LOCUS New_DNA 5700 bp ds-DNA circular 02-MAR-2015 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from E:/ucsd lab/plasmid/ApE maps/pSX257 pCR8-PH-miniSOG.ape 652 to 1445 Backbone from E:/ucsd lab/plasmid/ApE maps/pQA181 Punc-25 GwRfcA.str 4307 to 2624 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 48..417 /label=PH domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green primer_bind complement(886..905) /label=T3 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green CDS complement(3536..3604) /label=LacZ alpha /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue misc_feature 418..437 /label=PH domain(1) /ApEinfo_label=PH domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green misc_recomb 5693..5700 /vntifkey="86" /label=attR1 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_recomb complement(788..794) /label=attB2 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd primer_bind 3675..3692 /label=M13-fwd /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_recomb 1..14 /label=attB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd misc_recomb 1..14 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum primer_bind complement(923..943) /label=M13-rev /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green promoter 3735..5680 /vntifkey="29" /label=Punc-25_yq /ApEinfo_fwdcolor=seashell4 /ApEinfo_revcolor=seashell4 misc_feature 1..7 /label=Gateway recomb1 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 misc_feature 770..770 /label=PH domain(2) /ApEinfo_label=PH domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green primer_bind 3701..3721 /label=T7 /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green misc_feature 768..769 /label=PH domain(3) /ApEinfo_label=PH domain /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=green misc_recomb complement(795..809) /vntifkey="86" /label=attR2 /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink misc_binding complement(949..971) /label=LacO /ApEinfo_fwdcolor=cornflower blue /ApEinfo_revcolor=cornflower blue misc_feature 444..740 /label=miniSOG /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green rep_origin complement(1311..1993) /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 CDS complement(2091..2750) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow misc_feature 741..758 /label=miniSOG(1) /ApEinfo_label=miniSOG /ApEinfo_fwdcolor=#00ff00 /ApEinfo_revcolor=green rep_origin 3089..3529 /label=F1 ori /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 ORIGIN 1 gtacaaaaaa gcaggctccg aattcgccct tcGGATCCAG ATCTATGCAC GGGCTCCAGG 61 ATGACCCGGA CCTTCAGGCC CTTCTGAAGG GCAGCCAGCT TCTGAAGGTG AAGTCCAGCT 121 CGTGGCGTAG GGAACGCTTC TACAAGCTAC AGGAGGACTG CAAGACCATC TGGCAGGAAT 181 CTCGAAAGGT CATGAGGTCC CCGGAGTCGC AGCTGTTCTC CATCGAGGAC ATTCAGGAGG 241 TACGGATGGG ACACCGCACA GAAGGCCTGG AGAAGTTTGC CCGAGACATC CCCGAGGATC 301 GATGCTTCTC CATTGTCTTC AAGGACCAGC GCAACACCCT AGACCTCATT GCCCCATCAC 361 CAGCTGACGC TCAGCACTGG GTGCAGGGCC TGCGCAAGAT CATCCACCAC TCCGGCTCCA 421 TGGACCAGCG GCAGAAGACT AGTGAGAAAA GTTTCGTGAT AACTGATCCA CGGCTGCCAG 481 ACAATCCCAT CATCTTCGCA TCCGATGGCT TCCTGGAGCT GACCGAGTAT TCCAGAGAGG 541 AGATCCTGGG CCGCAATGGC CGCTTTCTGC AGGGACCAGA GACAGACCAG GCCACAGTGC 601 AGAAGATTCG CGATGCCATT AGAGATCAGC GCGAGATTAC CGTGCAGCTG ATAAACTACA 661 CAAAAAGCGG GAAGAAATTC TGGAACCTCC TGCACCTCCA GCCCATGAGG GACCAGAAGG 721 GTGAGCTCCA GTATTTCATC GGAGTGCAGC TGGATGGATG ACTCGAGTAA caagggcgaa 781 ttcgacccag ctttcttgta caaagtggtg atatcgaatt cctgcagccc gggggatcca 841 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg 901 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 1021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 1081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 1141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 1201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 1261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 1321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 1381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 1441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 1501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 