LOCUS       pcDNA3-ER-rsTagRFP.xdna    6211 bp      DNA     circular   1/27/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(4050..4070)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(4438..5066)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(4076..4098)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(5218..5877)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             2919..3710
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(6117..6145)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(4103..4132)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2700..2777
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1007..1702
                     /label=rsTagRFP
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(1764..1781)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     sig_peptide     920..1000
                     /label=ER-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      1804..2031
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTCAGTTG 
      901 TCAGGAAGAG AAGGCTCCCA TGGACCCTGT GGTGGTGCTG GGGCTCTGTC TCTCCTGTTT 
      961 GCTTCTCCTT TCACTCTGGA AACAGAGCTA TGGGGGAGGG GATCCGATGA GCGAGCTGAT 
     1021 TAAGGAGAAC ATGCACATGA AGCTGTACAT GGAGGGCACC GTGAACAACC ACCACTTCAA 
     1081 GTGCACATCC GAGGGCGAAG GCAAGCCCTA CGAGGGCACC CAGACCATGA GAATCAAGGT 
     1141 GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT CGACATCCTG GCTACCAGCT TCATGTACGG 
     1201 CAGCCGCACC TTCATCAACC ACACCCAGGG CATCCCCGAC TTCTGGAAGC AGTCCTTCCC 
     1261 TGAGGGCTTC ACATGGGAGA GAGTCACCAC ATACGAAGAC GGGGGCGTGC TGACCGCTAC 
     1321 CCAGGACACC AGCCTCCAGG ACGGCTGCCT CATCTACAAC GTCAAGCTCA GAGGGGTGAA 
     1381 CTTCCCATCC AACGGCCCTG TGATGCAGAA GAAAACACTC GGCTGGGAGG CCGCCACCGA 
     1441 GATGCTGTAC CCCGCTGACG GCGGCCTGGA AGGCAGAGGG GACATGGCCC TGAAGCTCGT 
     1501 GGGCGGGGGC CACCTGATCT GCAACTTGAA GACCACATAC AGATCCAAGA ATCCCGCTAA 
     1561 GAACCTCAAG ATGCCCGGCG TCTACTTTGT GGACCACAGA CTGGAAAGAA TCAAGGAGGC 
     1621 CGACAAAGAG ACCTACGTCG AGCAGCACGA GGTGGCTGTG GCCAGATACT GCGACCTCCC 
     1681 TAGCAAACTG GGGCACAAGT AAGAATTCTG CAGATATCCA TCACACTGGC GGCCGCTCGA 
     1741 GCATGCATCT AGAGGGCCCT ATTCTATAGT GTCACCTAAA Tgctagagct cgctgatcag 
     1801 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
     1861 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
     1921 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
     1981 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 
     2041 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 
     2101 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     2161 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     2221 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     2281 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     2341 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     2401 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 
     2461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 
     2521 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca 
     2581 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     2641 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     2701 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     2761 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 
     2821 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 
     2881 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
     2941 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     3001 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     3061 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 
     3121 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     3181 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     3241 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     3301 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     3361 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     3421 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 
     3481 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     3541 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     3601 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     3661 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
     3721 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
     3781 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     3841 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 
     3901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     3961 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 
     4021 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     4081 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     4141 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     4201 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     4261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     4321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     4381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     4441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     4501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     4561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     4621 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 
     4681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     4741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     4801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     4861 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     4921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     4981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     5041 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     5101 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     5161 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     5221 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     5281 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     5341 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     5401 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     5461 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     5521 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     5581 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     5641 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     5701 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     5761 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     5821 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     5881 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     5941 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     6001 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     6061 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     6121 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     6181 cacatttccc cgaaaagtgc cacctgacgt c
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