LOCUS       Exported                9158 bp ds-DNA     circular SYN 31-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 31, 2017 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /note="chicken beta-actin promoter"
     intron          662..1670
                     /note="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1742..2689
                     /codon_start=1
                     /note="ERT2"
                     /translation="MAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPI
                     LYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE
                     ILMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEE
                     FVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQR
                     LAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEE
                     TDQSHLATAGSTSSHSLQKYYITGEAEGFPATAVD"
     misc_feature    2696..3421
                     /note="PhoCl"
     misc_feature    3428..4408
                     /note="Cre"
     misc_feature    4415..5140
                     /note="PhoCl"
     misc_feature    5147..6091
                     /note="ERT2"
     polyA_site      6247..6302
                     /note="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(6661..6677)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6685..6701
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6709..6739)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6754..6775
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6834..7029
                     /note="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      6880..7015
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     polyA_signal    7035..7169
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7408..7996)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8167..9027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9028..9132)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcccgggt gagccgccac
     1741 catggctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
     1801 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
     1861 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
     1921 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
     1981 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
     2041 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
     2101 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
     2161 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
     2221 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
     2281 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
     2341 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
     2401 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
     2461 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
     2521 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
     2581 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
     2641 gtattacatc acgggggagg cagagggttt ccctgccaca gctgtcgacg gtaccgtgat
     2701 ccctgactac ttcaagcaga gcttccccga gggctacagc tgggagcgca gcatgaccta
     2761 cgaggacggc ggcatctgca tcgccaccaa cgacatcaca atggaggggg acagcttcat
     2821 caacaagatc cacttcaagg gcacgaactt cccccccaac ggccccgtga tgcagaagag
     2881 gaccgtgggc tgggaggcca gcaccgagaa gatgtacgag cgcgacggcg tgctgaaggg
     2941 cgacgtgaag atgaagctgc tgctgaaggg cggcggccac tatcgctgcg actaccgcac
     3001 cacctacaag gtcaagcaga agcccgtaaa gctgcccgac taccacttcg tggaccaccg
     3061 catcgagatc ctgagccacg acaaggacta caacaaggtg aagctgtacg agcacgccgt
     3121 ggcccgcaac tccaccgaca gcatggacga gctgtacaag ggtggcagcg gtggcatggt
     3181 gagcaagggc gaggagacca ttacaagcgt gatcaagcct gacatgaaga acaagctgcg
     3241 catggagggc aacgtgaacg gccacgcctt cgtgatcgag ggcgagggca gcggcaagcc
     3301 cttcgagggc atccagacga ttgatttgga ggtgaaggag ggcgccccgc tgcccttcgc
     3361 ctacgacatc ctgaccaccg ccttccacta cggcaaccgc gtgttcacca agtacccacg
     3421 ggctagcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg
     3481 ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc
     3541 atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta
     3601 tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca
     3661 gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc
     3721 actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc
     3781 tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg
     3841 ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat
     3901 agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt
     3961 aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag
     4021 cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc
     4081 gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag
     4141 ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg
     4201 cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg
     4261 agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg
     4321 ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc
     4381 aatggtgcgc ctgctggaag atggcgattc cggagtgatc cctgactact tcaagcagag
     4441 cttccccgag ggctacagct gggagcgcag catgacctac gaggacggcg gcatctgcat
     4501 cgccaccaac gacatcacaa tggaggggga cagcttcatc aacaagatcc acttcaaggg
     4561 cacgaacttc ccccccaacg gccccgtgat gcagaagagg accgtgggct gggaggccag
     4621 caccgagaag atgtacgagc gcgacggcgt gctgaagggc gacgtgaaga tgaagctgct
     4681 gctgaagggc ggcggccact atcgctgcga ctaccgcacc acctacaagg tcaagcagaa
     4741 gcccgtaaag ctgcccgact accacttcgt ggaccaccgc atcgagatcc tgagccacga
     4801 caaggactac aacaaggtga agctgtacga gcacgccgtg gcccgcaact ccaccgacag
     4861 catggacgag ctgtacaagg gtggcagcgg tggcatggtg agcaagggcg aggagaccat
     4921 tacaagcgtg atcaagcctg acatgaagaa caagctgcgc atggagggca acgtgaacgg
     4981 ccacgccttc gtgatcgagg gcgagggcag cggcaagccc ttcgagggca tccagacgat
     5041 tgatttggag gtgaaggagg gcgccccgct gcccttcgcc tacgacatcc tgaccaccgc
     5101 cttccactac ggcaaccgcg tgttcaccaa gtacccacgg ctcgagccat ctgctggaga
     5161 catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag
     5221 cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc
     5281 catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt
     5341 actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc
     5401 aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga
     5461 gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc
     5521 tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt
     5581 cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt
     5641 tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac
     5701 cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac
     5761 tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc
     5821 ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct
     5881 gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga
     5941 cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca
     6001 aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac
     6061 gggggaggca gagggtttcc ctgccacagc ttgatagcgg ccgcactcct caggtgcagg
     6121 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     6181 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     6241 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     6301 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
     6361 agagtttggc aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt
     6421 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     6481 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     6541 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     6601 gctgtccctc ttctcttatg aagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     6661 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6721 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6781 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     6841 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6901 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6961 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7021 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7081 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7141 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     7201 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     7621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8461 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8761 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9121 aggggttccg cgcacatttc cccgaaaagt gccacctg
//
