LOCUS       Exported               10306 bp ds-DNA     circular UNA 16-MAR-2017
DEFINITION  Gibson Assembly of Chabot pKaiBC-yfp (corrected by sequencing) 
            extraction into EB2065(XbaI,XhoI).
ACCESSION   urn.local...4f-47sn4lu
VERSION     .
KEYWORDS    EB2316
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Addgene
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 16, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          3908..4840
                     /organism="Synechococcus elongatus PCC 7942"
                     /strain="PCC 7942"
                     /mol_type="genomic DNA"
                     /label=source Synechococcus elongatus PCC 7942
                     /note="source Synechococcus elongatus PCC 7942"
                     /db_xref="taxon:1140"
     source          9208..9263
                     /organism="Synechococcus elongatus PCC 7942"
                     /strain="PCC 7942"
                     /mol_type="genomic DNA"
                     /label=source Synechococcus elongatus PCC 7942
                     /note="source Synechococcus elongatus PCC 7942"
                     /db_xref="taxon:1140"
     source          9264..10274
                     /organism="Synechococcus elongatus PCC 7942"
                     /strain="PCC 7942"
                     /mol_type="genomic DNA"
                     /label=source Synechococcus elongatus PCC 7942
                     /note="source Synechococcus elongatus PCC 7942"
                     /db_xref="taxon:1140"
     misc_feature    1..1716
                     /label=ChabotpKaiBC-yfp
                     /note="ChabotpKaiBC-yfp"
                     /note="Geneious type: Inserted sequence modified_by=User"
     primer_bind     1..16
                     /label=Chabot pKaiBC-yfp (corrected by sequencing) 
                     extraction (fwd) - EB2065(XbaI,XhoI)
                     /note="Chabot pKaiBC-yfp (corrected by sequencing) ext"
                     /note="Hairpin_Tm=45.7 Product_Size=1716 %GC=43.8 
                     Pair_Dimer_Tm=None Sequence=CTAGACGCAAATTGCA 
                     annotation_group=14217850087994890000423910f-f718-4a7a-a 
                     633-9829fd7cdac3Chabot pKaiBC-yfp (corrected by sequencing)
                     extraction Tm=61.3 Self_Dimer_Tm=18.7 
                     Extension=GATATCGTCGACAGGCCT created_by=primer3"
     promoter        6..942
                     /label=Chabot pKaiBC
                     /note="Chabot pKaiBC created_by=User"
                     /note="created_by=User"
     CDS             964..1680
                     /label=YFP
                     /note="YFP"
                     /note="created_by=User"
     CDS             1681..1716
                     /label=LVA
                     /note="LVA"
                     /note="created_by=User"
     misc_feature    complement(1697..1716)
                     /label=Chabot pKaiBC-yfp (corrected by sequencing) 
                     extraction (rev) - EB2065(XbaI,XhoI)
                     /note="Chabot pKaiBC-yfp (corrected by sequencing) ext"
                     /note="Geneious type: primer_bind_reverse Hairpin_Tm=56.5 
                     Product_Size=1716 %GC=35.0 Pair_Dimer_Tm=None 
                     Sequence=ATTAAGCTACTAAAGCGTAG 
                     annotation_group=14217850087994890000423910f-f718-4a7a-a 
                     633-9829fd7cdac3Chabot pKaiBC-yfp (corrected by sequencing)
                     extraction Tm=59.0 Self_Dimer_Tm=None 
                     Extension=CCGGCCAAGCTTGCTAGC created_by=primer3"
     terminator      complement(1767..1794)
                     /label=rrnB_T2 term
                     /note="rrnB_T2 term Transferred_From=rrnB_T2 term"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=rrnB_T2 term 
                     Transferred_Similarity=100.00%"
     terminator      complement(1926..1969)
                     /label=rrnB_T1 term
                     /note="rrnB_T1 term Transferred_From=rrnB_T1 term"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=rrnB_T1 term 
                     Transferred_Similarity=97.73%"
     terminator      complement(1935..2092)
                     /label=rrnB term
                     /note="rrnB term Transferred_From=rrnB term"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=rrnB term 
                     Transferred_Similarity=100.00%"
     promoter        complement(2591..2619)
                     /label=amp prom
                     /note="amp prom Transferred_From=amp prom"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=amp prom 
                     Transferred_Similarity=100.00%"
     misc_feature    2960..3619
                     /label=CAT marker
                     /label=marker
