138)
                     /note="AmpR"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h8FBC8F
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #8fbc8f; direction: LEFT"
     promoter        complement(7378..7406)
                     /note="Amp prom"
                     /note="SerialCloner_Protect=True
                     SerialCloner_Color=&h4682B4
                     SerialCloner_Show=True
                     SerialCloner_Arrow=True"
                     /note="color: #4682b4; direction: LEFT"
ORIGIN
        1 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      601 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
      661 ctagcgttta aacttaagct tggtaccgag ctcggatccc atggatgtat gcgtccgtct
      721 tgccctgtgg ctcctctggg gtctcctcct gcaccaaggc cagtcgctca gccatagtca
      781 cagtgagaag gcgacaggaa ccagctcggg ggccaactct gaggagtcca ctgcagcaga
      841 gttttgccga attgacaagc ccctgtgtca cagtgaggat gagaaactca gcttcgaggc
      901 agtccgtaac atccacaaac tgatggacga tgatgccaat ggtgatgtgg atgtggaaga
      961 aagtgatgag ttcctgaggg aagacctcaa ttaccatgac ccaacagtga aacacagcac
     1021 cttccatggt gaggataagc tcatcagcgt ggaggacctg tggaaggcat ggaagtcatc
     1081 agaagtatac aattggaccg tggatgaggt ggtacagtgg ctgatcacat atgtggagct
     1141 gcctcagtat gaggagacct tccggaagct gcagctcagt ggccatgcca tgccaaggct
     1201 ggctgtcacc aacaccacca tgacagggac tgtgctgaag atgacagacc ggagtcatcg
     1261 gcagaagctg cagctgaagg ctctggatac agtgctcttt gggcctcctc tcttgactcg
     1321 ccataatcac ctcaaggact tcatgctggt ggtgtctatc gttattggtg tgggcggctg
     1381 ctggtttgcc tatatccaga accgttactc caaggagcac atgaagaaga tgatgaagga
     1441 cttggagggg ttacaccgag ctgagcagag tctgcatgac cttcaggaaa ggctgcacaa
     1501 ggcccaggag gagcaccgca cagtggaggt ggagaaggtc catctggaaa agaagctgcg
     1561 cgatgagatc aaccttgcta agcaggaagc ccagcggctg aaggagctgc gggagggtac
     1621 tgagaatgag cggagccgcc aaaaatatgc tgaggaggag ttggagcagg ttcgggaggc
     1681 cttgaggaaa gcagagaagg agctagaatc tcacagctca tggtatgctc cagaggccct
     1741 tcagaagtgg ctgcagctga cacatgaggt ggaggtgcaa tattacaaca tcaagaagca
     1801 aaatgctgag aagcagctgc tggtggccaa ggagggggct gagaagataa aaaagaagag
     1861 aaacacactc tttggcacct tccacgtggc ccacagctct tccctggatg atgtagatca
     1921 taaaattcta acagctaagc aagcactgag cgaggcgaca gcagcattgc gggagcgcct
     1981 gcaccgctgg caacagatcg agatcctctg tggcttccag attgtcaaca accctggcat
     2041 ccactcactg gtggctgccc tcaacataga ccccagctgg atgggcagta cacgccccaa
     2101 ccctgctcac ttcatcatga ctgacgacgt ggatgacatg gatgaggaga ttgtgtctcc
     2161 cttgtccatg cagtccccta gcctgcagag cagtgttcgg cagcgcctga cggagccaca
     2221 gcatggcctg ggatctcaga gggatttgac ccattccgat tcggagtcct ccctccacat
     2281 gagtgaccgc cagcgtgtgg cccccaaacc tcctcagatg agccgtgctg cagacgaggc
     2341 tctcaatgcc atgacttcca atggcagcca ccggctgatc gagggggtcc acccagggtc
     2401 tctggtggag aaactgcctg acagccctgc cctggccaag aaggcattac tggcgctgaa
     2461 ccatgggctg gacaaggccc acagcctgat ggagctgagc ccctcagccc cacctggtgg
     2521 ctctccacat ttggattctt cccgttctca cagccccagc tccccagacc cagacacacc
     2581 atctccagtt ggggacagcc gagccctgca agccagccga aacacacgca ttccccacct
     2641 ggctggcaag aaggctgtgg ctgaggagga taatggctct attggcgagg aaacagactc
     2701 cagcccaggc cggaagaagt ttcctctcaa aatctttaag aagcctctta agaagggtac
     2761 cggaggtgga ggtagcggag gtggaggtag tgaggacggc ggcgtgcagc tcgccgacca
     2821 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     2881 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2941 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt
     3001 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg
     3061 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3121 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3181 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3241 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3301 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3361 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3421 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3481 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3541 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3601 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3661 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3721 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     3781 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3841 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3901 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3961 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4021 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4081 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4141 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4201 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4261 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4321 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4381 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4441 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4501 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4561 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4621 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4681 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4741 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4801 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4861 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4921 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4981 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5041 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5101 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5161 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5221 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5281 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5341 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5401 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5461 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5521 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5581 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5641 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5701 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5761 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5821 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5881 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5941 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6001 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6061 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6121 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6181 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6241 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6301 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6361 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6421 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6481 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6541 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6601 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6661 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6721 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6781 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6841 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6901 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6961 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7021 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7081 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7141 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7201 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7261 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7321 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7381 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7441 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     7501 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     7561 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     7621 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     7681 cgatgtacgg gccagatata cgcgttg
//
