LOCUS Exported 9669 bp ds-DNA circular SYN 16-NOV-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9669) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 16, 2017 from SnapGene 4.0.7 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9669 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 232..819 /label=CMV prom /note="color: #800080; direction: RIGHT" primer_bind 863..882 /label=T7 /note="color: #008b8b; direction: RIGHT" CDS 922..939 /label=6His /note="color: #008000; direction: RIGHT" CDS 958..4983 /label=HER3 /note="note=""direct strand translation"" translation=""MRANDALQVLGLLFSLARGSEVGNSQAVCPGTLNGLSVTGDAENQ YQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWVREVTGYVLVAMNEFSTLPLPNLRV VRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDW RDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPN PNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQ YGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRF QTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNI QSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISA NRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQC LSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQC AHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGQTLV LIGKTHLTMALTVIAGLVVIFMMLGGTFLYWRGRRIQNKRAMRRYLERGESIEPLDPSE KANKVLARIFKETELRKLKVLGSGVFGTVHKGVWIPEGESIKIPVCIKVIEDKSGRQSF QAVTDHMLAIGSLDHAHIVRLLGLCPGSSLQLVTQYLPLGSLLDHVRQHRGALGPQLLL NWGVQIAKGMYYLEEHGMVHRNLAARNVLLKSPSQVQVADFGVADLLPPDDKQLLYSEA KTPIKWMALESIHFGKYTHQSDVWSYGVTVWELMTFGAEPYAGLRLAEVPDLLEKGERL AQPQICTIDVYMVMVKCWMIDENIRPTFKELANEFTRMARDPPRYLVIKRESGPGIAPG PEPHGLTNKKLEEVELEPELDLDLDLEAEEDNLATTTLGSALSLPVGTLNRPRGSQSLL SPSSGYMPMNQGNLGESCQESAVSGSSERCPRPVSLHPMPRGCLASESSEGHVTGSEAE LQEKVSMCRSRSRSRSPRPRGDSAYHSQRHSLLTPVTPLSPPGLEEEDVNGYVMPDTHL KGTPSSREGTLSSVGLSSVLGTEEEDEDEEYEYMNRRRRHSPPHPPRPSSLEELGYEYM DVGSDLSASLGSTQSCPLHPVPIMPTAGTTPDEDYEYMNRQRDGGGPGGDYAAMGACPA SERGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT" "" /note="color: #008080; direction: RIGHT" CDS 5029..5157 /label=DMVF181-C /note="note=""direct strand translation"" translation=""VVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQAK"" " /note="color: #008000; direction: RIGHT" primer_bind complement(5222..5239) /label=SP6 /note="color: #008b8b; direction: LEFT" terminator 5262..5489 /label=bGH PA /note="color: #ff8000; direction: RIGHT" misc_feature 6158..6235 /label=SV40 ORI /note="color: #4682b4; direction: RIGHT" CDS 6377..7168 /label=Kan/neoR /note="color: #8fbc8f; direction: RIGHT" primer_bind complement(7508..7528) /label=M13-rev /note="color: #008b8b; direction: LEFT" misc_binding complement(7534..7556) /label=LacO /note="color: #6495ed; direction: LEFT" promoter complement(7561..7590) /label=lac /note="color: #4682b4; direction: LEFT" rep_origin complement(7896..8524) /direction=LEFT /label=ColE1 origin /note="color: #4682b4" CDS complement(8676..9335) /label=AmpR /note="color: #8fbc8f; direction: LEFT" promoter complement(9575..9603) /label=Amp prom /note="color: #4682b4; direction: LEFT" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc 901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag cgccaccatg 961 agggcgaacg acgctctgca ggtgctgggc ttgcttttca gcctggcccg gggctccgag 1021 gtgggcaact ctcaggcagt gtgtcctggg actctgaatg gcctgagtgt gaccggcgat 1081 gctgagaacc aataccagac actgtacaag ctctacgaga ggtgtgaggt ggtgatgggg 1141 aaccttgaga ttgtgctcac gggacacaat gccgacctct ccttcctgca gtgggttcga 1201 gaagtgacag gctatgtcct cgtggccatg aatgaattct ctactctacc attgcccaac 1261 ctccgcgtgg tgcgagggac ccaggtctac gatgggaagt ttgccatctt cgtcatgttg 1321 aactataaca ccaactccag ccacgctctg cgccagctcc gcttgactca gctcaccgag 1381 attctgtcag ggggtgttta tattgagaag aacgataagc tttgtcacat ggacacaatt 1441 gactggaggg acatcgtgag ggaccgagat gctgagatag tggtgaagga caatggcaga 