LOCUS       Exported                6765 bp ds-DNA     circular SYN 01-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pET151-Vps4p(101-437)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 31, 2017 from SnapGene 3.3.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     209..228
                     /note="T7"
     misc_feature    228..252
                     /note="lacO"
     misc_binding    228..250
                     /note="LacO"
     CDS             300..317
                     /note="6His"
     CDS             318..359
                     /note="V5 tag"
     CDS             360..380
                     /note="TEV site"
     misc_feature    381..1394
                     /note="Vps4p(101-437)"
     promoter        1883..1911
                     /note="Amp prom"
     CDS             2151..2810
                     /note="AmpR"
     rep_origin      2962..3590
                     /direction=RIGHT
                     /note="ColE1 origin"
     misc_feature    complement(5505..6596)
                     /note="lacI"
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgc
      301 atcatcacca tcaccatggt aagcctatcc ctaaccctct cctcggtctc gattctacgg
      361 aaaacctgta ttttcaggga caggaagagg gtgaggataa tggtggagaa gacaacaaaa
      421 agctaagagg tgccttatcg agtgctattt tgtctgagaa gcccaatgtt aagtgggaag
      481 acgtggctgg actggaagga gccaaagagg cccttaaaga agccgttatt ttacctgtca
      541 agttcccaca tttgttcaaa ggtaaccgta agcccacttc gggaatctta ttgtacggac
      601 caccaggtac aggtaaatca tatttggcaa aagcagtggc caccgaggcc aattctacct
      661 ttttcagtgt tagttccagt gatttggttt ctaagtggat gggtgaatct gaaaaacttg
      721 tcaaacagtt atttgcgatg gcaagagaaa ataaaccttc tattattttt atagatgaag
      781 tggatgcgct aacaggtacc agaggagaag gagagagcga agcaagtaga agaattaaaa
      841 cagaattatt ggtacaaatg aatggtgttg ggaatgactc ccaaggtgtt ttggtccttg
      901 gcgcaacaaa tatcccatgg caactggaca gtgccatcag gagaagattt gaaagaagaa
      961 tatatatacc attaccagat ttggcggcaa gaaccaccat gttcgagatt aacgtcggcg
     1021 acactccatg cgtattgacc aaggaagatt atagaacttt aggtgcaatg actgaaggtt
     1081 attccgggag tgatattgct gtagtggtga aagatgcgct aatgcaaccg ataagaaaaa
     1141 ttcaaagcgc gacacatttc aaagatgtct ctacggagga cgacgaaaca agaaaactaa
     1201 caccatgctc tccaggtgat gatggcgcca tcgagatgag ttggacggat attgaagctg
     1261 atgaattaaa agaaccagat ctcaccataa aggatttctt aaaggctatc aaatcgacga
     1321 gacctaccgt gaatgaggat gacttgctga agcaagaaca gttcactaga gattttggtc
     1381 aagaaggtaa ctagcatatg aagggcgagc tcagatccgg ctgctaacaa agcccgaaag
     1441 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     1501 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga
     1561 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     1621 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
     1681 actgtgataa actaccgcat taaagctagc ttatcgatga taagctgtca aacatgagaa
     1741 ttaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     1801 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2161 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2461 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     2521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2641 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3121 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     3781 cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga
     3841 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     3901 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3961 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4021 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     4081 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     4141 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     4201 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     4261 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     4321 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     4381 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     4441 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     4501 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     4561 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     4621 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc
     4681 gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg
     4741 ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg
     4801 aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac
     4861 cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc
     4921 cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg
     4981 aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta
     5041 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     5101 tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg
     5161 cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac
     5221 cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga
     5281 tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca
     5341 cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc
     5401 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
     5461 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc
     5521 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5581 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
     5641 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
     5701 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
     5761 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
     5821 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
     5881 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
     5941 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
     6001 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
     6061 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
     6121 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
     6181 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
     6241 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
     6301 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
     6361 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
     6421 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
     6481 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
     6541 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     6601 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
     6661 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     6721 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatg
//
