LOCUS       Exported                5669 bp ds-DNA     circular SYN 05-APR-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    87763-attachment_Ls-InptbMASk (1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 5, 2017 from SnapGene 3.3.3
            http://www.snapgene.com
COMMENT     /Users/Tammy/Documents/Jin Lab/DNA/gateway clones/p-acr-2 gateway 
            pqa235.ape from 1 to 7363
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(135..441)
                     /direction=LEFT
                     /label=F1 ori
                     /note="F1 ori"
                     /note="ApEinfo_fwdcolor=gray50
                     ApEinfo_revcolor=gray50"
                     /note="color: #808080"
     CDS             complement(460..528)
                     /label=LacZ alpha
                     /note="LacZ alpha"
                     /note="ApEinfo_fwdcolor=#6495ed
                     ApEinfo_revcolor=#6495ed"
                     /note="color: #6495ed; direction: LEFT"
     misc_feature    497..502
                     /label=pvui
                     /note="pvui"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     primer_bind     599..616
                     /label=M13-fwd
                     /note="M13-fwd"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff; direction: RIGHT"
     primer_bind     625..645
                     /label=T7
                     /note="T7"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    653..1748
                     /label=pacr-2
                     /note="pacr-2"
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_revcolor=green"
                     /note="This feature has 3 segments:
                        1:  653 ..  658 / #00ff00
                        2:  659 .. 1736 / #00ff00
                        3: 1737 .. 1748 / #00ff00"
     misc_feature    653..658
                     /label=kpn1
                     /note="kpn1"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    653..658
                     /label=KPNI
                     /note="KPNI"
                     /note="ApEinfo_fwdcolor=#a181ff
                     ApEinfo_revcolor=green"
                     /note="color: #a181ff"
     misc_feature    659..1736
                     /label=pnmr-1
                     /note="pnmr-1"
                     /note="ApEinfo_fwdcolor=#7493ff
                     ApEinfo_revcolor=green"
                     /note="color: #7493ff"
     misc_feature    659..682
                     /label=5' primer
                     /note="5' primer"
                     /note="ApEinfo_fwdcolor=#7b76ff
                     ApEinfo_revcolor=green"
                     /note="color: #7b76ff"
     misc_feature    1709..1736
                     /label=3' primer
                     /note="3' primer"
                     /note="ApEinfo_fwdcolor=#a798ff
                     ApEinfo_revcolor=green"
                     /note="color: #a798ff"
     misc_feature    1737..1875
                     /label=synthetic intron
                     /note="synthetic intron"
                     /note="ApEinfo_fwdcolor=#ff7fad
                     ApEinfo_revcolor=green"
                     /note="color: #ff7fad"
     misc_feature    1737..1742
                     /label=HINDIII
                     /note="HINDIII"
                     /note="ApEinfo_fwdcolor=#c48eff
                     ApEinfo_revcolor=green"
                     /note="color: #c48eff"
     misc_feature    1743..1748
                     /label=sph1
                     /note="sph1"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_feature    1743..1748
                     /label=hindIII
                     /note="hindIII"
                     /note="ApEinfo_fwdcolor=#cd94ff
                     ApEinfo_revcolor=green"
                     /note="color: #cd94ff"
     misc_feature    1883..1899
                     /label=attrI
                     /note="attrI"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_recomb     1883..1899
                     /label=attB1(1)
                     /note="attB1(1)"
                     /note="ApEinfo_label=attB1
                     ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_revcolor=#dda0dd"
                     /note="color: #dda0dd"
     misc_feature    1890..1896
                     /label=Gateway recomb1
                     /note="Gateway recomb1"
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_revcolor=#ff0000"
                     /note="color: #ff0000"
     misc_recomb     1900..1903
                     /label=attB1
                     /note="attB1"
                     /note="ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_revcolor=#dda0dd"
                     /note="color: #dda0dd"