1501 agctgtcccc cctgtcatga ggtttgcaag gggcgatgct ggggtcctgg atcagaagac 1561 tgccagacat tgaccaagac catctgtgct cctcagtgta atggtcactg ctttgggccc 1621 aaccccaacc agtgctgcca tgatgagtgt gccgggggct gctcaggccc tcaggacaca 1681 gactgctttg cctgccggca cttcaatgac agtggagcct gtgtacctcg ctgtccacag 1741 cctcttgtct acaacaagct aactttccag ctggaaccca atccccacac caagtatcag 1801 tatggaggag tttgtgtagc cagctgtccc cataactttg tggtggatca aacatcctgt 1861 gtcagggcct gtcctcctga caagatggaa gtagataaaa atgggctcaa gatgtgtgag 1921 ccttgtgggg gactatgtcc caaagcctgt gagggaacag gctctgggag ccgcttccag 1981 actgtggact cgagcaacat tgatggattt gtgaactgca ccaagatcct gggcaacctg 2041 gactttctga tcaccggcct caatggagac ccctggcaca agatccctgc cctggaccca 2101 gagaagctca atgtcttccg gacagtacgg gagatcacag gttacctgaa catccagtcc 2161 tggccgcccc acatgcacaa cttcagtgtt ttttccaatt tgacaaccat tggaggcaga 2221 agcctctaca accggggctt ctcattgttg atcatgaaga acttgaatgt cacatctctg 2281 ggcttccgat ccctgaagga aattagtgct gggcgtatct atataagtgc caataggcag 2341 ctctgctacc accactcttt gaactggacc aaggtgcttc gggggcctac ggaagagcga 2401 ctagacatca agcataatcg gccgcgcaga gactgcgtgg cagagggcaa agtgtgtgac 2461 ccactgtgct cctctggggg atgctggggc ccaggccctg gtcagtgctt gtcctgtcga 2521 aattatagcc gaggaggtgt ctgtgtgacc cactgcaact ttctgaatgg ggagcctcga 2581 gaatttgccc atgaggccga atgcttctcc tgccacccgg aatgccaacc catggagggc 2641 actgccacat gcaatggctc gggctctgat acttgtgctc aatgtgccca ttttcgagat 2701 gggccccact gtgtgagcag ctgcccccat ggagtcctag gtgccaaggg cccaatctac 2761 aagtacccag atgttcagaa tgaatgtcgg ccctgccatg agaactgcac ccaggggtgt 2821 aaaggaccag agcttcaaga ctgtttagga caaacactgg tgctgatcgg caaaacccat 2881 ctgacaatgg ctttgacagt gatagcagga ttggtagtga ttttcatgat gctgggcggc 2941 acttttctct actggcgtgg gcgccggatt cagaataaaa gggctatgag gcgatacttg 3001 gaacggggtg agagcataga gcctctggac cccagtgaga aggctaacaa agtcttggcc 3061 agaatcttca aagagacaga gctaaggaag cttaaagtgc ttggctcggg tgtctttgga 3121 actgtgcaca aaggagtgtg gatccctgag ggtgaatcaa tcaagattcc agtctgcatt 3181 aaagtcattg aggacaagag tggacggcag agttttcaag ctgtgacaga tcatatgctg 3241 gccattggca gcctggacca tgcccacatt gtaaggctgc tgggactatg cccagggtca 3301 tctctgcagc ttgtcactca atatttgcct ctgggttctc tgctggatca tgtgagacaa 3361 caccgggggg cactggggcc acagctgctg ctcaactggg gagtacaaat tgccaaggga 3421 atgtactacc ttgaggaaca tggtatggtg catagaaacc tggctgcccg aaacgtgcta 3481 ctcaagtcac ccagtcaggt tcaggtggca gattttggtg tggctgacct gctgcctcct 3541 gatgataagc agctgctata cagtgaggcc aagactccaa ttaagtggat ggcccttgag 3601 agtatccact ttgggaaata cacacaccag agtgatgtct ggagctatgg tgtgacagtt 3661 tgggagttga tgaccttcgg ggcagagccc tatgcagggc tacgattggc tgaagtacca 3721 gacctgctag agaaggggga gcggttggca cagccccaga tctgcacaat tgatgtctac 3781 atggtgatgg tcaagtgttg gatgattgat gagaacattc gcccaacctt taaagaacta 3841 gccaatgagt tcaccaggat ggcccgagac ccaccacggt atctggtcat aaagagagag 3901 agtgggcctg gaatagcccc tgggccagag ccccatggtc tgacaaacaa gaagctagag 3961 gaagtagagc tggagccaga actagaccta gacctagact tggaagcaga ggaggacaac 4021 ctggcaacca ccacactggg ctccgccctc agcctaccag ttggaacact taatcggcca 4081 cgtgggagcc agagcctttt aagtccatca tctggataca tgcccatgaa ccagggtaat 4141 cttggggagt cttgccagga gtctgcagtt tctgggagca gtgaacggtg cccccgtcca 4201 gtctctctac acccaatgcc acggggatgc ctggcatcag agtcatcaga ggggcatgta 4261 acaggctctg aggctgagct ccaggagaaa gtgtcaatgt gtagaagccg gagtaggagc 4321 cggagcccac ggccacgcgg agatagcgcc taccattccc agcgccacag tctgctgact 4381 cctgttaccc cactctcccc acccgggtta gaggaagagg atgtcaacgg ttatgtcatg 4441 ccagatacac acctcaaagg tactccctcc tcccgggaag gcaccctttc ttcagtgggt 4501 ctcagttctg tcctgggtac tgaagaagaa gatgaagatg aggagtatga atacatgaac 4561 cggaggagaa ggcacagtcc acctcatccc cctaggccaa gttcccttga ggagctgggt 4621 tatgagtaca tggatgtggg gtcagacctc agtgcctctc tgggcagcac acagagttgc 4681 ccactccacc ctgtacccat catgcccact