                     /note="CAT marker"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=CAT marker 
                     Transferred_Similarity=99.85%"
     primer_bind     complement(3890..3907)
                     /label=EB2065 R
                     /note="EB2065 R"
                     /note="Hairpin_Tm=63.6 modified_by=User %GC=72.2 
                     Sequence=CCGCGCTGGGGTTACCAG Tm=63.1 Self_Dimer_Tm=10.0"
     misc_feature    3908..4840
                     /label=NS2.2
                     /note="NS2.2"
                     /note="created_by=User"
     gene            complement(3908..4840)
                     /locus_tag="Synpcc7942_0086"
                     /label=gene
                     /note="Synpcc7942_0086"
                     /db_xref="GeneID:3773426"
     CDS             complement(3908..4840)
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="Synpcc7942_0086"
                     /product="isoamylase"
                     /EC_number="3.2.1.68"
                     /label=isoamylase CDS
                     /note="Synpcc7942_0086"
                     /db_xref="GI:81298897"
                     /db_xref="InterPro:IPR006589"
                     /db_xref="InterPro:IPR011837"
                     /db_xref="GeneID:3773426"
                     /inference="non-experimental evidence, no additional 
                     details recorded"
                     /protein_id="YP_399105.1"
                     /translation="MSPSTHTLRSHSNL*AACWRLHQASQFRRRP*KTWHLRWSNRKNS
                     LPAIPRRHGR*VAAGAPVRSPRCPLRTRELLGLQHHGFFCAPRSLQLSP*STWSS**VP
                     RPRQGAPPSRD*GDSRRGVQPHC*RE*RRSNAVFQRSSEFNLLSAG*TGGLSQLHRLRQ
                     HRQS*QFDRAIADSRLPALLGLGNARRWLPL*PCVGAES*CQWQPPIGSALALGD*FRS
                     GFGRYEAHC*SLGRSRLISGWYLYWRSLWDLERSLPGRYSAFLAWRSGLYLRPQSTPAG
                     *PRCLQHRPMVCRTHH*LHHLP*RLYAARS"
     misc_feature    3908..3913
                     /label=BglII
                     /note="BglII"
                     /note="Geneious type: restriction site 
                     Recognition_pattern=A^GATCT annotation_group=AGATCT"
     misc_feature    4108..4113
                     /label=KpnI
                     /note="KpnI"
                     /note="Geneious type: restriction site 
                     Recognition_pattern=GGTAC^C annotation_group=GGTACC"
     misc_feature    complement(4841..5741)
                     /label=tet marker
                     /label=marker
                     /note="tet marker"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=tet marker 
                     Transferred_Similarity=99.58%"
     misc_feature    4841..4844
                     /label=BamHI overhang
                     /label=overhang
                     /note="BamHI overhang"
     misc_feature    5027..5032
                     /label=SphI
                     /note="SphI"
                     /note="Geneious type: restriction site 
                     Recognition_pattern=GCATG^C annotation_group=GCATGC"
     misc_feature    5437..5442
                     /label=NruI
                     /note="NruI"
                     /note="Geneious type: restriction site 
                     Recognition_pattern=TCG^CGA annotation_group=TCGCGA"
     gene            6380..6571
                     /label=ROP
                     /note="ROP"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=ROP 
                     Transferred_Similarity=100.00%"
     misc_feature    6760..6765
                     /label=NdeI
                     /note="NdeI"
                     /note="Geneious type: restriction site 
                     Recognition_pattern=CA^TATG annotation_group=CATATG"
     misc_feature    complement(6984..7603)
                     /label=pBR322 origin
                     /note="pBR322 origin"
                     /note="Geneious type: origin of replication 
                     Imported_from=Pla'>http://wishart.biology.ualberta.ca/PlasM
                      apper"">Pla sMapper Transferred_From=pBR322 origin 
                     Transferred_Similarity=99.68%"
     misc_feature    complement(7758..8618)
                     /label=amp marker
                     /label=marker
                     /note="amp marker"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=amp marker 
                     Transferred_Similarity=99.88%"
     promoter        complement(8660..8688)
                     /label=amp prom
                     /note="amp prom Transferred_From=amp prom"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=amp prom 
                     Transferred_Similarity=100.00%"
     misc_feature    8849..8854
                     /label=ClaI
                     /note="ClaI"
