LOCUS       pcDNA3-FLINC-AKAR1-CAAX.xdna    7719 bp      DNA     circular   1/31/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             1009..1680
                     /label=Dronpa
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2515..3207
                     /label=TagRFP-T
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5558..5578)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3272..3289)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5946..6574)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5584..5606)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6726..7385)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4427..5218
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7625..7653)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5611..5640)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4208..4285
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3312..3539
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2122..2469
                     /label=EV Linker
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1693..2115
                     /label=FHA1
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2476..2499
                     /label=PKAsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3214..3252
                     /label=Kras-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCCAT GAGTGTGATT 
     1021 AAACCAGACA TGAAGATCAA GCTGCGTATG GAAGGCGCTG TAAATGGACA CCCGTTCGCG 
     1081 ATTGAAGGAG TTGGCCTTGG GAAGCCTTTC GAGGGAAAAC AGAGTATGGA CCTTAAAGTC 
     1141 AAAGAAGGCG GACCTCTGCC TTTCGCCTAT GACATCTTGA CAACTGTGTT CTGTTACGGC 
     1201 AACAGGGTAT TCGCCAAATA CCCAGAAAAT ATAGTAGACT ATTTCAAGCA GTCGTTTCCT 
     1261 GAGGGCTACT CTTGGGAACG AAGCATGAAT TACGAAGACG GGGGCATTTG TAACGCGACA 
     1321 AACGACATAA CCCTGGATGG TGACTGTTAT ATCTATGAAA TTCGATTTGA TGGTGTGAAC 
     1381 TTTCCTGCCA ATGGTCCAGT TATGCAGAAG AGGACTGTGA AATGGGAGCC ATCCACTGAG 
     1441 AAATTGTATG TGCGTGATGG AGTGCTGAAG GGTGATGTTA ACATGGCTCT GTCGCTTGAA 
     1501 GGAGGTGGCC ATTACCGATG TGACTTCAAA ACTACTTATA AAGCTAAGAA GGTTGTCCAG 
     1561 TTGCCAGACT ATCACTTTGT GGACCACCAC ATTGAGATTA AAAGCCACGA CAAAGATTAC 
     1621 AGTAATGTTA ATCTGCATGA GCATGCCGAA GCGCATTCTG AGCTGCCGAG GCAGGCCAAG 
     1681 AGATCTATGC ATAAGTTTTC TCAAGAACAG ATCGGCGAAA ACATTGTGTG CAGGGTCATT 
     1741 TGTACCACGG GTCAAATTCC CATCCGAGAT TTGTCAGCTG ATATTTCACA AGTGCTTAAG 
     1801 GAAAAACGAT CCATAAAGAA AGTTTGGACA TTTGGTAGAA ACCCAGCCTG TGACTATCAT 
     1861 TTAGGAAACA TTTCAAGACT GTCAAATAAG CATTTCCAAA TACTACTAGG AGAAGACGGT 
     1921 AACCTTTTAT TGAATGACAT TTCCACTAAT GGGACCTGGT TAAATGGGCA AAAAGTCGAG 
     1981 AAGAACAGCA ATCAGTTACT GTCCCAAGGT GATGAAATAA CCGTTGGTGT AGGCGTGGAA 
     2041 TCAGATATTT TATCTCTGGT CATTTTCATA AACGACAAAT TTAAGCAGTG CCTGGAGCAG 
     2101 AACAAAGTTG ATCGCGGTAC CAGTGCTGGT GGTAGTGCTG GTGGTAGTGC TGGTGGTAGT 
     2161 GCTGGTGGTA GTGCTGGTGG TTCCGGCAGT GCTGGTGGTA GTGCTGGTGG TAGTACCAGT 
     2221 GCTGGTGGTA GTGCTGGTGG TAGTGCTGGT GGTAGTGCTG GTGGTAGTGC TGGTGGTTCC 
     2281 GGCAGTGCTG GTGGTAGTGC TGGTGGTAGT ACCAGTGCTG GTGGTAGTGC TGGTGGTAGT 
     2341 GCTGGTGGTA GTGCTGGTGG TAGTGCTGGT GGTTCCGGCA GTGCTGGTGG TAGTGCTGGT 
     2401 GGTAGTACCA GTGCTGGTGG TAGTGCTGGT GGTAGTGCTG GTGGTAGTGC TGGTGGTAGT 
     2461 GCTGGTGGTT CCGGATTGAG GCGCGCGACG CTGGTTGACG GCGGCAGCGA GCTCATGAGC 
     2521 GAGCTGATTA AGGAGAACAT GCACATGAAG CTGTACATGG AGGGCACCGT GAACAACCAC 
     2581 CACTTCAAGT GCACATCCGA GGGCGAAGGC AAGCCCTACG AGGGCACCCA GACCATGAGA 
     2641 ATCAAGGTGG TCGAGGGCGG CCCTCTCCCC TTCGCCTTCG ACATCCTGGC TACCAGCTTC 
     2701 ATGTACGGCA GCAGAACCTT CATCAACCAC ACCCAGGGCA TCCCCGATTT CTTTAAGCAG 
     2761 TCCTTCCCTG AGGGCTTCAC ATGGGAGAGA GTCACCACAT ACGAAGACGG GGGCGTGCTG 
     2821 ACCGCTACCC AGGACACCAG CCTCCAGGAC GGCTGCCTCA TCTACAACGT CAAGATCAGA 
     2881 GGGGTGAACT TCCCATCCAA CGGCCCTGTG ATGCAGAAGA AAACACTCGG CTGGGAGGCC 
     2941 AACACCGAGA TGCTGTACCC CGCTGACGGC GGCCTGGAAG GCAGAACCGA CATGGCCCTG 
     3001 AAGCTCGTGG GCGGGGGCCA CCTGATCTGC AACTTCAAGA CCACATACAG ATCCAAGAAA 
     3061 CCCGCTAAGA ACCTCAAGAT GCCCGGCGTC TACTATGTGG ACCACAGACT GGAAAGAATC 
     3121 AAGGAGGCCG ACAAAGAGAC CTACGTCGAG CAGCACGAGG TGGCTGTGGC CAGATACTGC 
     3181 GACCTCCCTA GCAAACTGGG GCACAAGGAA TTCAAGAAGA AGAAGAAGAG CAAGACCAAG 
     3241 TGCGTGATCA TGTAATCTAG AGGGCCCTAT Tctatagtgt cacctaaatg ctagagctcg 
     3301 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 
     3361 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 
     3421 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 
     3481 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 
     3541 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 
     3601 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 
     3661 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 
     3721 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct 
     3781 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 
     3841 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 
     3901 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat 
     3961 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 
     4021 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 
     4081 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
     4141 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     4201 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     4261 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 
     4321 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 
     4381 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 
     4441 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 
     4501 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 
     4561 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 
     4621 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 
     4681 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 
     4741 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 
     4801 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 
     4861 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 
     4921 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 
     4981 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 
     5041 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 
     5101 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 
     5161 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 
     5221 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 
     5281 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 
     5341 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 
     5401 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     5461 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     5521 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 
     5581 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
     5641 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     5701 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
     5761 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     5821 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     5881 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     5941 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     6001 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     6061 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     6121 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 
     6181 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     6241 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     6301 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     6361 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     6421 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     6481 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     6541 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     6601 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     6661 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     6721 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     6781 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     6841 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     6901 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     6961 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     7021 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     7081 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     7141 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     7201 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     7261 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     7321 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     7381 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     7441 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     7501 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     7561 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     7621 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     7681 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//