gcaggcacaa ctccagatga agactatgaa 4741 tatatgaatc ggcaacgaga tggaggtggt cctgggggtg attatgcagc catgggggcc 4801 tgcccagcat ctgagcgagg gtatgaagag atgagagctt ttcaggggcc tggacatcag 4861 gccccccatg tccattatgc ccgcctaaaa actctacgta gcttagaggc tacagactct 4921 gcctttgata accctgatta ctggcatagc aggcttttcc ccaaggctaa tgcccagaga 4981 acgggaggta gcggaggtag tggtaccgga ggtagcggag gtggaagtgt tgtccagttg 5041 ccagactatc actttgtgga ccaccacatt gagattaaaa gccacgacaa agattacagt 5101 aatgttaatc tgcatgagca tgccgaagcg cattctgagc tgccgaggca ggccaagtaa 5161 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggccctat 5221 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 5281 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 5341 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 5401 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 5461 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 5521 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 5581 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 5641 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt 5701 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 5761 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 5821 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 5881 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat 5941 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 6001 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 6061 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 6121 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 6181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 6241 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 6301 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 6361 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 6421 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 6481 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 6541 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 6601 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 6661 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 6721 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 6781 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 6841 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 6901 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 6961 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 7021 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 7081 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 7141 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 7201 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 7261 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 7321 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 7381 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 7441 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 7501 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 7561 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 7621 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 7681 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8101 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 9001 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 9061 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 9121 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 9181 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 9241 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 9301 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9361 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9421 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9481 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9541 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9601 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9661 cctgacgtc //