                     /note="Geneious type: restriction site 
                     Recognition_pattern=AT^CGAT annotation_group=ATCGAT"
     misc_feature    complement(8912..9201)
                     /label=tet marker
                     /label=marker
                     /note="tet marker"
                     /note="Imported_from=Pla'>http://wishart.biology.ualberta.c
                      a/PlasMapper"">Pla sMapper Transferred_From=tet marker 
                     Transferred_Similarity=99.58%"
     misc_feature    9201..9202
                     /label=origin
                     /label=origin
                     /note="origin"
     misc_feature    complement(9208..9263)
                     /label=NS2.1
                     /note="NS2.1"
                     /note="modified_by=User created_by=User"
     gene            complement(9208..9263)
                     /locus_tag="Synpcc7942_0085"
                     /label=gene
                     /note="Synpcc7942_0085"
                     /db_xref="GeneID:3773425"
     CDS             complement(9208..9263)
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="Synpcc7942_0085"
                     /product="HAD family hydrolase"
                     /label=HAD family hydrolase CDS
                     /note="Synpcc7942_0085"
                     /db_xref="GI:81298896"
                     /db_xref="InterPro:IPR006402"
                     /db_xref="GeneID:3773425"
                     /inference="non-experimental evidence, no additional 
                     details recorded"
                     /protein_id="YP_399104.1"
                     /translation="MYCVASSGDYQKMRTTLS"
     misc_feature    9264..10274
                     /label=NS2.2-
                     /note="NS2.2-"
                     /note="created_by=User"
     gene            complement(9264..9572)
                     /locus_tag="Synpcc7942_0085"
                     /label=gene
                     /note="Synpcc7942_0085"
                     /db_xref="GeneID:3773425"
     CDS             complement(9264..9572)
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="Synpcc7942_0085"
                     /product="HAD family hydrolase"
                     /label=HAD family hydrolase CDS
                     /note="Synpcc7942_0085"
                     /db_xref="GI:81298896"
                     /db_xref="InterPro:IPR006402"
                     /db_xref="GeneID:3773425"
                     /inference="non-experimental evidence, no additional 
                     details recorded"
                     /protein_id="YP_399104.1"
                     /translation="MQTSPFQLVIFDCDGVLVDSERITNRVFADMLNELGLLVTLDDMF
                     EQFVGHSMADCLKLIERRLGNPPPPDFVQHYQRRTRIALETHLQAVPGVEEALDALEL"
     misc_feature    complement(9264..9512)
                     /label=NS2.1
                     /note="NS2.1"
                     /note="modified_by=User created_by=User"
     gene            complement(9578..10274)
                     /locus_tag="Synpcc7942_0086"
                     /label=gene
                     /note="Synpcc7942_0086"
                     /db_xref="GeneID:3773426"
     CDS             complement(9578..10274)
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="Synpcc7942_0086"
                     /product="isoamylase"
                     /EC_number="3.2.1.68"
                     /label=isoamylase CDS
                     /note="Synpcc7942_0086"
                     /db_xref="GI:81298897"
                     /db_xref="InterPro:IPR006589"
                     /db_xref="InterPro:IPR011837"
                     /db_xref="GeneID:3773426"
                     /inference="non-experimental evidence, no additional 
                     details recorded"
                     /protein_id="YP_399105.1"
                     /translation="MQL*PEAQLCQWREQSGRDQ*QLQLELRH*RRDR*PHDSELTGTA
                     AAQFARHVIPRPGHTDADDGR*GQTQSAG*Q*RLLPRQ*DQLV*LVAVRSPCRFLGVQS
                     PPD*TFPVAGDVPTERTAAERTPSASSLCHLAWRQTQTTRLGAVVPQSGRQSLPSSPAG
                     MALPSL*CLLGRPALPVAEASSRPRLVSLARYFTAES*SLSSAG*GKTLPTARLHRPSA
                     IALTVDGSCL"
     misc_feature    9713..9720
                     /label=NotI
                     /note="NotI"
                     /note="Geneious type: restriction site 
                     Recognition_pattern=GC^GGCCGC annotation_group=GCGGCCGC"
     primer_bind     10275..10290
                     /label=EB2065 F
                     /note="EB2065 F"
                     /note="Hairpin_Tm=85.5 %GC=62.5 Sequence=CGTCACGGGTAACCGA 
                     Tm=55.6 Self_Dimer_Tm=29.5 created_by=primer3"
ORIGIN
        1 ctagacgcaa attgcaatct gtatttgggt ggaatcgacg gcaattttgc aggattgcca
