LOCUS pLBH1_Gst_expression 9233 bp ds-DNA circular 22-SEP-2016 DEFINITION . KEYWORDS "creator:Doudna Lab" "marker:AmpR" FEATURES Location/Qualifiers misc_feature 5371..5373 /label="TEV Last aa" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 5380..8643 /label="Gst Cas9" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS complement(2258..2449) /label="rop" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 promoter complement(9202..9218) /label="tet promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 rep_origin 1240..1828 /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter complement(9196..9201) /label="tet promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 2014..2156 /label="bom" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter complement(9219..9224) /label="tet promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 5350..8698 /label="Gblock" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 209..277 /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter 104..208 /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 terminator 8783..8830 /label="T7 terminator" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 4198..5298 /label="MBP" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 4159..4188 /label="10xHis" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 278..1069 /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter 4010..4028 /label="T7 promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 5353..5373 /label="TEV" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 ORIGIN 1 TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT 61 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG 121 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT 181 GCTTCAATAA CATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT 241 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT 301 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG 361 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA 421 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG 481 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT 541 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC 601 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA 661 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT 721 ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT 781 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC 841 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA 901 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG 961 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG 1021 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA 1081 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA 1141 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA 1201 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 1261 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 1321 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 1381 TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 1441 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 1501 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 1561 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 1621 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 1681 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 1741 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 1801 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 1861 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA 1921 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG 1981 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA 2041 TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC 2101 GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC 2161 GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT 2221 ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC 2281 CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA 2341 TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC 