LOCUS       pcDNA3-Lyn-FLINC-EKAR1.xdna    7410 bp      DNA     circular   1/31/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             946..1617
                     /label=Dronpa
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2206..2898
                     /label=TagRFP-T
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5249..5269)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(2963..2980)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5637..6265)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5275..5297)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6417..7076)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4118..4909
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7316..7344)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5302..5331)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3899..3976
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3003..3230
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1792..2139
                     /label=EV Linker
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1624..1785
                     /label=WW_Domain
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2146..2199
                     /label=ERKsub_DD
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     sig_peptide     907..939
                     /label=Lyn-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCaa gcttGCGGCC 
      901 GCCACCATGG GCTGCATCAA GAGCAAGCGC AAGGACAAGG ATCCGATGAG TGTGATTAAA 
      961 CCAGACATGA AGATCAAGCT GCGTATGGAA GGCGCTGTAA ATGGACACCC GTTCGCGATT 
     1021 GAAGGAGTTG GCCTTGGGAA GCCTTTCGAG GGAAAACAGA GTATGGACCT TAAAGTCAAA 
     1081 GAAGGCGGAC CTCTGCCTTT CGCCTATGAC ATCTTGACAA CTGTGTTCTG TTACGGCAAC 
     1141 AGGGTATTCG CCAAATACCC AGAAAATATA GTAGACTATT TCAAGCAGTC GTTTCCTGAG 
     1201 GGCTACTCTT GGGAACGAAG CATGAATTAC GAAGACGGGG GCATTTGTAA CGCGACAAAC 
     1261 GACATAACCC TGGATGGTGA CTGTTATATC TATGAAATTC GATTTGATGG TGTGAACTTT 
     1321 CCTGCCAATG GTCCAGTTAT GCAGAAGAGG ACTGTGAAAT GGGAGCCATC CACTGAGAAA 
     1381 TTGTATGTGC GTGATGGAGT GCTGAAGGGT GATGTTAACA TGGCTCTGTC GCTTGAAGGA 
     1441 GGTGGCCATT ACCGATGTGA CTTCAAAACT ACTTATAAAG CTAAGAAGGT TGTCCAGTTG 
     1501 CCAGACTATC ACTTTGTGGA CCACCACATT GAGATTAAAA GCCACGACAA AGATTACAGT 
     1561 AATGTTAATC TGCATGAGCA TGCCGAAGCG CATTCTGAGC TGCCGAGGCA GGCCAAGaga 
     1621 tctATGGCGG ACGAGGAGAA GCTGCCGCCC GGCTGGGAGA AGCGCATGAG CCGCAGCTCA 
     1681 GGCCGAGTGT ACTACTTCAA CCACATCACT AACGCCAGCC AGTGGGAGCG GCCCAGCGGC 
     1741 AACAGCAGCA GTGGTGGCAA AAACGGGCAG GGGGAGCCTG CCAGGggtac cAGTGCTGGT 
     1801 GGTAGTGCTG GTGGTAGTGC TGGTGGTAGT GCTGGTGGTA GTGCTGGTGG TtccggcAGT 
     1861 GCTGGTGGTA GTGCTGGTGG TagtaccAGT GCTGGTGGTA GTGCTGGTGG TAGTGCTGGT 
     1921 GGTAGTGCTG GTGGTAGTGC TGGTGGTtcc ggcAGTGCTG GTGGTAGTGC TGGTGGTagt 
     1981 accAGTGCTG GTGGTAGTGC TGGTGGTAGT GCTGGTGGTA GTGCTGGTGG TAGTGCTGGT 
     2041 GGTtccggcA GTGCTGGTGG TAGTGCTGGT GGTagtaccA GTGCTGGTGG TAGTGCTGGT 
     2101 GGTAGTGCTG GTGGTAGTGC TGGTGGTAGT GCTGGTGGTt ccggaCCAGA TGTCCCTAGA 
     2161 ACTCCAGTGG ATAAAgcaaa gctgtcaTTC CAATTTCCGg gtaccATGAG CGAGCTGATT 
     2221 AAGGAGAACA TGCACATGAA GCTGTACATG GAGGGCACCG TGAACAACCA CCACTTCAAG 
     2281 TGCACATCCG AGGGCGAAGG CAAGCCCTAC GAGGGCACCC AGACCATGAG AATCAAGGTG 
     2341 GTCGAGGGCG GCCCTCTCCC CTTCGCCTTC GACATCCTGG CTACCAGCTT CATGTACGGC 
     2401 AGCAGAACCT TCATCAACCA CACCCAGGGC ATCCCCGATT TCTTTAAGCA GTCCTTCCCT 
     2461 GAGGGCTTCA CATGGGAGAG AGTCACCACA TACGAAGACG GGGGCGTGCT GACCGCTACC 
     2521 CAGGACACCA GCCTCCAGGA CGGCTGCCTC ATCTACAACG TCAAGATCAG AGGGGTGAAC 
     2581 TTCCCATCCA ACGGCCCTGT GATGCAGAAG AAAACACTCG GCTGGGAGGC CAACACCGAG 
     2641 ATGCTGTACC CCGCTGACGG CGGCCTGGAA GGCAGAACCG ACATGGCCCT GAAGCTCGTG 
     2701 GGCGGGGGCC ACCTGATCTG CAACTTCAAG ACCACATACA GATCCAAGAA ACCCGCTAAG 
     2761 AACCTCAAGA TGCCCGGCGT CTACTATGTG GACCACAGAC TGGAAAGAAT CAAGGAGGCC 
     2821 GACAAAGAGA CCTACGTCGA GCAGCACGAG GTGGCTGTGG CCAGATACTG CGACCTCCCT 
     2881 AGCAAACTGG GGCACAAGta agaattcTGC AGATATCCAT CACACTGGCG GCCGCTCGAG 
     2941 CATGCATCTA GAGGGCCCTA TTctatagtg tcacctaaat gctagagctc gctgatcagc 
     3001 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 
     3061 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 
     3121 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 
     3181 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc 
     3241 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag 
     3301 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 
     3361 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 
     3421 tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 
     3481 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 
     3541 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 
     3601 actcaaccct atctcggtct attcttttga tttataaggg attttgggga tttcggccta 
     3661 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg 
     3721 tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta tgcaaagcat 
     3781 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 
     3841 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 
     3901 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 
     3961 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 
     4021 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 
     4081 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 
     4141 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 
     4201 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 
     4261 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 
     4321 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 
     4381 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 
     4441 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 
     4501 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 
     4561 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 
     4621 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 
     4681 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 
     4741 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 
     4801 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 
     4861 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 
     4921 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 
     4981 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 
     5041 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 
     5101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     5161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 
     5221 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 
     5281 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 
     5341 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 
     5401 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 
     5461 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     5521 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     5581 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     5641 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     5701 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     5761 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     5821 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 
     5881 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     5941 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     6001 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     6061 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
     6121 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     6181 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     6241 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     6301 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     6361 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     6421 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     6481 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     6541 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     6601 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     6661 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     6721 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     6781 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     6841 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     6901 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     6961 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     7021 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     7081 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     7141 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     7201 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     7261 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     7321 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
     7381 acatttcccc gaaaagtgcc acctgacgtc 
//