LOCUS       tetR:linker_-_mT        9349 bp    DNA     circular UNA 31-JAN-2017
DEFINITION  Gibson Assembly of tetR:linker - mTurq2 into AM2991(BamHI,EcoRI)
            (reversed).
ACCESSION   urn.local...2d-426v3kf
VERSION     urn.local...2d-426v3kf
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    17..22
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     rRNA            53..478
                     /vntifkey=58
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="rrnB"
     terminator      258..301
                     /vntifkey=43
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="terminator\T1"
     terminator      433..460
                     /vntifkey=43
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="terminator\T2"
     misc_feature    532..539
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     terminator      597..666
                     /vntifkey=43
                     /ApEinfo_label="T4\terminator"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="T4\terminator(1)"
     CDS             complement(926..1936)
                     /vntifkey=4
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="SpR"
     CDS             complement(1958..2264)
                     /vntifkey=4
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="ORF3-2"
     terminator      complement(2453..2522)
                     /vntifkey=43
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="T4\terminator"
     misc_recomb     2591..3507
                     /vntifkey=86
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="NSI"
     old_sequence    complement(3493..5272)
                     /vntifkey=89
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="pBR322\sequences"
     misc_feature    3721..3726
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI"
                     /note="Geneious type: restriction site"
     origin          5272^5273
                     /label="origin"
     misc_recomb     5445..6311
                     /vntifkey=86
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="-NSI"
     CDS             6591..7682
                     /vntifkey=4
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="lacIq"
     misc_feature    7456
                     /created_by="User"
                     /label="mutation7461C2T"
     misc_feature    7701
                     /created_by="User"
                     /modified_by="User"
                     /label="mutation7707G2A"
     promoter        7739..7984
                     /vntifkey=30
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="Ptrc"
     misc_binding    7948..7969
                     /vntifkey=20
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /label="lacO"
     misc_feature    7992..7993
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    7994..9349
                     /modified_by="User"
                     /label="tetR-linker-mTurq2"
                     /note="Geneious type: Inserted sequence"
     primer_bind     7994..8018
                     /created_by="primer3"
                     /%GC="24.0"
                     /Tm="59.4"
                     /Hairpin_Tm="37.1"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="ATGTCTAGATTAGATAAAAGTAAAG"
                     /Product_Size=636
                     /annotation_group="1420664484727951000607ec3f3-888a-4f8b-9
                     50c-294e45e5a023tetR:linker"
                     /Extension="ACAGGAAACAGACCATGGAA"
                     /label="tetR:linker (fwd) - AM2991(BamHI,EcoRI)
                     (reversed)"
     CDS             7994..8614
                     /created_by="User"
                     /label="tetR"
     misc_feature    complement(8613..8629)
                     /created_by="primer3"
                     /%GC="52.9"
                     /Tm="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="14.3"
                     /Pair_Dimer_Tm="None"
                     /Sequence="CAACTCGAGGATGTCAG"
                     /Product_Size=636
                     /annotation_group="1420664484727951000607ec3f3-888a-4f8b-9
                     50c-294e45e5a023tetR:linker"
                     /Extension="CGAAACCAT"
                     /label="tetR:linker (rev) - mTurq2"
                     /note="Geneious type: primer_bind_reverse"
     motif           8615..8629
                     /created_by="User"
                     /label="Linker"
     gene            8630..9349
                     /created_by="User"
                     /label="mTurq2"
     primer_bind     8630..8643
                     /created_by="primer3"
                     /%GC="50.0"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="ATGGTTTCGAAGGG"
                     /Product_Size=720
                     /annotation_group="14206644853221840005434f91f-0f0b-472d-a
                     bc7-f3bafafa400emTurq2"
                     /Extension="CTCGAGTTG"
                     /label="mTurq2 (fwd) - tetR:linker"
     misc_feature    complement(9328..9349)
                     /created_by="primer3"
                     /%GC="31.8"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Pair_Dimer_Tm="None"
                     /Sequence="CTACTTATACAATTCATCCATC"
                     /Product_Size=720
                     /annotation_group="14206644853221840005434f91f-0f0b-472d-a