2401 TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG 2461 TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA 2521 CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC 2581 TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG 2641 TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA 2701 ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA 2761 AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC 2821 GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG 2881 TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA 2941 TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG 3001 TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC 3061 CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG 3121 ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT 3181 CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT 3241 TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG 3301 CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT 3361 AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC 3421 GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT 3481 GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT 3541 CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG 3601 TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG 3661 CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC 3721 CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC 3781 CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC 3841 TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC 3901 CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC 3961 GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAATT AATACGACTC 4021 ACTATAGGGA GACCACAACG GTTTCCCTCT AGTGCCGGCT CCGGAGAGCT CTTTAATTAA 4081 GCGGCCGCCC TGCAGGACTC GAGTTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT 4141 ATACATATGA AATCTTCTCA CCATCACCAT CACCATCACC ATCACCATGG TTCTTCTATG 4201 AAAATCGAAG AAGGTAAACT GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC 4261 GCTGAAGTCG GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG 4321 GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC TGACATTATC 4381 TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG GCCTGTTGGC TGAAATCACC 4441 CCGGACAAAG CGTTCCAGGA CAAGCTGTAT CCGTTTACCT GGGATGCCGT ACGTTACAAC 4501 GGCAAGCTGA TTGCTTACCC GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT 4561 CTGCTGCCGA ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA 4621 GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC CTGGCCGCTG 4681 ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG GCAAGTACGA CATTAAAGAC 4741 GTGGGCGTGG ATAACGCTGG CGCGAAAGCG GGTCTGACCT TCCTGGTTGA CCTGATTAAA 4801 AACAAACACA TGAATGCAGA CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC 4861 GAAACAGCGA TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG 4921 AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC GTTCGTTGGC 4981 GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG AGCTGGCAAA AGAGTTCCTC 5041 GAAAACTATC TGCTGACTGA TGAAGGTCTG GAAGCGGTTA ATAAAGACAA ACCGCTGGGT 5101 GCCGTAGCGC TGAAGTCTTA CGAGGAAGAG TTGGCGAAAG ATCCACGTAT TGCCGCCACT 5161 ATGGAAAACG CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG 5221 TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT CGATGAAGCC 5281 CTGAAAGACG CGCAGACTAA TTCGAGCTCG AACAACAACA ACAATAACAA TAACAACAAC 5341 CTCGGGATCG AGGAAAACCT GTACTTCCAA TCCAATGCAA TGCGTTATAA GATTGGCCTG 5401 GACATCGGTA TTACCTCTGT TGGTTGGGCA GTCATGAACC TGGATATCCC TCGTATCGAA 5461 GATCTGGGCG TGCGCATTTT TGACCGTGCG GAGAACCCGC AGACCGGTGA ATCTCTGGCT 5521 CTGCCGCGTC GTCTGGCACG TAGCGCACGC CGCCGCCTGC GTCGTCGTAA ACACCGTCTG 5581 GAGCGTATTC GTCGCCTGGT TATTCGTGAA GGCATCCTGA CGAAAGAAGA ACTGGATAAA 5641 CTGTTCGAAG AAAAACACGA GATCGACGTA TGGCAGCTGC GTGTAGAAGC CCTGGACCGT 5701 AAGCTGAACA ACGACGAACT GGCGCGTGTC CTGCTGCATC TGGCAAAGCG TCGTGGCTTC 5761 AAATCTAACC GTAAATCTGA ACGCTCCAAT AAAGAGAACT CCACTATGCT GAAACATATT 5821 GAGGAGAACC GTGCAATTCT GTCTAGCTAC