LOCUS AAV-U6gRNA-U6gRN 5969 bp DNA linear 30-JAN-2017 FEATURES Location/Qualifiers misc_feature 1623..2678 /note="iCre" misc_feature 883..1296 /note="gallus TnT promoter" misc_feature 440..521 /note="gRNA scaffold" misc_feature 795..876 /note="gRNA scaffold" misc_feature 163..411 /note="U6 Promoter" misc_feature 528..776 /note="U6 Promoter" misc_feature 778..793 /note="gRNA ins. site Sap1" misc_feature 416..435 /note="gRNA ins. site AarI" misc_feature 5970..6118 /note="ITR" misc_feature 3224..3372 /note="ITR" misc_feature 2714..3192 /note="PolyA - bovine growth hormone" misc_feature 1162..1181 /note="nv26-R" misc_feature 1396..1522 /note="b-globin intron" source 1..5969 /dnas_title="AAV-U6gRNA-U6gRNA-TnT-Cre " ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC 61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA 121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGCCGGTA CCGAGGGCCT ATTTCCCATG 181 ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG 241 ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG 301 TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA 361 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGACTGCAGG 421 TGGAATTCCA CCTGCTCAGG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC 481 GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TACGCGTGAG GGCCTATTTC 541 CCATGATTCC TTCATATTTG CATATACGAT ACAAGGCTGT TAGAGAGATA ATTGGAATTA 601 ATTTGACTGT AAACACAAAG ATATTAGTAC AAAATACGTG ACGTAGAAAG TAATAATTTC 661 TTGGGTAGTT TGCAGTTTTA AAATTATGTT TTAAAATGGA CTATCATATG CTTACCGTAA 721 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACACCGGAA 781 GAGCGAGCTC TTCTGTTTTA GAGCTAGAAA TAGCAAGTTA AAATAAGGCT AGTCCGTTAT 841 CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTTCTA GTGGGATAAA AGCAGTCTGG 901 GCTTTCACAT GACAGCATCT GGGGCTGCGG CAGAGGGTCG GGTCCGAAGC GCTGCCTTAT 961 CAGCGTCCCC AGCCCTGGGA GGTGACAGCT GGCTGGCTTG TGTCAGCCCC TCGGGCACTC 1021 ACGTATCTCC GTCCGACGGG TTTAAAATAG CAAAACTCTG AGGCCACACA ATAGCTTGGG 1081 CTTATATGGG CTCCTGTGGG GGAAGGGGGA GCACGGAGGG GGCCGGGGCC GCTGCTGCCA 1141 AAATAGCAGC TCACAAGTGT TGCATTCCTC TCTGGGCGCC GGGCACATTC CTGCTGGCTC 1201 TGCCCGCCCC GGGGTGGGCG CCGGGGGGAC CTTAAAGCCT CTGCCCCCCA AGGAGCCCTT 1261 CCCAGACAGC CGCCGGCACC CACCGCTCCG TGGGACGATC CCCGAAGCTC TAGAGCTTTA 1321 TTGCGGTAGT TTATCACAGT TAAATTGCTA ACGCAGTCAG TGCTTCTGAC ACAACAGTCT 1381 CGAACTTAAG CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA 1441 GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT 1501 TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC 1561 ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA CTATAGGCTA 1621 GCATGGTGCC CAAGAAGAAG AGGAAAGTCT CCAACCTGCT GACTGTGCAC CAAAACCTGC 1681 CTGCCCTCCC TGTGGATGCC ACCTCTGATG AAGTCAGGAA GAACCTGATG GACATGTTCA 1741 GGGACAGGCA GGCCTTCTCT GAACACACCT GGAAGATGCT CCTGTCTGTG TGCAGATCCT 1801 GGGCTGCCTG GTGCAAGCTG AACAACAGGA AATGGTTCCC TGCTGAACCT GAGGATGTGA 1861 GGGACTACCT CCTGTACCTG CAAGCCAGAG GCCTGGCTGT GAAGACCATC CAACAGCACC 1921 TGGGCCAGCT CAACATGCTG CACAGGAGAT CTGGCCTGCC TCGCCCTTCT GACTCCAATG 1981 CTGTGTCCCT GGTGATGAGG AGAATCAGAA AGGAGAATGT GGATGCTGGG GAGAGAGCCA 2041 AGCAGGCCCT GGCCTTTGAA CGCACTGACT TTGACCAAGT CAGATCCCTG ATGGAGAACT 2101 CTGACAGATG CCAGGACATC AGGAACCTGG CCTTCCTGGG CATTGCCTAC AACACCCTGC 2161 TGCGCATTGC CGAAATTGCC AGAATCAGAG TGAAGGACAT CTCCCGCACC GATGGTGGGA 2221 GAATGCTGAT CCACATTGGC AGGACCAAGA CCCTGGTGTC CACAGCTGGT GTGGAGAAGG 2281 CCCTGTCCCT GGGGGTTACC AAGCTGGTGG AGAGATGGAT CTCTGTGTCT GGTGTGGCTG 2341 ATGACCCCAA CAACTACCTG TTCTGCCGGG TCAGAAAGAA TGGTGTGGCT GCCCCTTCTG 2401 CCACCTCCCA ACTGTCCACC CGGGCCCTGG AAGGGATCTT TGAGGCCACC CACCGCCTGA 2461 TCTATGGTGC CAAGGATGAC TCTGGGCAGA GATACCTGGC CTGGTCTGGC CACTCTGCCA 2521 GAGTGGGTGC TGCCAGGGAC ATGGCCAGGG CTGGTGTGTC CATCCCTGAA ATCATGCAGG 2581 CTGGTGGCTG GACCAATGTG AACATAGTGA TGAACTACAT CAGAAACCTG GACTCTGAGA 2641 CTGGGGCCAT GGTGAGGCTG CTCGAGGATG GGGACTGAAA GCTTATCGAT ACCGAGCGCT 2701 GCTCGAGAGA TCTACGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 2761 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 2821 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 2881 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 2941 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 3001 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 3061 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 3121 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 3181 TTCCCTGTCC TTCTGATTTT GTAGGTAACC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC 3241 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC 3301 CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA 3361 GCTGCCTGCA GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC 3421 ACCGCATACG TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 3481 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 3541 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 3601 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 3661 TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 3721 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 3781 TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 3841 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT 3901 TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA 3961 CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG 4021 ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA 4081 ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 4141 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 4201 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 4261 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 4321 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 4381 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 4441 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 4501 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG 4561 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 4621 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 4681 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 4741 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 4801 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT 4861 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 4921 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 4981 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 5041 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 5101 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 5161 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 5221 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 5281 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 5341 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 5401 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 5461 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 5521 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 5581 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 5641 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 5701 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 5761 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 5821 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 5881 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 5941 GGCCTTTTGC TGGCCTTTTG CTCACATGT //