       61 gcgggcgctg tcggccgatc gctatcaact ccaagtctgt gagtctggcg aaatgctctt
      121 ggagtatgcc caaacccatc gtgaccaaat cgactgcctg attttagtgg cagccaatcc
      181 cagcttcagg gcagttgttc agcagctctg ctttgaggga gtggtggtac cagcgattgt
      241 cgtaggcgat cgcgacagtg aggatcccga tgaaccagcc aaagaacagc tctatcacag
      301 cgctgaactg cacctcggta tccatcagct cgagcaattg ccctaccaag ttgatgctgc
      361 actggctgaa tttctgcgct tagccccggt cgagaccatg gccgaccaca tcatgctgat
      421 gggggccaac cacgatcccg agctatcgag ccagcagcgg gacctcgctc agcgactaca
      481 agagcgccta ggctatctcg gggtctacta caagcgtgat cccgatcgct ttctgcgcaa
      541 cctacccgcc tacgaaagcc aaaagctgca ccaagcgatg cagactagct atcgtgaaat
      601 cgttttgagc tatttttcgc cgaatagcaa cctcaaccag agcattgaca acttcgtcaa
      661 catggctttc tttgccgatg ttccagtcac caaagtggta gaaattcaca tggagctgat
      721 ggacgagttt gccaagaagc tccgcgtaga gggacgttca gaggacattt tgctggatta
      781 tcggctgact ttaattgatg taattgcaca tctttgtgag atgtatcgac ggtctatccc
      841 acgagaaacc tgaaaaggta aaggaggtct taagctcggc tcaatttctc tctttatcct
      901 gttagatggt ttgattgctg ttgctacccc gttgatctgc gtagatccta aggaggaaaa
      961 aaaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1021 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     1081 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1141 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     1201 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1261 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaggttcga gggcgacacc
     1321 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     1381 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     1441 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     1501 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1561 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1621 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1681 gcacaaacga cgaaaactac gctttagtag cttaatgcta gcaagcttgg ccggatccgg
     1741 ccggatccgg agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa
     1801 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg
     1861 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     1921 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg
     1981 cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg gcgtttcact
     2041 tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg caaattctgt
     2101 tttatcagcc gttaccccac ctactagcta atcccatctg ggcacatccg atggcaagag
     2161 gcccgaaggt ccccctcttt ggtcttgcga cgttatgcgg tattagctac cgtttccagt
     2221 agttatcccc ctccatcagg cagtttccca gacattactc acccgtccgc cactcgtcag
     2281 caaagaagca agcttagatc cgtcacacgg gataataccg cgccacatag cagaacttta
     2341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2701 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcgaataaat
     2761 acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg
     2821 gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
     2881 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
     2941 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     3001 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     3061 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     3121 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     3181 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     3241 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     3301 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     3361 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     3421 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     3481 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     3541 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     3601 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     3661 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     3721 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     3781 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     3841 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcgacggatc tggtaacccc
     3901 agcgcggaga tctcgcagcg taaagccgtc atggcaggtg atgaagttaa tggtgcgtcc
     3961 ggcataccat tggtctgtgc tgtagacatc ggggctaccc agcaggcgtt gactgagggc
     4021 gtaagtacag ccctgatctc cacgccaaaa acgccgaata tcgtcccgga agggaccgtt
     4081 ccaagtccca aagcgatcgc caataaaggt accaacctga tataagccgg ctgcgtccca
     4141 agcttcagca atgagcttcg taccggccaa aaccggatcg gaatcaatcg cccaaagcaa
     4201 gggcggatcc gatagggggt tgccattggc atcacgactc agcaccgacg caaggtcaaa
     4261 gcggaagcca tcgacgtgca tttccgagac ccaataacgc aggcaatcga gaatcagcga
     4321 tcgcacgatc gaattgttag ctttgacggt gttgccgcag ccggtgtagt tgcgatagcc
     4381 cgcctgttca tccagcagat agtaggttga attcgctaga cctttgaaag acagcgttgg
     4441 accgtcttca ttcccttcag cagtgtggtt gaacaccacg tcgagaatca cctcaatccc
