LOCUS       Exported                6200 bp ds-DNA     circular SYN 08-NOV-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCA24N-sso7d-ligase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 31, 2017 from SnapGene Viewer 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        71..163
                     /note="T5-lac promoter"
     CDS             185..235
                     /codon_start=1
                     /note="His-tag"
                     /translation="MRGSHHHHHHTDPALRA"
     CDS             236..421
                     /codon_start=1
                     /note="sso7d"
                     /translation="TVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVS
                     EKDAPKELLQMLEKQKK"
     CDS             422..451
                     /codon_start=1
                     /note="Linker"
                     /translation="GTSGGGSGGG"
     CDS             452..1912
                     /codon_start=1
                     /note="T4 ligase gene"
                     /translation="ILKILNEIASIGSTKQKQAILEKNKDNELLKRVYCLTYSRGLQYY
                     IKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVL
                     RRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGAR
                     CFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQ
                     VKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVW
                     DYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQ
                     GLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGK
                     IKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLP
                     IAIRLREDKTKANTFEDVFGDFHEVTGL"
     CDS             1913..1930
                     /codon_start=1
                     /note="Ext. (ex-GFP)"
                     /translation="GLCGR"
     terminator      2099..2280
                     /note="rrnB terminator"
     CDS             2721..3380
                     /codon_start=1
                     /note="CAT gene"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      4516
                     /direction=RIGHT
                     /note="ori"
     CDS             4839..5921
                     /codon_start=1
                     /note="LacIq"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
       61 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat
      121 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt
      181 aactatgaga ggatctcacc atcaccatca ccatacggat ccggccctga gggcgactgt
      241 taaattcaaa tacaaaggtg aggagaaaga agttgacatt tccaagatca aaaaggtatg
      301 gcgcgtgggc aaaatgatct ctttcactta cgacgaaggc ggcggtaaaa ccggtcgtgg
      361 cgcggtgagc gaaaaggacg cgccaaaaga actgctgcag atgctggaaa aacagaagaa
      421 aggcactagt ggcggaggct ccggcggtgg cattcttaaa attctgaacg aaatagcatc
      481 tattggttca actaaacaga agcaagcaat tcttgaaaag aataaagata atgaattgct
      541 taaacgagta tattgtctga cttattctcg tgggttacag tattatatca agaaatggcc
      601 taaacctggt attgctaccc agagttttgg aatgttgact cttaccgata tgcttgactt
      661 cattgaattc acattagcta ctcggaaatt gactggaaat gcagcaattg aggaattaac
      721 tggatatatc accgatggta aaaaagatga tgttgaagtt ttgcgtcgag tgatgatgcg
      781 agaccttgaa tgtggtgctt cagtatctat tgcaaacaaa gtttggccag gtttaattcc
      841 tgaacaacct caaatgctcg caagttctta tgatgaaaaa ggcattaata agaatatcaa
      901 atttccagcc tttgctcagt taaaagctga tggagctcgg tgttttgctg aagttagagg
      961 tgatgaatta gatgatgttc gtcttttatc acgagctggt aatgaatatc taggattaga
     1021 tcttcttaag gaagagttaa ttaaaatgac cgctgaagcc cgccagattc atccagaagg
     1081 tgtgttgatt gatggcgaat tggtatacca tgagcaagtt aaaaaggagc cagaaggcct
     1141 agattttctt tttgatgctt atcctgaaaa cagtaaagct aaagaattcg ccgaagttgc
     1201 tgaatcacgt actgcttcta atggaatcgc caataaatct ttaaagggaa ccatttctga
     1261 aaaagaagca caatgcatga agtttcaggt ctgggattat gtcccgttgg tagaaatata
     1321 cagtcttcct gcatttcgtt tgaaatatga tgtacgtttt tctaaactag aacaaatgac
     1381 atctggatat gataaagtaa ttttaattga aaaccaggta gtaaataacc tagatgaagc
     1441 taaggtaatt tataaaaagt atattgacca aggtcttgaa ggtattattc tcaaaaatat
     1501 cgatggatta tgggaaaatg ctcgttcaaa aaatctttat aaatttaaag aagtaattga
     1561 tgttgattta aaaattgtag gaatttatcc tcaccgtaaa gaccctacta aagcgggtgg
     1621 atttattctt gagtcagagt gtggaaaaat taaggtaaat gctggttcag gcttaaaaga
     1681 taaagccggt gtaaaatcgc atgaacttga ccgtactcgc attatggaaa accaaaatta
     1741 ttatattgga aaaattctag agtgcgaatg caacggttgg ttaaaatctg atggccgcac
     1801 tgattacgtt aaattatttc ttccgattgc gattcgttta cgtgaagata aaactaaagc
     1861 taatacattc gaagatgtat ttggtgattt tcatgaggta actggtctat aaggcctatg
     1921 cggccgctaa gggtcgacct gcagccaagc ttaattagct gagcttggac tcctgttgat
     1981 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
     2041 ggcgtttttt attggtgaga atccaagcta ggggaattca tcgtgactga ctgacgatct
     2101 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     2161 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     2221 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     2281 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2341 taatggtttc ttagaatcct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc
     2401 agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca
     2461 ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa
     2521 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg
     2581 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca
     2641 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     2701 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     2761 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     2821 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     2881 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     2941 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     3001 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     3061 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     3121 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     3181 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     3241 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     3301 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     3361 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg
     3421 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     3481 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     3541 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     3601 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag
     3661 agcctgataa aaacggttag gatcggagtc aggcaactat ggatgaacga aatagacaga
     3721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4681 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4741 tttcacaccg cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat
     4801 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
     4861 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     4921 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     4981 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     5041 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     5101 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     5161 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     5221 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     5281 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     5341 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     5401 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     5461 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     5521 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     5581 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     5641 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     5701 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     5761 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     5821 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5881 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5941 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6001 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga
     6061 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6181 tcgcccttcc caacagttgc
//