1561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 1621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 1681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 1741 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 1801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 1861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 1921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 1981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 2281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 2341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 2401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 2461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 2521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 2581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 2641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 2701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 2761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 2821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 2881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 2941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 3061 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 3121 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 3181 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 3241 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 3301 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 3361 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 3421 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 3481 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt 3541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 3601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 3661 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg 3721 gcgaattggg taccatcatc atgcccgctg ctgttatcag atggatttga aggagttgtt 3781 gttaagaaat gagaaataag aaataattgt ataatttttt tttccaaaaa tttaaatttc 3841 taaactaaaa ttcgaatttt tgcatgcaaa aaacacccac tttttgatct caaatttgca 3901 cttattaagc actaaaaacc aataatttct tccctttaaa atctaaaaaa aatcttccaa 3961 taaccccccc actgataaca acaacaaggc atttttcact cgacgaccgg tggcggagcg 4021 cacgtcttct tattcttctt cttcttcttc ttttcctacc ttttctatcc cactttttcc 4081 ccggaatttt gagatttgac atgatttttc agagaatttc gagttttgag aaaaaaaaaa 4141 tcaaaaaagc gattttttgg tcaaaagccg aaatttaaag ctagtttttt tggaaatatc 4201 gatttttttt ttgagaattt ttcgattttc caaaaaaaaa atcgaaagtt gtttcccgaa 4261 aagccgaaat tttttttgag atatcgaaaa atcggagaat ttttcgatgt ttcccagaaa 4321 tactaaaatt ttgaattttc cggaaaaaaa atcgttaaaa aattagtttc ttttaaagac 4381 cgaaatttaa agtttttttt ttggaaatat cgattttttc cgagatatct aaaattgaaa 4441 gaaattttcg attttctgag aaaaatacga aaaaatcgaa aaaaaaaccc aaatttcggt 4501 tttttcgtaa aaaaagtcgt aaaaatgtaa tttttttcct gaaaaatcgg aattttttac 4561 aaaatatcgg aaaatactca aaaaaaagct gaaaatttcg attttccgga gaaaaaatct 4621 ttttaaaaaa atattttttt tttcagaaaa taagaaaagc cgaaatttat aactatttct 4681 ccggaaattc gaaattttta acgaaatatc ggaataattt ttagattttt caagtttgac 4741 tttgcgagaa aaaaatcgga aaaacctcga ttcgacgccg aaaaatgctc cttttcgaaa 4801 agatttttga aatttcagaa aatcgacatg caagcgcgct ccacggcgaa atgacaacga 4861 tgatccaccg ccctcaaaaa gttgggtctc gttaggtatt tggcggtaaa actggtaaaa 4921 ctccagtttt gcctccaacg agacccaatt ttttggggcg gtggtggagc gcgcttgcac 4981 aagctgaaag catttttctg cgactcgata atattttgaa aacctgtgtc aattctcgaa 5041 attctttttt taaaaaataa tcccgagctt ctctcagtcc tcctctatga ggatgttcct 5101 tttttttggt ttttcaattt tttttaaaat tccaaatttc tgttgtgcaa ttcacttccc 5161 cccaagaaat cccaaaaatc cccagttttc cccaaaaatg ttccgttttc atgtgatttt 5221 tcccccattt ttaaaacatt tttttgactt ttttttaaaa tgattattat tattgttttc 5281 tatttcatgg ccggtaaatt attttttttc tttctttttt tttgctcttt tttttcaaga 5341 attttcgaat tgtttgaagg gctgctcatc taatcttttg tcattttgtt ctgatgccat 5401 catttctgag aggacctttg aagactcgtc acgaaacggg aggggggctc aagtgagcat 5461 tattattatt attattgtcg caaaaagttt accccgggct ccccctggct cccctctttg 5521 agcaagggtt taagggctca ttttgatgac gaattgctca ttgggattat agtcacgccc 5581 ctcttttgga gcaactacac aactgagcca cagtaatcct tgggggcggg gtcagtagga 5641 ccccctccgg aatagggaaa agctcagttc accgccaaaa aagcttgata tcacaagttt //