     misc_recomb     1900..1903
                     /label=AttL1
                     /note="AttL1"
                     /note="ApEinfo_fwdcolor=plum
                     ApEinfo_revcolor=plum"
                     /note="color: #dda0dd"
     misc_feature    1937..2306
                     /label=PH domain
                     /note="PH domain"
                     /note="ApEinfo_fwdcolor=#00ff80
                     ApEinfo_revcolor=green"
                     /note="color: #00ff80"
     misc_feature    2307..2326
                     /label=PH domain(1)
                     /note="PH domain(1)"
                     /note="ApEinfo_label=PH domain
                     ApEinfo_fwdcolor=#00ff80
                     ApEinfo_revcolor=green"
                     /note="color: #00ff80"
     misc_feature    2333..2629
                     /label=miniSOG
                     /note="miniSOG"
                     /note="ApEinfo_fwdcolor=#00ff00
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00"
     misc_feature    2630..2647
                     /label=miniSOG(1)
                     /note="miniSOG(1)"
                     /note="ApEinfo_label=miniSOG
                     ApEinfo_fwdcolor=#00ff00
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00"
     misc_feature    2657..2658
                     /label=PH domain(3)
                     /note="PH domain(3)"
                     /note="ApEinfo_label=PH domain
                     ApEinfo_fwdcolor=#00ff80
                     ApEinfo_revcolor=green"
                     /note="color: #00ff80"
     misc_feature    2659
                     /label=PH domain(2)
                     /note="PH domain(2)"
                     /note="ApEinfo_label=PH domain
                     ApEinfo_fwdcolor=#00ff80
                     ApEinfo_revcolor=green"
                     /note="color: #00ff80"
     misc_recomb     complement(2677..2680)
                     /label=attB2
                     /note="attB2"
                     /note="ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_revcolor=#dda0dd"
                     /note="color: #dda0dd; direction: LEFT"
     misc_feature    2681..2702
                     /label=attR2
                     /note="attR2"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ffff"
     misc_recomb     complement(2681..2701)
                     /label=attB2(1)
                     /note="attB2(1)"
                     /note="ApEinfo_label=attB2
                     ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_revcolor=#dda0dd"
                     /note="color: #dda0dd; direction: LEFT"
     misc_feature    complement(2688..2694)
                     /label=Gateway Recomb2
                     /note="Gateway Recomb2"
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_revcolor=#ff0000"
                     /note="color: #ff0000; direction: LEFT"
     primer_bind     complement(3479..3498)
                     /label=T3
                     /note="T3"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     complement(3516..3536)
                     /label=M13-rev
                     /note="M13-rev"
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_revcolor=green"
                     /note="color: #00ff00; direction: LEFT"
     misc_binding    complement(3542..3564)
                     /label=LacO
                     /note="LacO"
                     /note="ApEinfo_fwdcolor=#6495ed
                     ApEinfo_revcolor=#6495ed"
                     /note="color: #6495ed; direction: LEFT"
     rep_origin      complement(3904..4586)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="ColE1 origin"
                     /note="ApEinfo_fwdcolor=gray50
                     ApEinfo_revcolor=gray50"
                     /note="color: #808080"
     CDS             complement(4684..5343)
                     /label=AmpR
                     /note="AmpR"
                     /note="ApEinfo_fwdcolor=yellow
                     ApEinfo_revcolor=yellow"
                     /note="color: #ffff00; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt
      661 ctgaaagtat aagatttgac tgaaaattat aaaattatga acttgagaaa gtctagaaat
      721 caagaaaaaa tatctagaaa tgaacattcc atgggttgtg aagatgcttt tatctcactt
      781 tagaatgtct gactccgccc ctctgtgtac gaattgaagt gctcttttgt gatgatcata
      841 gctcctcctt gcggcgacgc ccgcacacag cacgagatat tacacggaat acttatgtga
      901 ttcataggaa acaaggaaat cgaaaaaata ataggatttt gaggaggtat ttttagatga
      961 atgcttatga tgttatgatg aacccaagaa attctgaata gacatatgtc agaacggggt
     1021 aggagctcca gtgtcgcttt tgaaataaaa aatcaagtgg tatacttctc gtatataccc
     1081 ttctgaacta ataaaataat ttatcttttg aaaaagtttg aaaaagaaaa atgcccaaaa
     1141 cttgaatttt aatgttttgt attttgagca tatcgttcaa acgtcgtgat ctgaaagtag