                     bc7-f3bafafa400emTurq2"
                     /Extension="CCCCGGGTACCGAGCTCG"
                     /label="mTurq2 (rev) - AM2991(BamHI,EcoRI) (reversed)"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt
       61 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
      121 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
      181 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
      241 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      301 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
      361 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
      421 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
      481 ttgtttattt ttctaaatac attcaaatat gtatccgctc atggcggcca cgcggccgcc
      541 gatcctctag agctttatgc ttgtaaaccg ttttgtgaaa aaatttttaa aataaaaaag
      601 gggacctcta gggtccccaa ttaattagta atataatcta ttaaaggtca ttcaaaaggt
      661 catccaccgg atcagcttag taaagccctc gctagatttt aatgcggatg ttgcgattac
      721 ttcgccaact attgcgataa caagaaaaag ccagcctttc atgatatatc tcccaatttg
      781 tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc
      841 tgtgagcaat tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc
      901 gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca
      961 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     1021 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     1081 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     1141 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
     1201 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     1261 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     1321 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     1381 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     1441 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     1501 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     1561 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     1621 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     1681 gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc
     1741 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
     1801 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac
     1861 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     1921 agttgcgtga gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc
     1981 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
     2041 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
     2101 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
     2161 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
     2221 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagc
     2281 ttctgtatgg aacgggcatg cggatcagtg agggtttgca actgcgggtc aaggatctgg
     2341 atttcgatca cggcacgatc atcgtgcggg agggcaaggg ctccaaggat cgggccttga
     2401 tgttacccga gagcttggca cccagcctgc gcgagcaggg gaattgatcc ggtggatgac
     2461 cttttgaatg acctttaata gattatatta ctaattaatt ggggacccta gaggtcccct
     2521 tttttatttt aaaaattttt tcacaaaacg gtttacaagc ataaagctct agagtcgacc
     2581 tgcaggcatg caagcttcga gtccctgctc gtcacgcttt caggcaccgt gccagatatc
     2641 gacgtggagt cgatcactgt gattggcgaa ggggaaggca gcgctaccca aatcgctagc
     2701 ttgctggaga agctgaaaca aaccacgggc attgatctgg cgaaatccct accgggtcaa
     2761 tccgactcgc ccgctgcgaa gtcctaagag atagcgatgt gaccgcgatc gcttgtcaag
     2821 aatcccagtg atcccgaacc ataggaaggc aagctcaatg cttgcctcgt cttgaggact
     2881 atctagatgt ctgtggaacg cacatttatt gccatcaagc ccgatggcgt tcagcggggt
     2941 ttggtcggta cgatcatcgg ccgctttgag caaaaaggct tcaaactggt gggcctaaag
     3001 cagctgaagc ccagtcgcga gctggccgaa cagcactatg ctgtccaccg cgagcgcccc
     3061 ttcttcaatg gcctcgtcga gttcatcacc tctgggccga tcgtggcgat cgtcttggaa
     3121 ggcgaaggcg ttgtggcggc tgctcgcaag ttgatcggcg ctaccaatcc gctgacggca
     3181 gaaccgggca ccatccgtgg tgattttggt gtcaatattg gccgcaacat catccatggc
     3241 tcggatgcaa tcgaaacagc acaacaggaa attgctctct ggtttagccc agcagagcta
     3301 agtgattgga cccccacgat tcaaccctgg ctgtacgaat aaggtctgca ttccttcaga
     3361 gagacattgc catgcccgtg ctgcgatcgc ccttccaagc tgccttgccc cgctgtttcg
     3421 ggctggcagc cctggcgttg gggctggcga ccgcttgcca agaaagcagc gctccgccgg
     3481 ctgccggata tcctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     3541 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     3601 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     3661 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     3721 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     3781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3841 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3901 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3961 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4021 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4081 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4141 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4201 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4261 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4321 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4381 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4441 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4501 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4561 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4621 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4681 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4741 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4801 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4861 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4921 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4981 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     5041 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5101 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5161 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5221 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtcgccgggg
