LOCUS Exported 6200 bp ds-DNA circular SYN 08-NOV-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCA24N-sso7d-ligase SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6200) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, Jan 31, 2017 from SnapGene Viewer 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6200 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 71..163 /note="T5-lac promoter" CDS 185..235 /codon_start=1 /note="His-tag" /translation="MRGSHHHHHHTDPALRA" CDS 236..421 /codon_start=1 /note="sso7d" /translation="TVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVS EKDAPKELLQMLEKQKK" CDS 422..451 /codon_start=1 /note="Linker" /translation="GTSGGGSGGG" CDS 452..1912 /codon_start=1 /note="T4 ligase gene" /translation="ILKILNEIASIGSTKQKQAILEKNKDNELLKRVYCLTYSRGLQYY IKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVL RRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGAR CFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQ VKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVW DYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQ GLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGK IKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLP IAIRLREDKTKANTFEDVFGDFHEVTGL" CDS 1913..1930 /codon_start=1 /note="Ext. (ex-GFP)" /translation="GLCGR" terminator 2099..2280 /note="rrnB terminator" CDS 2721..3380 /codon_start=1 /note="CAT gene" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" rep_origin 4516 /direction=RIGHT /note="ori" CDS 4839..5921 /codon_start=1 /note="LacIq" /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" ORIGIN 1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat 121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt 181 aactatgaga ggatctcacc atcaccatca ccatacggat ccggccctga gggcgactgt 241 taaattcaaa tacaaaggtg aggagaaaga agttgacatt tccaagatca aaaaggtatg 301 gcgcgtgggc aaaatgatct ctttcactta cgacgaaggc ggcggtaaaa ccggtcgtgg 361 cgcggtgagc gaaaaggacg cgccaaaaga actgctgcag atgctggaaa aacagaagaa 421 aggcactagt ggcggaggct ccggcggtgg cattcttaaa attctgaacg aaatagcatc 481 tattggttca actaaacaga agcaagcaat tcttgaaaag aataaagata atgaattgct 541 taaacgagta tattgtctga cttattctcg tgggttacag tattatatca agaaatggcc 601 taaacctggt attgctaccc agagttttgg aatgttgact cttaccgata tgcttgactt 661 cattgaattc acattagcta ctcggaaatt gactggaaat gcagcaattg aggaattaac 721 tggatatatc accgatggta aaaaagatga tgttgaagtt ttgcgtcgag tgatgatgcg 781 agaccttgaa tgtggtgctt cagtatctat tgcaaacaaa gtttggccag gtttaattcc 841 tgaacaacct caaatgctcg caagttctta tgatgaaaaa ggcattaata agaatatcaa 901 atttccagcc tttgctcagt taaaagctga tggagctcgg tgttttgctg aagttagagg 961 tgatgaatta gatgatgttc gtcttttatc acgagctggt aatgaatatc taggattaga 1021 tcttcttaag gaagagttaa ttaaaatgac cgctgaagcc cgccagattc atccagaagg 1081 tgtgttgatt gatggcgaat tggtatacca tgagcaagtt aaaaaggagc cagaaggcct 1141 agattttctt tttgatgctt atcctgaaaa cagtaaagct aaagaattcg ccgaagttgc 1201 tgaatcacgt actgcttcta atggaatcgc caataaatct ttaaagggaa ccatttctga 1261 aaaagaagca caatgcatga agtttcaggt ctgggattat gtcccgttgg tagaaatata 1321 cagtcttcct gcatttcgtt tgaaatatga tgtacgtttt tctaaactag aacaaatgac 1381 atctggatat gataaagtaa ttttaattga aaaccaggta gtaaataacc tagatgaagc 1441 taaggtaatt tataaaaagt atattgacca aggtcttgaa ggtattattc tcaaaaatat 1501 cgatggatta tgggaaaatg ctcgttcaaa aaatctttat aaatttaaag aagtaattga 1561 tgttgattta aaaattgtag gaatttatcc tcaccgtaaa gaccctacta aagcgggtgg 1621 atttattctt gagtcagagt gtggaaaaat taaggtaaat gctggttcag gcttaaaaga 1681 taaagccggt gtaaaatcgc atgaacttga ccgtactcgc attatggaaa accaaaatta 1741 ttatattgga aaaattctag agtgcgaatg caacggttgg ttaaaatctg atggccgcac 1801 tgattacgtt aaattatttc ttccgattgc gattcgttta cgtgaagata aaactaaagc 1861 taatacattc gaagatgtat ttggtgattt tcatgaggta actggtctat aaggcctatg 1921 cggccgctaa gggtcgacct gcagccaagc ttaattagct gagcttggac tcctgttgat 1981 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg 2041 ggcgtttttt attggtgaga atccaagcta ggggaattca tcgtgactga ctgacgatct 2101 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 2161 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 2221 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 2281 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 2341 taatggtttc ttagaatcct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc 2401 agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca 2461 ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa 2521 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg 2581 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca 2641 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt 2701 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 2761 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 2821 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 2881 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 2941 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 3001 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 3061 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 3121 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 3181 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 3241 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 3301 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 3361 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg 3421 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac 3481 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc 3541 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt 3601 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag 3661 agcctgataa aaacggttag gatcggagtc aggcaactat ggatgaacga aatagacaga 3721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 3781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 4021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 4081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 4141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 4201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 4621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 4681 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 4741 tttcacaccg cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat 4801 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg 4861 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca 4921 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca 4981 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca 5041 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg 5101 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta 5161 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc 5221 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc 5281 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc 5341 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc 5401 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca 5461 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac 5521 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc 5581 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata 5641 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca 5701 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct 5761 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa 5821 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 5881 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 5941 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 6001 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga 6061 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 6121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 6181 tcgcccttcc caacagttgc //