LOCUS       Exported                5984 bp ds-DNA     circular SYN 30-JAN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCA24N-Ligase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 31, 2017 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        71..163
                     /note="T5-lac promoter"
     CDS             185..235
                     /codon_start=1
                     /note="His-tag"
                     /translation="MRGSHHHHHHTDPALRA"
     CDS             236..1696
                     /codon_start=1
                     /note="T4 ligase gene"
                     /translation="ILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQYY
                     IKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVL
                     RRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGAR
                     CFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQ
                     VKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVW
                     DYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQ
                     GLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGK
                     IKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLP
                     IAIRLREDKTKANTFEDVFGDFHEVTGL"
     CDS             1697..1714
                     /codon_start=1
                     /note="Ext. (ex-GFP)"
                     /translation="GLCGR"
     terminator      1882..2063
                     /note="rrnB terminator"
     CDS             2505..3164
                     /codon_start=1
                     /note="CAT gene"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      4299
                     /direction=RIGHT
                     /note="ori"
     CDS             4622..5704
                     /codon_start=1
                     /note="LacIq"
                     /translation="CETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGA
                     ELHSQPRGTTTGGQTVVADWRCHLQSGPARAVANCRGD*ISRRSTGCQRGGVDGRTKRR
                     RSL*SGGAQSSRATRQWADH*LSAG*PGCHCCGSCLH*CSGVIS*CL*PDTHQQYYFLP
                     *RRYATGRGASGRIGSPANRAVSGPIKFCLGASASGWLA*ISHSQSNSADSGTGRRLEC
                     HVRFSTNHANAE*GHRSHCDAGCQRSDGAGRNARHYRVRAARWCGYLGSGIRRYRRQLM
                     LYPAVNHHQTGFSPAGANQRGPLAATLSGPGGEGQSAVARLTGEKKNHPGAQYANRLSP
                     RVGRFINAAGTTGFPTGKRAV"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactatgaga ggatctcacc atcaccatca ccatacggat ccggccctga gggccattct
      241 taaaattctg aacgaaatag catctattgg ttcaactaaa cagaagcaag caattcttga
      301 aaagaataaa gataatgaat tgcttaaacg agtatatcgt ctgacttatt ctcgtgggtt
      361 acagtattat atcaagaaat ggcctaaacc tggtattgct acccagagtt ttggaatgtt
      421 gactcttacc gatatgcttg acttcattga attcacatta gctactcgga aattgactgg
      481 aaatgcagca attgaggaat taactggata tatcaccgat ggtaaaaaag atgatgttga
      541 agttttgcgt cgagtgatga tgcgagacct tgaatgtggt gcttcagtat ctattgcaaa
      601 caaagtttgg ccaggtttaa ttcctgaaca acctcaaatg ctcgcaagtt cttatgatga
      661 aaaaggcatt aataagaata tcaaatttcc agcctttgct cagttaaaag ctgatggagc
      721 tcggtgtttt gctgaagtta gaggtgatga attagatgat gttcgtcttt tatcacgagc
      781 tggtaatgaa tatctaggat tagatcttct taaggaagag ttaattaaaa tgaccgctga
      841 agcccgccag attcatccag aaggtgtgtt gattgatggc gaattggtat accatgagca
      901 agttaaaaag gagccagaag gcctagattt tctttttgat gcttatcctg aaaacagtaa
      961 agctaaagaa ttcgccgaag ttgctgaatc acgtactgct tctaatggaa tcgccaataa
     1021 atctttaaag ggaaccattt ctgaaaaaga agcacaatgc atgaagtttc aggtctggga
     1081 ttatgtcccg ttggtagaaa tatacagtct tcctgcattt cgtttgaaat atgatgtacg
     1141 tttttctaaa ctagaacaaa tgacatctgg atatgataaa gtaattttaa ttgaaaacca
     1201 ggtagtaaat aacctagatg aagctaaggt aatttataaa aagtatattg accaaggtct
     1261 tgaaggtatt attctcaaaa atatcgatgg attatgggaa aatgctcgtt caaaaaatct
     1321 ttataaattt aaagaagtaa ttgatgttga tttaaaaatt gtaggaattt atcctcaccg
     1381 taaagaccct actaaagcgg gtggatttat tcttgagtca gagtgtggaa aaattaaggt
     1441 aaatgctggt tcaggcttaa aagataaagc cggtgtaaaa tcgcatgaac ttgaccgtac
     1501 tcgcattatg gaaaaccaaa attattatat tggaaaaatt ctagagtgcg aatgcaacgg
     1561 ttggttaaaa tctgatggcc gcactgatta cgttaaatta tttcttccga ttgcgattcg
     1621 tttacgtgaa gataaaacta aagctaatac attcgaagat gtatttggtg attttcatga
     1681 ggtaactggt ctataaggcc tatgcggccg ctaagggtcg acctgcagcc aagcttaatt
     1741 agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     1801 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctaggggaa
     1861 ttcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     1921 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     1981 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     2041 cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg atacgcctat
     2101 ttttataggt taatgtcatg ataataatgg tttcttagaa tcctgatgtc cggcggtgct
     2161 tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga
     2221 agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac
     2281 ccgacgcact ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
     2341 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
     2401 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     2461 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     2521 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     2581 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     2641 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     2701 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     2761 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     2821 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2881 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     2941 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     3001 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     3061 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
     3121 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     3181 attggtgccc ttaaacgcct ggtgctacgc ctgaataagt gataataagc ggatgaatgg
     3241 cagaaattcg aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc
     3301 gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc
     3361 ttctcaaatg cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat
     3421 atgatcattt attctgcctc ccagagcctg ataaaaacgg ttaggatcgg agtcaggcaa
     3481 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3541 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3601 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3661 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3721 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3781 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3841 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3901 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3961 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4021 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4081 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4141 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4201 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4261 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4321 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4381 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4441 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     4501 ttctccttac gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggt
     4561 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
     4621 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     4681 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     4741 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     4801 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     4861 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     4921 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
     4981 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     5041 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     5101 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     5161 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
     5221 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     5281 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     5341 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     5401 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     5461 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     5521 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     5581 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
     5641 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5701 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     5761 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     5821 aacagctatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga
     5881 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5941 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgc
//