     1201 aaagatctaa atattcgtga cacagtttgg tttgaaggtc ttagtggtaa ttttttatta
     1261 cagaagcaac aaaaagttga catattttca gaatttccaa aaaaacgttt aaaatttcaa
     1321 tttcttcgat agagttctaa aaattttttg gaacggattt tgtaatatcc gctgataacc
     1381 gttcaagaac aatttgaatt ttgaaataat ttaatcaact agtatccagt gctctttctc
     1441 actgctccac ccatttttca acccatttcc tttactcaat ttcgcccgtt ttccttttgc
     1501 tcaaatgtgc ccaccaaatc atctttcttc gtcttctttt ctcttattcg gaaggagaag
     1561 aggtgaaagt agaatacgtt tttttgttga aaagagaaga gcactgccac ttttctctta
     1621 tttccatcct tctactacct cgttttactt tccatttaaa aacccgttta aagttccaaa
     1681 atttcaatta tcttgcttct gtttggaaca cgacaaactt tagttttgtt acagataagc
     1741 ttgcatgcct gcaggtcgac tctagaggat ccccgggatt ggccaaagga cccaaaggta
     1801 tgtttcgaat gatactaaca taacatagaa cattttcagg aggacccttg gagggtaccg
     1861 gtagaaaaaa agcttgatat cacaagtttg tacaaaaaag caggctccga attcgccctt
     1921 cggatccaga tctatgcacg ggctccagga tgacccggac cttcaggccc ttctgaaggg
     1981 cagccagctt ctgaaggtga agtccagctc gtggcgtagg gaacgcttct acaagctaca
     2041 ggaggactgc aagaccatct ggcaggaatc tcgaaaggtc atgaggtccc cggagtcgca
     2101 gctgttctcc atcgaggaca ttcaggaggt acggatggga caccgcacag aaggcctgga
     2161 gaagtttgcc cgagacatcc ccgaggatcg atgcttctcc attgtcttca aggaccagcg
     2221 caacacccta gacctcattg ccccatcacc agctgacgct cagcactggg tgcagggcct
     2281 gcgcaagatc atccaccact ccggctccat ggaccagcgg cagaagacta gtgagaaaag
     2341 tttcgtgata actgatccac ggctgccaga caatcccatc atcttcgcat ccgatggctt
     2401 cctggagctg accgagtatt ccagagagga gatcctgggc cgcaatggcc gctttctgca
     2461 gggaccagag acagaccagg ccacagtgca gaagattcgc gatgccatta gagatcagcg
     2521 cgagattacc gtgcagctga taaactacac aaaaagcggg aagaaattct ggaacctcct
     2581 gcacctccag cccatgaggg accagaaggg tgagctccag tatttcatcg gagtgcagct
     2641 ggatggatga ctcgagtaac aagggcgaat tcgacccagc cagctttctt gtacaaagtg
     2701 gtgatatcga attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg
     2761 ccgctgtcat cagatcgcca tctcgcgccc gtgcctctga cttctaagtc caattactct
     2821 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     2881 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     2941 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     3001 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     3061 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     3121 ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc
     3181 tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc
     3241 aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat
     3301 tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca
     3361 cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag
     3421 ttgataattt gaaagtggag tagtgtctat ggggtttttg ccgagctcca gcttttgttc
     3481 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3541 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3601 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3721 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3901 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3961 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4021 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4081 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4141 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4201 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4261 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4321 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4381 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4441 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4501 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4561 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4621 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4681 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4741 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4801 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4861 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4921 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4981 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5041 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5101 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5161 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5221 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5281 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5341 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5401 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5461 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5521 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5581 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5641 tccgcgcaca tttccccgaa aagtgccac
//