     5281 ctggcagctt agtcctgcgc aatctctact acatctgcca acccagtgaa attttgatct
     5341 ttgctggcag tagtcgccgc agtagtgatg gccgccgagt tggctatcgc ttggtcaagg
     5401 gcggcagcag cctgcgggta cctctgctgg aaaaagcgct ccgcatggat ctgaccaaca
     5461 tgatcattga gttgcgcgtt tccaatgcct tctccaaggg cggcattccc ctgactgttg
     5521 aaggcgttgc caatatcaag attgctgggg aagaaccgac catccacaac gcgatcgagc
     5581 ggctgcttgg caaaaaccgt aaggaaatcg agcaaattgc caaggagacc ctcgaaggca
     5641 acttgcgtgg tgttttagcc agcctcacgc cggagcagat caacgaggac aaaattgcct
     5701 ttgccaaaag tctgctggaa gaggcggagg atgaccttga gcagctgggt ctagtcctcg
     5761 atacgctgca agtccagaac atttccgatg aggtcggtta tctctcggct agtggacgca
     5821 agcagcgggc tgatctgcag cgagatgccc gaattgctga agccgatgcc caggctgcct
     5881 ctgcgatcca aacggccgaa aatgacaaga tcacggccct gcgtcggatc gatcgcgatg
     5941 tagcgatcgc ccaagccgag gccgagcgcc ggattcagga tgcgttgacg cggcgcgaag
     6001 cggtggtggc cgaagctgaa gcggacattg ctaccgaagt cgctcgtagc caagcagaac
     6061 tccctgtgca gcaggagcgg atcaaacagg tgcagcagca acttcaagcc gatgtgatcg
     6121 ccccagctga ggcagcttgt aaacgggcga tcgcggaagc gcggggggcc gccgcccgta
     6181 tcgtcgaaga tggaaaagct caagcggaag ggacccaacg gctggcggag gcttggcaga
     6241 ccgctggtgc taatgcccgc gacatcttcc tgctccagaa gctcgaaatt cgagctcggt
     6301 acccggggat cgatctggcc gcgggtcatg gctgcgcccc gacacccgcc aacacccgct
     6361 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6421 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag
     6481 atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc gaatggtgca
     6541 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg
     6601 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     6661 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     6721 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     6781 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     6841 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     6901 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     6961 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     7021 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     7081 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     7141 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     7201 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     7261 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     7321 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     7381 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     7441 gttatatccc gccgtcaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     7501 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     7561 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     7621 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7681 gagcgcaacg caattaatgt gagttagcgc gaattgatct ggtttgacag cttatcatcg
     7741 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     7801 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     7861 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     7921 ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7981 acagaccatg gaaatgtcta gattagataa aagtaaagtg attaacagcg cattagagct
     8041 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     8101 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     8161 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     8221 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     8281 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     8341 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     8401 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
     8461 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
     8521 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
     8581 attagaaaaa caacttaaat gtgaaagtgg gtctgacatc ctcgagttga tggtttcgaa
     8641 gggcgaagaa ctgtttacgg gagtggtgcc gatcctggtt gagttggatg gcgacgtgaa
     8701 cggacacaag tttagtgtct ccggcgaagg tgagggtgac gctacctacg gtaaactgac
     8761 gctgaaattc atttgtacta ccggtaaact cccggttcca tggcctacgc tggttacgac
     8821 cctcagctgg ggtgtccaat gcttcgcccg ctacccggac cacatgaagc agcacgactt
     8881 tttcaaaagt gccatgcctg aggggtacgt tcaagaacgc accattttct ttaaggatga
     8941 cggcaattac aaaacacgcg cagaggtgaa atttgaaggt gatacgttgg tcaatcgaat
     9001 cgaactcaaa ggcattgact ttaaagagga tggcaatatc ctcggccaca aactggaata
     9061 caattacttc agcgacaacg tctatatcac agctgataag cagaaaaatg ggattaaagc
     9121 caactttaag atccggcata acattgaaga tggcggcgtg caactggcgg atcattatca
     9181 gcagaatact cccatcggcg atgggcccgt cctgttgcca gataaccatt atctgtctac
     9241 ccaaagcaag ttgtcgaagg atcccaacga gaagcgtgat catatggtcc tcttggagtt
     9301 cgtgactgcg gcaggcatta ccctcgggat ggatgaattg tataagtag
//
