LOCUS       pcDNA3-PKAsub-TagRFPT-CAAX.xdna    6255 bp      DNA     circular   1/31/17
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             1051..1743
                     /label=TagRFP-T
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4094..4114)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(1808..1825)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4482..5110)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4120..4142)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5262..5921)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2963..3754
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6161..6189)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4147..4176)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2744..2821
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      1848..2075
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1012..1035
                     /label=PKAsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1750..1788
                     /label=Kras-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCCGG ATTGAGGCGC 
     1021 GCGACGCTGG TTGACGGCGG CAGCGAGCTC ATGAGCGAGC TGATTAAGGA GAACATGCAC 
     1081 ATGAAGCTGT ACATGGAGGG CACCGTGAAC AACCACCACT TCAAGTGCAC ATCCGAGGGC 
     1141 GAAGGCAAGC CCTACGAGGG CACCCAGACC ATGAGAATCA AGGTGGTCGA GGGCGGCCCT 
     1201 CTCCCCTTCG CCTTCGACAT CCTGGCTACC AGCTTCATGT ACGGCAGCAG AACCTTCATC 
     1261 AACCACACCC AGGGCATCCC CGATTTCTTT AAGCAGTCCT TCCCTGAGGG CTTCACATGG 
     1321 GAGAGAGTCA CCACATACGA AGACGGGGGC GTGCTGACCG CTACCCAGGA CACCAGCCTC 
     1381 CAGGACGGCT GCCTCATCTA CAACGTCAAG ATCAGAGGGG TGAACTTCCC ATCCAACGGC 
     1441 CCTGTGATGC AGAAGAAAAC ACTCGGCTGG GAGGCCAACA CCGAGATGCT GTACCCCGCT 
     1501 GACGGCGGCC TGGAAGGCAG AACCGACATG GCCCTGAAGC TCGTGGGCGG GGGCCACCTG 
     1561 ATCTGCAACT TCAAGACCAC ATACAGATCC AAGAAACCCG CTAAGAACCT CAAGATGCCC 
     1621 GGCGTCTACT ATGTGGACCA CAGACTGGAA AGAATCAAGG AGGCCGACAA AGAGACCTAC 
     1681 GTCGAGCAGC ACGAGGTGGC TGTGGCCAGA TACTGCGACC TCCCTAGCAA ACTGGGGCAC 
     1741 AAGGAATTCA AGAAGAAGAA GAAGAGCAAG ACCAAGTGCG TGATCATGTA ATCTAGAGGG 
     1801 CCCTATTcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc 
     1861 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 
     1921 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 
     1981 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 
     2041 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 
     2101 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 
     2161 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 
     2221 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat 
     2281 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 
     2341 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 
     2401 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 
     2461 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga 
     2521 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 
     2581 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc 
     2641 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 
     2701 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 
     2761 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 
     2821 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 
     2881 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 
     2941 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 
     3001 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 
     3061 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 
     3121 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 
     3181 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 
     3241 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 
     3301 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 
     3361 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 
     3421 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 
     3481 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 
     3541 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 
     3601 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 
     3661 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 
     3721 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 
     3781 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 
     3841 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 
     3901 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 
     3961 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     4021 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 
     4081 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 
     4141 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 
     4201 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 
     4261 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 
     4321 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     4381 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     4441 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     4501 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     4561 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     4621 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     4681 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     4741 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     4801 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     4861 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     4921 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     4981 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     5041 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     5101 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     5161 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     5221 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     5281 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     5341 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     5401 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     5461 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     5521 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     5581 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     5641 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     5701 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     5761 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     5821 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     5881 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     5941 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     6001 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     6061 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     6121 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     6181 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     6241 gtgccacctg acgtc
//