     4501 tgcttggtgg agcgccttga cgaggtcgcg gaactcatca actggaccaa gtggatcatg
     4561 gcgagagctg taggctgcgt ggggcgcaaa aaaagccatg gtgctgtagc cccagtagtt
     4621 ctcgcgtcct aagggggcat cttggcgatc gaactggtgc accggcagca actcaacggc
     4681 cgtgacgccg agggattgca ggtagggaat tttttcgatt agaccagcgt aggtgccacg
     4741 tttttcaggg gcgacgccgg aattgggatg cttggtgaag ccgccaacat gcagctcata
     4801 gattactgtg cgagcgtagg gtgtgcgtgg atggcgatcg gatcctctac gccggacgca
     4861 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     4921 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     4981 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     5041 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     5101 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     5161 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     5221 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     5281 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     5341 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     5401 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     5461 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     5521 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     5581 ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
     5641 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
     5701 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     5761 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
     5821 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
     5881 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     5941 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     6001 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     6061 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     6121 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     6181 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
     6241 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
     6301 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
     6361 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
     6421 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
     6481 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc
     6541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     6601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     6661 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     6721 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     6781 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     6841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8041 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     8101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8341 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     8401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8761 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     8821 caagaattct catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca
     8881 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
     8941 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
     9001 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
     9061 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
     9121 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
     9181 ggcgaccaca cccgtcctgt ggatcccagg ctcagtgtgg ttcgcatctt ttgataatca
     9241 ccactggacg caacacagta gggcaattca agagcatcca aagcctcttc aaccccagga
     9301 acggcttgta gatgcgtttc taacgcgata cgggtacggc gttgatagtg ctgaacaaag
     9361 tcagggggtg gaggattgcc taaccgtcgc tcaattagtt tgagacagtc agccatggaa
     9421 tgacccacaa actgctcaaa catgtcatcc aaagtcacca acagacccag ttcattgagc
     9481 atgtctgcaa agacgcgatt agtgatgcgt tcgctatcaa caagcacacc atcacagtcg
     9541 aaaatcacca gctgaaacgg tgaagtttgc attgttttta agcacgagcc atcaacagta
     9601 agagcgatcg cgctgggacg atgtaatcgc gccgtaggca gggttttgcc tcatccggca
     9661 gatgacaagc ttcaagattc ggcagtgaag tatcgagcaa gcgataccaa acgcggccgc
     9721 gaggaggcct cggcaactgg aagcgcaggt cttcccagta agcattaaag gctaggtaaa
     9781 gccattcctg ctggcgagga tggcagagac tgacggccag actgtgggac cacagcgccc
     9841 aatcgggttg tttgagtttg acgccatgcc agatggcata gggacgacgc ggatggggtt
     9901 cgttctgcag cagttcgttc tgttggaaca tcaccagcga ctgggaaagt tcaatcaggc
     9961 ggcgactgaa caccaagaaa tcggcatggc gatcgcacag cgaccaatca aaccagctga
    10021 tctcattgtc ttggcagtag gcgttattgt taccctgctg actgcgtttg acctcatcgc
    10081 ccatcgtcag catcggtgtg ccctgggcga ggaataacgt ggcgagcaaa ttgcgctgct
    10141 gccgttcccg taagctcaga atcgtggggt catcggtctc gccttcaatg ccgtagttcc
    10201 agctgtagtt gtcattggtc ccgtcccgat tgttctctcc attggcaaag ttgtgcttct
    10261 ggctatagct gactcgtcac gggtaaccga tatcgtcgac aggcct
//