CGTACCGTGG GCGAAATGAT TGTTAAAGAC 5881 CCGAAATTCG CACTGCATAA GCGTAACAAA GGCGAAAACT ACACCAACAC CATTGCACGC 5941 GATGACCTGG AACGTGAAAT CCGTCTGATT TTCTCCAAAC AGCGCGAATT CGGCAACATG 6001 TCTTGCACCG AAGAATTCGA AAACGAATAT ATTACCATTT GGGCATCTCA GCGTCCGGTG 6061 GCGTCTAAAG ATGATATCGA AAAAAAAGTA GGCTTTTGTA CTTTCGAACC GAAGGAAAAA 6121 CGTGCGCCGA AAGCCACCTA TACCTTCCAG TCTTTTATCG CGTGGGAACA TATCAACAAA 6181 CTGCGTCTGA TTTCTCCGTC TGGCGCCCGC GGCCTGACCG ACGAAGAACG TCGTCTGCTG 6241 TATGAACAAG CATTCCAGAA AAACAAAATT ACCTACCACG ATATTCGTAC CCTGCTGCAT 6301 CTGCCGGACG ACACCTACTT CAAGGGCATC GTTTACGATC GCGGTGAATC TCGTAAGCAG 6361 AACGAAAACA TTCGTTTCCT GGAACTGGAT GCATACCACC AGATCCGTAA AGCTGTAGAT 6421 AAAGTTTACG GCAAGGGTAA ATCCAGCAGC TTCCTGCCGA TCGACTTTGA TACCTTCGGT 6481 TACGCGCTGA CCCTGTTTAA AGACGATGCG GATATCCACT CTTACCTGCG CAACGAGTAC 6541 GAACAGAACG GCAAACGTAT GCCTAACCTG GCTAACAAAG TTTACGATAA CGAGCTGATT 6601 GAAGAACTGC TGAACCTGTC CTTCACTAAA TTCGGTCACC TGTCTCTGAA AGCTCTGCGT 6661 TCCATCCTGC CGTATATGGA ACAGGGTGAA GTCTACTCCT CCGCTTGTGA ACGTGCAGGC 6721 TACACCTTCA CCGGTCCGAA AAAGAAGCAA AAAACTATGC TGCTGCCGAA CATCCCGCCG 6781 ATTGCGAACC CTGTAGTAAT GCGTGCACTG ACCCAGGCGC GCAAAGTAGT CAACGCGATC 6841 ATCAAAAAGT ACGGCAGCCC GGTTTCCATC CATATCGAAC TGGCGCGCGA CCTGAGCCAG 6901 ACTTTTGACG AGCGTCGTAA AACTAAAAAG GAACAGGATG AAAACCGTAA AAAAAACGAA 6961 ACCGCGATCC GCCAGCTGAT GGAATACGGT CTGACTCTGA ACCCTACTGG TCACGATATT 7021 GTGAAGTTCA AGCTGTGGTC TGAACAGAAC GGTCGCTGTG CTTACTCTCT GCAGCCGATC 7081 GAGATCGAAC GTCTGCTGGA GCCAGGTTAC GTTGAAGTAG ATCATGTGAT CCCGTACTCC 7141 CGCTCTCTGG ATGATTCTTA TACCAACAAA GTTCTGGTTC TGACTCGCGA AAACCGTGAG 7201 AAAGGCAACC GCATCCCAGC TGAATATCTG GGTGTTGGCA CTGAGCGTTG GCAACAGTTC 7261 GAAACCTTCG TCCTGACCAA TAAACAGTTC TCTAAAAAGA AACGTGACCG TCTGCTGCGT 7321 CTGCACTACG ATGAAAACGA AGAGACTGAA TTCAAAAACC GTAACCTGAA CGATACTCGC 7381 TACATCAGCC GCTTCTTCGC AAACTTCATT CGTGAACACC TGAAATTTGC GGAATCCGAC 7441 GATAAACAGA AAGTTTATAC CGTAAACGGC CGTGTTACCG CCCACCTGCG TTCTCGCTGG 7501 GAGTTCAACA AGAACCGTGA GGAAAGCGAT CTGCACCACG CTGTTGACGC CGTTATTGTG 7561 GCGTGCACCA CCCCAAGCGA TATCGCTAAG GTGACCGCAT TCTACCAGCG TCGTGAGCAG 7621 AACAAGGAAC TGGCCAAAAA AACCGAACCG CATTTTCCGC AGCCGTGGCC GCACTTCGCG 7681 GACGAACTGC GTGCTCGTCT GTCCAAACAT CCTAAAGAAA GCATCAAAGC TCTGAACCTG 7741 GGTAACTACG ATGACCAAAA ACTGGAATCT CTGCAGCCGG TGTTTGTCAG CCGTATGCCG 7801 AAACGTTCTG TTACTGGCGC TGCGCACCAG GAAACGCTGC GCCGTTACGT GGGCATCGAC 7861 GAACGCTCCG GTAAAATCCA GACCGTAGTA AAAACCAAAC TGTCCGAGAT TAAACTGGAT 7921 GCATCCGGCC ACTTCCCAAT GTACGGTAAA GAATCCGATC CACGCACTTA TGAAGCCATC 7981 CGCCAGCGTC TGCTGGAGCA TAACAACGAC CCGAAAAAGG CATTCCAGGA GCCTCTGTAC 8041 AAACCGAAAA AAAACGGCGA ACCGGGCCCG GTAATCCGTA CTGTAAAAAT TATCGACACG 8101 AAAAACCAGG TGATCCCTCT GAACGATGGT AAAACCGTGG CCTACAATTC CAACATCGTT 8161 CGCGTGGACG TGTTCGAAAA AGATGGTAAA TACTACTGTG TACCGGTGTA TACCATGGAC 8221 ATCATGAAAG GCATTCTGCC GAACAAAGCG ATTGAACCGA ACAAGCCGTA CTCTGAATGG 8281 AAAGAAATGA CCGAAGATTA CACGTTTCGT TTCAGCCTGT ATCCGAACGA CCTGATCCGC 8341 ATCGAACTGC CGCGTGAAAA AACCGTTAAA ACCGCTGCAG GCGAAGAAAT TAACGTGAAA 8401 GACGTGTTCG TTTACTATAA AACGATCGAC TCCGCAAACG GCGGCCTGGA ACTGATTTCT 8461 CACGACCACC GTTTCTCTCT GCGTGGCGTT GGCTCTCGCA CCCTGAAACG TTTCGAGAAA 8521 TATCAAGTTG ATGTTCTGGG TAACATCTAT AAAGTGCGTG GCGAGAAACG TGTCGGTCTG 8581 GCGTCCTCCG CACACAGCAA ACCTGGCAAA ACCATTCGTC CACTGCAATC TACTCGTGAC 8641 TAATAACATT GGAAGTGGAT AACGGATCCG CGATCGCGGC GCGCCACCTG GTGGCCGGCC 8701 GGTACCACGC GTGCGCGCTG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC 8761 TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG 8821 GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA 8881 TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA 8941 AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG 9001 CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC 9061 CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA 9121 TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT 9181 GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA //